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GLI2 Antibody (711045) in WB
Western blot analysis was performed on Nuclear enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with TGF-β1 (Serum starved and treated with 5 ng/mL for 48hr) (Lane 2), MCF-7 (Lane 3), MCF-7 treated with TGF-β1 (Serum starved and treated with 5 ng/mL for 48hr) (Lane 4), A549 (Lane 5), A549 treated with TGF-β1 (Serum starved and treated with 5 ng/mL for 48hr) (Lane 6) and tissue extracts of mouse pancreas (Lane 7), Rat Pancreas (Lane 8) and Mouse Kidney (Lane 9). The blots were probed with Anti-Gli-2 Recombinant Ra... View More
Product Details
711045
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
9HCLC
Immunogen
Protein corresponding to Human GLI2 (aa 1-332)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633108
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Bovine and Rabbit
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLI family zinc finger protein 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; oncogene GLI2; Tax helper protein; tax helper protein 1; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; Zinc finger protein GLI2
Gene Aliases: AW546128; CJS; GLI2; HPE9; PHS2; THP; THP1; THP2
UniProt ID: (Human) Q15591, (Mouse) Q0VGT2
Entrez Gene ID: (Human) 2736, (Rat) 304729, (Mouse) 14633
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
molecular_function
DNA binding
transcription regulatory region DNA binding
nucleic acid binding
metal ion binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
in utero embryonic development
kidney development
chondrocyte differentiation
osteoblast development
transcription from RNA polymerase II promoter
smoothened signaling pathway
pattern specification process
axon guidance
ventral midline development
hindgut morphogenesis
heart development
cell proliferation
regulation of smoothened signaling pathway
response to mechanical stimulus
anterior/posterior pattern specification
proximal/distal pattern formation
floor plate formation
spinal cord dorsal/ventral patterning
ventral spinal cord development
cerebellar cortex morphogenesis
smoothened signaling pathway involved in ventral spinal cord interneuron specification
smoothened signaling pathway involved in spinal cord motor neuron cell fate specification
smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation
spinal cord ventral commissure morphogenesis
pituitary gland development
lung development
mammary gland development
hindbrain development
negative regulation of chondrocyte differentiation
positive regulation of T cell differentiation in thymus
tube development
odontogenesis of dentin-containing tooth
embryonic digit morphogenesis
negative regulation of apoptotic process
positive regulation of neuron differentiation
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic digestive tract development
developmental growth
neuron development
branching morphogenesis of an epithelial tube
notochord regression
prostatic bud formation
mammary gland duct morphogenesis
smoothened signaling pathway involved in dorsal/ventral neural tube patterning
cellular response to organic cyclic compound
cochlea morphogenesis
osteoblast differentiation
morphogenesis of an epithelium
positive regulation of cell proliferation
epidermal cell differentiation
dorsal/ventral pattern formation
spinal cord motor neuron differentiation
dorsal/ventral neural tube patterning
neural tube development
cell differentiation
anatomical structure formation involved in morphogenesis
anatomical structure development
head development
cellular response to virus
transcription, DNA-templated
regulation of transcription, DNA-templated
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