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Invitrogen
GLT-1 Recombinant Superclonal Antibody (9 HCLC)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
GLT-1 Antibody (711020) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized U-87 MG cells for detection of endogenous EAAT2 using Anti-EAAT2 Recombinant Rabbit Polyclonal Antibody (Product # 711020, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EAAT2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S369... View More
Product Details
711020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
9 HCLC
Immunogen
Peptides corresponding to Human SLC1A2 (aa 5-20, 148-166)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633106
Product Specific Information
This antibody is predicted to react with Monkey, Sheep and Cat.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EAAT2 variant mEAAT2/5UT5; Excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1a; GLT1b; GLT1c; GLUT-R; glutamate transporter 1c; Glutamate/aspartate transporter II; high affinity glucose transporter; OTTHUMP00000165209; OTTHUMP00000165210; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2; solute carrier family 1, member 2
Gene Aliases: 1700091C19Rik; 2900019G14Rik; AI159670; EAAT2; Glt; GLT-1; GLT1; HBGT; MGLT1; SLC1A2
UniProt ID: (Human) P43004, (Mouse) P43006, (Rat) P31596
Entrez Gene ID: (Human) 6506, (Mouse) 20511, (Rat) 29482
amino acid transmembrane transport
ion transport
chemical synaptic transmission
visual behavior
response to wounding
multicellular organism aging
glutamate secretion
telencephalon development
adult behavior
cellular response to extracellular stimulus
multicellular organism growth
response to drug
response to amino acid
positive regulation of glucose import
L-glutamate import
D-aspartate import
L-glutamate transmembrane transport
transport
dicarboxylic acid transport
nervous system development
response to light stimulus
L-glutamate transport
sodium ion transmembrane transport
neurotransmitter transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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