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GLI1 Antibody (MA5-38530) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of GLI1 in HeLa cells using GLI1 Monoclonal Antibody (Product # MA5-38530) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Product Details
MA5-38530
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1B9F8
Immunogen
Purified recombinant fragment of human GLI1 (aa: 284-449) expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898442
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLI-Kruppel family member GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; glioma-associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; Zinc finger protein GLI1
Gene Aliases: GLI; GLI1
UniProt ID: (Human) P08151
Entrez Gene ID: (Human) 2735
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
chromatin binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
microtubule binding
transcription regulatory region DNA binding
metal ion binding
DNA-binding transcription factor
osteoblast differentiation
transcription, DNA-templated
smoothened signaling pathway
spermatogenesis
ventral midline development
positive regulation of cell proliferation
regulation of smoothened signaling pathway
response to wounding
epidermal cell differentiation
dorsal/ventral pattern formation
proximal/distal pattern formation
cerebellar cortex morphogenesis
smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation
pituitary gland development
lung development
regulation of osteoblast differentiation
positive regulation of DNA replication
positive regulation of smoothened signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
digestive tract morphogenesis
notochord regression
canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
liver regeneration
regulation of hepatocyte proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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