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GLP1R Antibody (PA5-142937) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of GLP1R in HeLa cells using GLP1R Polyclonal Antibody (Product # PA5-142937). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-142937
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-QHQWDGLLSYQD.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2933579
Product Specific Information
Additional Information: The immunizing peptide represents part of an extracellular region.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
Glucagon-like peptide-1(9-36)amide and GLP-1(9-37) are the forms of GLP-1 that result from the rapid degradation of the active forms of the peptide (GLP-1(7-36)amide and GLP-1(7-37)) by the enzyme dipeptidyl peptidase-IV (DPP-IV, also known as CD26 or adenosine deaminase binding protein). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLP-1 receptor; GLP-1-R; GLP-1R; GLP-I receptor; GLP1 receptor; Glucagon-like peptide 1 receptor; glucagon-like peptide-1 receptor; pancreatic beta cell receptor for the gluco-incretin hormone glucagon-like peptide 1; seven transmembrane helix receptor
Gene Aliases: Glip; GLP-1; GLP-1-R; GLP-1R; GLP1R; Glpr; RATGL1RCP
UniProt ID: (Human) P43220, (Rat) P32301
Entrez Gene ID: (Human) 2740, (Pig) 100154059, (Rat) 25051
response to stress
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
positive regulation of cytosolic calcium ion concentration
learning or memory
regulation of heart contraction
cAMP-mediated signaling
positive regulation of blood pressure
regulation of insulin secretion
cellular response to glucagon stimulus
adenylate cyclase-activating G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
memory
feeding behavior
positive regulation of cell proliferation
associative learning
response to glucose
insulin secretion
positive regulation of insulin secretion
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of cell differentiation
positive regulation of heart contraction
positive regulation of transcription from RNA polymerase II promoter
positive regulation of glucose import
hormone secretion
release of sequestered calcium ion into cytosol
regulation of calcium ion transport
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