Invitrogen

GFP Monoclonal Antibody (GF28R), eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4 Published Figures

11 References

View all (114) GFP antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GFP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

GFP Antibody (14-6674-82) in ICC/IF

Immunofluorescent analysis of GFP Tag was performed using H3-GFP construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GFP Monoclonal Antibody (GF28R), eBioscience™ (Product # 14-6674-82) at 1:100 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat... View More

Product Image Gallery

GFP Antibody — Antibody specificity was demonstrated by detection of different targets fused to GFP tag in transiently transfected lysates tested. Relative detection of GFP tag was observed across different proteins fused with GFP in H3-GFP (Lane 3-5) and p65-GFP (Lane 6). GFP-variant, YFP is also being detected in His-p65-YFP lysate (Lane 7), using Anti-GFP Monoclonal Antibody (GF28R), eBioscience™ (Product # 14-6674-82) in Western Blot. {RE}

Product Details

14-6674-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GF28R

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572900

Product Specific Information

Description: This GF28R monoclonal antibody reacts with green-fluorescent protein (GFP), which was originally isolated from the cnidarian Aequorea victoria. This 27-kDa protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509) without the requirement of exogenous substrates and cofactors. These unique qualities allow GFP to be used to monitor gene expression and protein localization in vivo. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maxima when compared to wildtype GFP, making them useful for flow cytometry, fluorescence microscopy, and double-labeling applications. This antibody is capable of staining formaldehyde fixed cells.

Applications Reported: This GF28R antibody has been reported for use in immunoprecipitation, and western blotting.

Applications Tested: This GF28R antibody has been tested by immunoblotting of reduced cell lysate expressing GFP at less than or equal to 1 µg/ mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.

Human molecular genetics

Lin BC,Phung TH,Higgins NR,Greenslade JE,Prado MA,Finley D,Karbowski M,Polster BM,Monteiro MJ

14-6674-82 was used in Immunocytochemistry-immunoflourescence to indicate a potential key pathogenic disturbance in mitochondrial health caused by UBQLN2 mutations.

Thu Jun 17 00:00:00 EDT 2021

Gene knockdown in malaria parasites via non-canonical RNAi.

Nucleic acids research

Hentzschel F,Mitesser V,Fraschka SA,Krzikalla D,Carrillo EH,Berkhout B,Bártfai R,Mueller AK,Grimm D

Published figure using GFP monoclonal antibody (Product # 14-6674-82) in Western Blot

Fri Jan 10 00:00:00 EST 2020

KSHV LANA acetylation-selective acidic domain reader sequence mediates virus persistence.

Proceedings of the National Academy of Sciences of the United States of America

Juillard F,de Miranda MP,Li S,Franco A,Seixas AF,Liu B,Álvarez ÁL,Tan M,Szymula A,Kaye KM,Simas JP

14-6674 was used in Western Blotting to implicate an acetylation-dependent mechanism mediating KSHV persistence and expand the role of acidic domain readers.

Tue Sep 08 00:00:00 EDT 2020

A protein quality control pathway regulated by linear ubiquitination.

The EMBO journal

van Well EM,Bader V,Patra M,Sánchez-Vicente A,Meschede J,Furthmann N,Schnack C,Blusch A,Longworth J,Petrasch-Parwez E,Mori K,Arzberger T,Trümbach D,Angersbach L,Showkat C,Sehr DA,Berlemann LA,Goldmann P,Clement AM,Behl C,Woerner AC,Saft C,Wurst W,Haass C,Ellrichmann G,Gold R,Dittmar G,Hipp MS,Hartl FU,Tatzelt J,Winklhofer KF

14-6674 was used in Immunocytochemistry-immunoflourescence to show the linear ubiquitin chain assembly complex modifies disease-associated protein aggregates.

Thu May 02 00:00:00 EDT 2019

Measles Virus Ribonucleoprotein Complexes Rapidly Spread across Well-Differentiated Primary Human Airway Epithelial Cells along F-Actin Rings.

mBio

Singh BK,Pfaller CK,Cattaneo R,Sinn PL

14-6674 was used in Western Blotting to use unpassaged, well-differentiated primary cultures of airway epithelial cells from human donor lungs to examine measles virus (MeV) infection and spread.

Tue Nov 26 00:00:00 EST 2019

The homeobox protein VentX reverts immune suppression in the tumor microenvironment.

Nature communications

Le Y,Gao H,Bleday R,Zhu Z

14-6674 was used in Western Blotting to show that VentX promotes M1 differentiation of tumor-associated macrophages (TAMs), and that VentX-regulated TAMs, in turn, revert immune suppression at the tumor microenvironment.

Tue Jun 05 00:00:00 EDT 2018

Systems-guided forward genetic screen reveals a critical role of the replication stress response protein ETAA1 in T cell clonal expansion.

Proceedings of the National Academy of Sciences of the United States of America

Miosge LA,Sontani Y,Chuah A,Horikawa K,Russell TA,Mei Y,Wagle MV,Howard DR,Enders A,Tscharke DC,Goodnow CC,Parish IA

14-6674 was used in Western Blotting to identify a crucial role for ETAA1 in this process by surveying random germ line mutations in mice using exome sequencing and bioinformatic annotation to prioritise mutations in genes of unknown function with potential effects on the immune system.

Tue Jun 27 00:00:00 EDT 2017

A novel ALS-associated variant in UBQLN4 regulates motor axon morphogenesis.

eLife

Edens BM,Yan J,Miller N,Deng HX,Siddique T,Ma YC

14-6674 was used in Western Blotting to provide a link between the regulation of axonal morphogenesis and a new amyotrophic lateral sclerosis-associated gene variant mediated by protein degradation pathways.

Tue May 02 00:00:00 EDT 2017

WDR23 regulates NRF2 independently of KEAP1.

PLoS genetics

Lo JY,Spatola BN,Curran SP

14-6674 was used in Western Blotting to identify WDR23 as an alternative regulator of NRF2 proteostasis and uncover a cellular pathway that regulates NRF2 activity and capacity for cytoprotection independently of KEAP1.

Sat Apr 01 00:00:00 EDT 2017

Human mast cells capture, store, and release bioactive, exogenous IL-17A.

Journal of leukocyte biology

Noordenbos T,Blijdorp I,Chen S,Stap J,Mul E,Cañete JD,Lubberts E,Yeremenko N,Baeten D

Published figure using GFP monoclonal antibody (Product # 14-6674-82) in Immunocytochemistry

Thu Sep 01 00:00:00 EDT 2016

Measles Virus Defective Interfering RNAs Are Generated Frequently and Early in the Absence of C Protein and Can Be Destabilized by Adenosine Deaminase Acting on RNA-1-Like Hypermutations.

Journal of virology

Pfaller CK,Mastorakos GM,Matchett WE,Ma X,Samuel CE,Cattaneo R

14-6674 was used in Western Blotting to show by coinfection that the C-defective phenotype is dominant.

Sat Aug 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP

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