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GM-CSF Antibody (500-P225BT-25UG) in ELISA
Sandwich ELISA: To detect Rat GM-CSF by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 µg/mL of GM-CSF Polyclonal Antibody, Biotin (Product # 500-P225BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech GM-CSF Polyclonal Antibody (Product # 500-P225-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat GM-CSF.
Product Details
500-P225BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat GM-CSF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929904
Product Specific Information
AA Sequence of recombinant protein: MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat GM-CSF. Anti-Rat GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat GM-CSF by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Rat GM-CSF (500-P225) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat GM-CSF.
Western Blot: To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Rat GM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P225BT-1MG will be provided as 2 x 500 µg
Target Information
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257
Gene Aliases: Csf2; Csfgm; Gm-csf; Gmcsf
UniProt ID: (Rat) P48750
Entrez Gene ID: (Rat) 116630
embryonic placenta development
immune response
positive regulation of cell proliferation
regulation of gene expression
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of interleukin-23 production
epithelial fluid transport
macrophage activation
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
myeloid dendritic cell differentiation
positive regulation of macrophage differentiation
positive regulation of DNA replication
negative regulation of cytolysis
epithelial cell proliferation
cellular response to lipopolysaccharide
positive regulation of podosome assembly
dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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