Invitrogen

GLT-1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5 Published Figures

7 References

View all (28) GLT-1 antibodies

Datasheet

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GLT-1 Antibody (PA5-17099) in WB

Western blot was performed using Anti-GLT-1 Polyclonal Antibody, (Product # PA5-17099) and band at ~55 kDa corresponding to GLT-1 was observed in Mouse and Rat Brain and Cerebellum but not in Kidney and Lung which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Cerebellum (Lane 2), Mouse Kidney (Lane 3), Mouse Lung (Lane 4), Rat Brain (Lane 5), Rat Cerebellum (Lane 6), Rat Kidney (Lane 7) and Rat Lung (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Re... View More

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GLT-1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells owing to their inherent genetic constitution. Relative expression of GLT-1 was observed in Mouse and Rat Brain and Cerebellum and not in Kidney and Lung tissues using GLT-1 Polyclonal Antibody (Product # PA5-17099) in western blot. {RE}

Product Details

PA5-17099

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to human EAAT2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

22 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978571

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

mGlu3 Metabotropic Glutamate Receptors as a Target for the Treatment of Absence Epilepsy: Preclinical and Human Genetics Data.

Current neuropharmacology

Celli R,Striano P,Citraro R,Di Menna L,Cannella M,Imbriglio T,Koko M,De Sarro G,Monn JA,Battaglia G,Luijtelaar GV,Nicoletti F,Russo E,Leo A

Published figure using GLT-1 polyclonal antibody (Product # PA5-17099) in Western Blot

Fri Feb 03 00:00:00 EST 2023

Mature iPSC-derived astrocytes of an ALS/FTD patient carrying the TDP43 mutation display a mild reactive state and release polyP toxic to motoneurons.

Frontiers in cell and developmental biology

Rojas F,Aguilar R,Almeida S,Fritz E,Corvalán D,Ampuero E,Abarzúa S,Garcés P,Amaro A,Diaz I,Arredondo C,Cortes N,Sanchez M,Mercado C,Varela-Nallar L,Gao FB,Montecino M,van Zundert B

PA5-17099 was used in Immunocytochemistry to establish that human astrocytes carrying the TDP43A90V mutation exhibit a cell-autonomous pathological signature, hence providing an experimental model to decipher the molecular mechanisms underlying the generation of the neurotoxic phenotype.

Sat Mar 23 00:00:00 EDT 2024

Recurrent SLC1A2 variants cause epilepsy via a dominant negative mechanism.

Annals of neurology

Stergachis AB,Pujol-Giménez J,Gyimesi G,Fuster D,Albano G,Troxler M,Picker J,Rosenberg PA,Bergin A,Peters J,El Achkar CM,Harini C,Manzi S,Rotenberg A,Hediger MA,Rodan LH

PA5-17099 was used in Western Blotting to demonstrate that treatment of a 20-month-old SLC1A2-related epilepsy patient with the SLC1A2-modulating agent ceftriaxone did not result in a significant change in daily spasm count.

Sat Jun 01 00:00:00 EDT 2019

The Hydroxyl Side Chain of a Highly Conserved Serine Residue Is Required for Cation Selectivity and Substrate Transport in the Glial Glutamate Transporter GLT-1/SLC1A2.

The Journal of biological chemistry

Simonin A,Montalbetti N,Gyimesi G,Pujol-Giménez J,Hediger MA

PA5-17099 was used in western blot to study the importance of serine 364 on glutamate transporter function

Fri Dec 18 00:00:00 EST 2015

The Hydroxyl Side Chain of a Highly Conserved Serine Residue Is Required for Cation Selectivity and Substrate Transport in the Glial Glutamate Transporter GLT-1/SLC1A2.

The Journal of biological chemistry

Simonin A,Montalbetti N,Gyimesi G,Pujol-Giménez J,Hediger MA

PA5-17099 was used in western blot to study the importance of serine 364 on glutamate transporter function

Fri Dec 18 00:00:00 EST 2015

The Hydroxyl Side Chain of a Highly Conserved Serine Residue Is Required for Cation Selectivity and Substrate Transport in the Glial Glutamate Transporter GLT-1/SLC1A2.

The Journal of biological chemistry

Simonin A,Montalbetti N,Gyimesi G,Pujol-Giménez J,Hediger MA

PA5-17099 was used in western blot to study the importance of serine 364 on glutamate transporter function

Fri Dec 18 00:00:00 EST 2015

HFE polymorphisms affect cellular glutamate regulation.

Neurobiology of aging

Mitchell RM,Lee SY,Simmons Z,Connor JR

PA5-17099 was used in western blot to examine the role of HFE polymorphisms in neurodegenerative diseases

Wed Jun 01 00:00:00 EDT 2011

Functional expression, purification and high sequence coverage mass spectrometric characterization of human excitatory amino acid transporter EAAT2.

Protein expression and purification

Ye R,Rhoderick JF,Thompson CM,Bridges RJ

PA5-17099 was used in western blot to investigate a new efficient approach to produce and characterize human excitatory amino acid transporter 2

Mon Nov 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: EAAT2 variant mEAAT2/5UT5; Excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1a; GLT1b; GLT1c; GLUT-R; glutamate transporter 1c; Glutamate/aspartate transporter II; high affinity glucose transporter; OTTHUMP00000165209; OTTHUMP00000165210; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2; solute carrier family 1, member 2

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Gene Aliases: 1700091C19Rik; 2900019G14Rik; AI159670; EAAT2; Glt; GLT-1; GLT1; HBGT; MGLT1; SLC1A2

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UniProt ID: (Human) P43004, (Mouse) P43006, (Rat) P31596

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Entrez Gene ID: (Human) 6506, (Mouse) 20511, (Rat) 29482

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Function(s)

L-glutamate transmembrane transporter activity high-affinity glutamate transmembrane transporter activity protein binding amino acid transmembrane transporter activity glutamate:sodium symporter activity symporter activity sodium:dicarboxylate symporter activity primary active transporter

Process(es)

amino acid transmembrane transport ion transport chemical synaptic transmission visual behavior response to wounding multicellular organism aging glutamate secretion telencephalon development adult behavior cellular response to extracellular stimulus multicellular organism growth response to drug response to amino acid positive regulation of glucose import L-glutamate import D-aspartate import L-glutamate transmembrane transport transport dicarboxylic acid transport nervous system development response to light stimulus L-glutamate transport sodium ion transmembrane transport neurotransmitter transport

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GLT-1 Polyclonal Antibody

GLT-1 Antibody (PA5-17099) in WB

Product Details

PA5-17099

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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