Invitrogen

GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

5 References

View all (39) GLUT1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 16

GLUT1 Antibody (MA5-31960) in Flow

Flow Cytometric analysis of GLUT1 in Hela cells using a GLUT1 Monoclonal Antibody (Product # MA5-31960) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

GLUT1 Antibody in Immunocytochemistry (ICC/IF)

GLUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

GLUT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with GLUT1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377) (Product # MA5-31960). GLUT-1 shows a streak like pattern in cell lines as seen in all the 3 lanes. The siRNA Lane shows almost 90% loss of streak like signal. {KD}

Product Details

MA5-31960

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:5,000

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:500

Flow Cytometry (Flow)

1:500-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SA0377

Immunogen

Synthetic peptide within Human GLUT1 aa 443-492

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809254

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sphingosine-1-phosphate-lyase deficiency affects glucose metabolism in a way that abets oncogenesis.

Molecular oncology

Afsar SY,Alam S,Fernandez Gonzalez C,van Echten-Deckert G

MA5-31960 was used in Western Blotting to report on a newly identified role of the S1P/S1PR1-3 axis in glucose metabolism in SGPL1-deficient mouse embryonic fibroblasts.

Sat Oct 01 00:00:00 EDT 2022

Sphingosine-1-phosphate-lyase deficiency affects glucose metabolism in a way that abets oncogenesis.

Molecular oncology

Afsar SY,Alam S,Fernandez Gonzalez C,van Echten-Deckert G

MA5-31960 was used in Western Blotting to report on a newly identified role of the S1P/S1PR1-3 axis in glucose metabolism in SGPL1-deficient mouse embryonic fibroblasts.

Sat Oct 01 00:00:00 EDT 2022

Activation of Wnt/β-catenin pathway mitigates blood-brain barrier dysfunction in Alzheimer's disease.

Brain : a journal of neurology

Wang Q,Huang X,Su Y,Yin G,Wang S,Yu B,Li H,Qi J,Chen H,Zeng W,Zhang K,Verkhratsky A,Niu J,Yi C

MA5-31960 was used in Immunohistochemistry-immunofluorescence to show dysfunctional blood-brain barrier in patients with Alzheimer's disease reflected by perivascular accumulation of blood-derived fibrinogen in the hippocampus and cortex, accompanied by decreased tight junction proteins Claudin-5 and glucose transporter Glut-1 in the brain endothelial cells.

Mon Dec 19 00:00:00 EST 2022

Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target.

Nature communications

Cho H,Kwon HY,Sharma A,Lee SH,Liu X,Miyamoto N,Kim JJ,Im SH,Kang NY,Chang YT

MA5-31960 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot, Immunohistochemistry (Frozen) to demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

Mon Oct 10 00:00:00 EDT 2022

The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers.

Scientific reports

Risha Y,Minic Z,Ghobadloo SM,Berezovski MV

MA5-31960 was used in Flow cytometry/Cell sorting to demonstrate that exosomes are a rich source of breast cancer-related proteins and surface biomarkers that may be used for disease diagnosis and prognosis.

Tue Aug 11 00:00:00 EDT 2020

The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers.

Scientific reports

Risha Y,Minic Z,Ghobadloo SM,Berezovski MV

Tue Aug 11 00:00:00 EDT 2020

Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa.

Cell reports

Wang W,Kini A,Wang Y,Liu T,Chen Y,Vukmanic E,Emery D,Liu Y,Lu X,Jin L,Lee SJ,Scott P,Liu X,Dean K,Lu Q,Fortuny E,James R,Kaplan HJ,Du J,Dean DC

MA5-31960 was used in Immunohistochemistry-immunofluorescence to provide evidence that contact between externalized phosphatidylserine (PS) on OS tips and apical RPE receptors activates Akt, linking phagocytosis with glucose transport to photoreceptors for new OS synthesis.

Tue Jul 30 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); glucose transporter glut1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1; HepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; MGC141895; MGC141896; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Solute carrier family 2 a 1 (facilitated glucose transporter) brain; Solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, member 1

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Gene Aliases: CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; GLUTB; GTG1; Gtg3; HTLVR; PED; RATGTG1; SDCHCN; SLC2A1

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UniProt ID: (Human) P11166, (Mouse) P17809, (Rat) P11167

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Entrez Gene ID: (Human) 6513, (Mouse) 20525, (Rat) 24778

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Function(s)

glucose transmembrane transporter activity protein binding kinase binding dehydroascorbic acid transporter activity identical protein binding xenobiotic transporter activity protein self-association D-glucose transmembrane transporter activity transporter activity sugar:proton symporter activity transmembrane transporter activity substrate-specific transmembrane transporter activity

Process(es)

protein complex assembly transport carbohydrate transport glucose transport proton transport hexose transmembrane transport cellular response to glucose starvation xenobiotic transport glucose import transmembrane transport dehydroascorbic acid transport cation transmembrane transport lactose biosynthetic process response to osmotic stress L-ascorbic acid metabolic process regulation of insulin secretion glucose transmembrane transport

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377)

GLUT1 Antibody (MA5-31960) in Flow

Product Details

MA5-31960

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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