Invitrogen

GFP Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

16 Published Figures

19 References

View all (114) GFP antibodies

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GFP Antibody (PA1-980A) in WB

Western Blot was performed using Anti-GFP Polyclonal Antibody (Product # PA1-980A) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), H3-GFP, 40 µg (Lane 3), H3-GFP, 20 µg (Lane 4), H3-GFP, 10 µg (Lane 5), p65-GFP, 40 µg (Lane 6), His-p65-YFP, 40 µg (Lane 7), H3-mCherry, 40 µg (Lane 8) and 25 ng Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then trans... View More

GFP Antibody in Immunocytochemistry (ICC/IF)

GFP Antibody in Immunohistochemistry (IHC)

GFP Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

GFP Antibody — Antibody specificity was demonstrated by detection of different targets fused to GFP tag in transiently transfected lysates tested. Relative detection of GFP tag was observed across different proteins fused with GFP in H3-GFP (Lane 3-5) and p65-GFP (Lane 6). GFP-variant, YFP is also being detected in His-p65-YFP lysate (Lane 7), using Anti-GFP Polyclonal Antibody (Product # PA1-980A) in Western Blot. {RE}

Product Details

PA1-980A

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25 - 0.5 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: H(26) K F S V S G E G E G D A T(39) C

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325960

Product Specific Information

PA1-980A detects Green Fluorescent Protein (GFP).

PA1-980A has been successfully used in Western blot and immunofluoresence procedures. By Western blot, this antibody detects recombinant GFP.

PA1-980A immunizing peptide corresponds to the N-terminal amino acid residues 26-39 of Aequorea victoria wild-type GFP. This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. PA1-980A immunizing peptide (Cat. # PEP-033) is available for use in neutralization and control experiments.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.

PLoS genetics

Lesko MA,Chandrashekarappa DG,Jordahl EM,Oppenheimer KG,Bowman RW,Shang C,Durrant JD,Schmidt MC,O'Donnell AF

Published figure using GFP polyclonal antibody (Product # PA1-980A) in Western Blot

Mon May 01 00:00:00 EDT 2023

Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.

PLoS genetics

Lesko MA,Chandrashekarappa DG,Jordahl EM,Oppenheimer KG,Bowman RW,Shang C,Durrant JD,Schmidt MC,O'Donnell AF

Published figure using GFP polyclonal antibody (Product # PA1-980A) in Western Blot

Mon May 01 00:00:00 EDT 2023

Citrus sudden death-associated virus as a new expression vector for rapid in planta production of heterologous proteins, chimeric virions, and virus-like particles.

Biotechnology reports (Amsterdam, Netherlands)

Matsumura EE,Guo F,Boogers D,van Oevelen D,Vu ST,Falk BW

PA1-980A was used in Western Blot to show though that the incorporation of GFP-CPp21 fusion protein into virions occurs solely due to its interaction with free/non-fused CPp21, independent of other viral proteins.

Thu Sep 01 00:00:00 EDT 2022

Citrus sudden death-associated virus as a new expression vector for rapid in planta production of heterologous proteins, chimeric virions, and virus-like particles.

Biotechnology reports (Amsterdam, Netherlands)

Matsumura EE,Guo F,Boogers D,van Oevelen D,Vu ST,Falk BW

Thu Sep 01 00:00:00 EDT 2022

The plastoglobule-localized protein AtABC1K6 is a Mn²⁺-dependent kinase necessary for timely transition to reproductive growth.

The Journal of biological chemistry

Espinoza-Corral R,Lundquist PK

PA1-980A was used in Western Blot to demonstrate in vitro autophosphorylation activity, which we replicate with a human ABC1 ortholog.

Fri Apr 01 00:00:00 EDT 2022

The plastoglobule-localized protein AtABC1K6 is a Mn²⁺-dependent kinase necessary for timely transition to reproductive growth.

The Journal of biological chemistry

Espinoza-Corral R,Lundquist PK

PA1-980A was used in Western Blot to demonstrate in vitro autophosphorylation activity, which we replicate with a human ABC1 ortholog.

Fri Apr 01 00:00:00 EDT 2022

Integrative Role of 14-3-3ε in Sleep Regulation.

International journal of molecular sciences

Wei Y,Du J,Zhao Z

PA1-980A was used in Immunohistochemistry to find that 14-3-3ε, expression of which is activated by the circadian transcription factor Clock, is necessary for sleep regulation in Drosophila and elucidate it's mechanism of action.

Thu Sep 09 00:00:00 EDT 2021

Integrative Role of 14-3-3ε in Sleep Regulation.

International journal of molecular sciences

Wei Y,Du J,Zhao Z

Thu Sep 09 00:00:00 EDT 2021

Development of a Honey Bee RNA Virus Vector Based on the Genome of a Deformed Wing Virus.

Viruses

Ryabov EV,Christmon K,Heerman MC,Posada-Florez F,Harrison RL,Chen Y,Evans JD

PA1-980A was used in Western Blotting to develop a honey bee RNA-virus vector based on the genome of a picorna-like Deformed wing virus (DWV), the main viral pathogen of the honey bee (Apis mellifera).

Sat Mar 28 00:00:00 EDT 2020

Development of a Honey Bee RNA Virus Vector Based on the Genome of a Deformed Wing Virus.

Viruses

Ryabov EV,Christmon K,Heerman MC,Posada-Florez F,Harrison RL,Chen Y,Evans JD

Sat Mar 28 00:00:00 EDT 2020

Pathogenic E. coli Extracts Nutrients from Infected Host Cells Utilizing Injectisome Components.

