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Invitrogen
GM-CSF Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
GM-CSF Antibody (PA5-47982) in Neu
Neutralization of GM-CSF in TF‚1 human erythroleukemic cell line. Samples were incubated in GM-CSF polyclonal antibody (Product # PA5-47982) using a dilution of 50 ng/mL. Recombinant Feline GM‚CSF stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline GM‚CSF is neutralized (green line) by increasing concentrations of Goat Anti‚Feline GM‚CSF Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.5-2.0 µg/mL.
Product Details
PA5-47982
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant feline GM-CSF
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610127
Product Specific Information
In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse GM-CSF, recombinant rat GM-CSF, and recombinant human GM-CSF is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257
Gene Aliases: CSF2; GM-CSF
UniProt ID: (Cat) O62757
Entrez Gene ID: (Cat) 493805
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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