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GLI2 Antibody (600-401-845) in IHC (P)
Rocklands Affinity Purified anti-Gli2 antibody shows strong cytoplasmic and membranous staining of tumor cells in human breast tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
Product Details
600-401-845
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chimpanzee,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human Gli-2 (isoform a).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human Gli-2 protein. A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.
Target Information
Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLI family zinc finger protein 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; oncogene GLI2; Tax helper protein; tax helper protein 1; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; Zinc finger protein GLI2
Gene Aliases: CJS; GLI2; HPE9; PHS2; THP; THP1; THP2
UniProt ID: (Human) Q15591
Entrez Gene ID: (Human) 2736
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
in utero embryonic development
kidney development
chondrocyte differentiation
osteoblast development
transcription from RNA polymerase II promoter
smoothened signaling pathway
pattern specification process
axon guidance
ventral midline development
hindgut morphogenesis
heart development
cell proliferation
regulation of smoothened signaling pathway
response to mechanical stimulus
anterior/posterior pattern specification
proximal/distal pattern formation
floor plate formation
spinal cord dorsal/ventral patterning
ventral spinal cord development
cerebellar cortex morphogenesis
smoothened signaling pathway involved in ventral spinal cord interneuron specification
smoothened signaling pathway involved in spinal cord motor neuron cell fate specification
smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation
spinal cord ventral commissure morphogenesis
pituitary gland development
lung development
mammary gland development
hindbrain development
negative regulation of chondrocyte differentiation
positive regulation of T cell differentiation in thymus
tube development
odontogenesis of dentin-containing tooth
embryonic digit morphogenesis
negative regulation of apoptotic process
positive regulation of neuron differentiation
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic digestive tract development
developmental growth
neuron development
branching morphogenesis of an epithelial tube
notochord regression
prostatic bud formation
mammary gland duct morphogenesis
smoothened signaling pathway involved in dorsal/ventral neural tube patterning
cellular response to organic cyclic compound
cochlea morphogenesis
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