Cell

Pal RR,Baidya AK,Mamou G,Bhattacharya S,Socol Y,Kobi S,Katsowich N,Ben-Yehuda S,Rosenshine I

PA1-980A was used in Western Blotting to show that host-attached bacteria obtain nutrients from infected host cell in a process we termed host nutrient extraction.

Thu Apr 18 00:00:00 EDT 2019

Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells.

Nature communications

Valsecchi CIK,Basilicata MF,Semplicio G,Georgiev P,Gutierrez NM,Akhtar A

Published figure using GFP polyclonal antibody (Product # PA1-980A) in Immunofluorescence

Fri Sep 07 00:00:00 EDT 2018

Cardiac Electrophysiological Effects of Light-Activated Chloride Channels.

Frontiers in physiology

Kopton RA,Baillie JS,Rafferty SA,Moss R,Zgierski-Johnston CM,Prykhozhij SV,Stoyek MR,Smith FM,Kohl P,Quinn TA,Schneider-Warme F

PA1-980A was used in Immunohistochemistry to study the cardiac electrophysiological effects of light-activated chloride channels.

Thu Oct 01 00:00:00 EDT 2020

Variations in the multimerization region of the Helicobacter pylori cytotoxin CagA affect virulence.

Oncology letters

Ahire D,Alston T,Raffaniello R

Published figure using GFP Tag polyclonal antibody (Product # PA1-980A) in Western Blot

Wed Mar 01 00:00:00 EST 2017

Heterodimers of tyrosylprotein sulfotransferases suggest existence of a higher organization level of transferases in the membrane of the trans-Golgi apparatus.

Journal of molecular biology

Hartmann-Fatu C,Trusch F,Moll CN,Michin I,Hassinen A,Kellokumpu S,Bayer P

PA1-980A was used in immunocytochemistry to investigate the localization and binding partners of the tyrosyl protein sulfotransferases, TPST1 and TPST2

Fri Mar 27 00:00:00 EDT 2015

Liver X receptor ligand cytotoxicity in colon cancer cells and not in normal colon epithelial cells depends on LXRβ subcellular localization.

Oncotarget

Courtaut F,Derangère V,Chevriaux A,Ladoire S,Cotte AK,Arnould L,Boidot R,Rialland M,Ghiringhelli F,Rébé C

PA1-980A was used in western blot to investigate LXRbeta signaling in colon cancer cells

Tue Sep 29 00:00:00 EDT 2015

Liver X receptor ligand cytotoxicity in colon cancer cells and not in normal colon epithelial cells depends on LXRβ subcellular localization.

Oncotarget

Courtaut F,Derangère V,Chevriaux A,Ladoire S,Cotte AK,Arnould L,Boidot R,Rialland M,Ghiringhelli F,Rébé C

PA1-980A was used in western blot to investigate LXRbeta signaling in colon cancer cells

Tue Sep 29 00:00:00 EDT 2015

Probing protein interactions in living mammalian cells on a microtubule bench.

Scientific reports

Boca M,Kretov DA,Desforges B,Mephon-Gaspard A,Curmi PA,Pastré D

PA1-980A was used in western blot to develop a method to use microtubules as platforms to detect protein interactions in mammalian cells

Fri Nov 27 00:00:00 EST 2015

Development of a tightly regulated and highly responsive copper-inducible gene expression system and its application to control of flowering time.

Plant cell reports

Saijo T,Nagasawa A

PA1-980A was used in western blot to study the ability to modulate flowering time using a novel copper-inducible expression system

Wed Jan 01 00:00:00 EST 2014

Development of a continuous assay for the measurement of tissue factor procoagulant activity on intact cells.

Laboratory investigation; a journal of technical methods and pathology

Caldwell JA,Dickhout JG,Al-Hashimi AA,Austin RC

PA1-980A was used in blocking or activating experiment to evaluate a novel assay for the continuous measurement of tissue factor procoagulant activity in intact cells

Tue Jun 01 00:00:00 EDT 2010

A novel method for the production of transgenic cloned pigs: electroporation-mediated gene transfer to non-cultured cells and subsequent selection with puromycin.

Biology of reproduction

Watanabe S,Iwamoto M,Suzuki S,Fuchimoto D,Honma D,Nagai T,Hashimoto M,Yazaki S,Sato M,Onishi A

PA1-980A was used in western blot to evaluate a novel way to produe transgenic cloned pigs

Tue Feb 01 00:00:00 EST 2005

An amino-terminal motif functions as a second nuclear export sequence in BRCA1.

The Journal of biological chemistry

Thompson ME,Robinson-Benion CL,Holt JT

PA1-980A was used in western blot to identify nuclear export sequences in BRAC1 gene .

Fri Jun 10 00:00:00 EDT 2005

Unfolding-resistant translocase targeting: a novel mechanism for outer mitochondrial membrane localization exemplified by the Bbeta2 regulatory subunit of protein phosphatase 2A.

The Journal of biological chemistry

Dagda RK,Barwacz CA,Cribbs JT,Strack S

PA1-980A was used in western blot to study the localization of heterotrimeric serine/threonine protein phosphatase 2A beta2 subunit.

Fri Jul 22 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GFP Polyclonal Antibody

GFP Antibody (PA1-980A) in WB

Product Details

PA1-980A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Neutralization (Neu)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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