Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
GLI2 Antibody (PA5-93021) in WB
Western blot analysis of GLI2 in extracts of various cell lines. Samples were incubated with GLI2 Polyclonal antibody (Product # PA5-93021) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-93021
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of mus musculus GLI2 (NP_0010745941)
Conjugate
Form
Liquid
Concentration
0.23 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806593
Product Specific Information
Positive Samples: Mouse lung, Mouse brain, Mouse heart, Mouse ovary, Mouse pancreas, Rat brain; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: METSAPAPAL EKKEAKSGLL EDSSFPDPGK KACPLAVAAA VAAHGVPQQL LPAFHAPLPI DMRHQEGRYH YDPHSVHSVH GPPTLSGSPV ISDISLIRLS PHPAGPGESP FSAHHPYVNP HMEHYLRSVH SSPTLSMISA ARGLSPADVA HEHLKERGLF SLAAPGTNPS DYYHQ
Target Information
Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: GLI-Kruppel family member GLI2; Tax helper protein; Zinc finger protein GLI2
Gene Aliases: AW546128; Gli2; Thp
UniProt ID: (Mouse) Q0VGT2
Entrez Gene ID: (Rat) 304729, (Mouse) 14633
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
molecular_function
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
zinc ion binding
sequence-specific DNA binding
transcription regulatory region DNA binding
nucleic acid binding
protein binding
metal ion binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
osteoblast differentiation
in utero embryonic development
kidney development
morphogenesis of an epithelium
chondrocyte differentiation
osteoblast development
transcription from RNA polymerase II promoter
smoothened signaling pathway
pattern specification process
axon guidance
ventral midline development
hindgut morphogenesis
heart development
cell proliferation
positive regulation of cell proliferation
regulation of smoothened signaling pathway
response to mechanical stimulus
epidermal cell differentiation
dorsal/ventral pattern formation
proximal/distal pattern formation
floor plate formation
spinal cord dorsal/ventral patterning
ventral spinal cord development
spinal cord motor neuron differentiation
cerebellar cortex morphogenesis
smoothened signaling pathway involved in ventral spinal cord interneuron specification
smoothened signaling pathway involved in spinal cord motor neuron cell fate specification
dorsal/ventral neural tube patterning
neural tube development
smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation
spinal cord ventral commissure morphogenesis
pituitary gland development
cell differentiation
lung development
mammary gland development
hindbrain development
negative regulation of chondrocyte differentiation
tube development
odontogenesis of dentin-containing tooth
embryonic digit morphogenesis
positive regulation of neuron differentiation
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic digestive tract development
developmental growth
anatomical structure formation involved in morphogenesis
neuron development
branching morphogenesis of an epithelial tube
anatomical structure development
notochord regression
head development
prostatic bud formation
mammary gland duct morphogenesis
smoothened signaling pathway involved in dorsal/ventral neural tube patterning
cellular response to organic cyclic compound
cochlea morphogenesis
cellular response to virus
transcription, DNA-templated
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support