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Invitrogen
GLO1 Recombinant Rabbit Monoclonal Antibody (043)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
GLO1 Antibody (MA5-31148) in WB
Western blot was performed using Anti-GLO1 Monoclonal Antibody (Product # MA5-31148) and a 21 kDa band corresponding to GLO1 was observed in Mouse Liver and Rat Liver and not in Mouse Skeletal Muscle and Mouse Spleen tissue lysates which are reported negative for GLO1 expression. Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Skeletal Muscle (Lane 2), Mouse Spleen (Lane 3) and Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a n... View More
Product Details
MA5-31148
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
043
Immunogen
Recombinant Mouse GLO1/Glyoxalase 1 protein (Ala2-Ile184)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2786798
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Mouse GLO1/Glyoxalase 1.
Target Information
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aldoketomutase; Glx I; glyoxalase 1 complex; glyoxalase 1 regulatory; glyoxalase 1 structural; Glyoxalase I; glyoxylase 1; Ketone-aldehyde mutase; Lactoylglutathione lyase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase
Gene Aliases: 0610009E22Rik; 1110008E19Rik; 2510049H23Rik; AW550643; Glo-1; Glo-1r; Glo-1s; Glo1; Glo1-r; Glo1-s; GLY1; Qglo
UniProt ID: (Rat) Q6P7Q4, (Mouse) Q9CPU0
Entrez Gene ID: (Rat) 294320, (Mouse) 109801
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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