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GITRL Antibody (500-P244-1MG) in ELISA
Sandwich ELISA: To detect Human AITRL by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of GITRL Polyclonal Antibody (Product # 500-P244-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech GITRL Polyclonal Antibody, Biotin (Product # 500-P244BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human AITRL.
Product Details
500-P244-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human AITRL
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930020
Product Specific Information
AA Sequence of recombinant protein: METAKEPCMA KFGPLPSKWQ MASSEPPCVN KVSDWKLEIL QNGLYLIYGQ VAPNANYNDV APFEVRLYKN KDMIQTLTNK SKIQNVGGTY ELHVGDTIDL IFNSEHQVLK NNTYWGIILL ANPQFIS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human AITRL. Anti-Human AITRL-specific antibody was purified by affinity chromatography employing an immobilized Human AITRL matrix.
Sandwich ELISA: To detect Human AITRL by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human AITRL (500-P244Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human AITRL.
Western Blot: To detect Human AITRL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human AITRL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-kappa-B and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells and can activate NF-kappa-B, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-kappa-B activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activation-inducible TNF-related ligand; AITR ligand; AITRL; GITR ligand; GITRLigand; Glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand; hGITRL; RP1-15D23.1; TNF superfamily member 18; tumor necrosis factor (ligand) superfamily member 18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand 2A; Tumor necrosis factor ligand superfamily member 18
Gene Aliases: AITRL; GITRL; hGITRL; TL6; TNFSF18; TNLG2A; UNQ149/PRO175
UniProt ID: (Human) Q9UNG2
Entrez Gene ID: (Human) 8995
adaptive immune response
T cell proliferation involved in immune response
positive regulation of leukocyte migration
signal transduction
cell-cell signaling
positive regulation of macrophage chemotaxis
tumor necrosis factor-mediated signaling pathway
regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
negative regulation of apoptotic process
positive regulation of cell adhesion
positive regulation of inflammatory response
positive regulation of NF-kappaB transcription factor activity
positive regulation of monocyte chemotaxis
positive regulation of protein homodimerization activity
negative regulation of T-helper 17 cell lineage commitment
regulation of dendritic cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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