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FIGURE: 1 / 1
GLS2 Antibody (CF809506) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLS2 (RC212650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GLS2. (1. Positive lysates LY415701 (100 µg) and LC415701 (20 µg) can be purchased separately from OriGene.
Product Details
CF809506
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI6D11
Immunogen
Full length human recombinant protein of human GLS2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. This protein is functionally similar to the kidney glutaminase but is a little smaller in size. Originally thought to be liver-specific, this protein has been found in other tissues as well. At least one transcribed pseudogene has been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: breast cell glutaminase; GLS; glutaminase 2 (liver, mitochondrial); glutaminase GA; glutaminase I; Glutaminase liver isoform, mitochondrial; L hL L-glutaminase; L hLGA; L-glutaminase; L-glutamine amidohydrolase; LGA; liver mitochondrial glutaminase; phosphate-activated glutaminase; phosphate-dependent glutaminase
Gene Aliases: A330074B06Rik; AI195532; GA; GLS; GLS2; hLGA; Kiaa4146; LGA
UniProt ID: (Human) Q9UI32, (Rat) P28492, (Mouse) Q571F8
Entrez Gene ID: (Human) 27165, (Rat) 192268, (Mouse) 216456
cellular amino acid metabolic process
glutamate biosynthetic process
glutamine catabolic process
cellular amino acid biosynthetic process
glutamate secretion
regulation of apoptotic process
reactive oxygen species metabolic process
positive regulation of protein targeting to mitochondrion
glutamine metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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