Invitrogen

GM-CSF Monoclonal Antibody (1089)

Product Details

M501

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1089

Immunogen

Glycosylated, CHO-derived recombinant human GM-CSF

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

RRID

AB_223571

Product Specific Information

M501 targets Human GM-CSF in ELISA applications and shows reactivity with Human samples.

The M501 immunogen is glycosylated, CHO-derived recombinant human GM-CSF.

M501 detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.

Blood

Burke SM,Issekutz TB,Mohan K,Lee PW,Shmulevitz M,Marshall JS

M501 was used in ELISA to study the role of human mast cells in the recruitment of natural killer cells

Sun Jun 15 00:00:00 EDT 2008

Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women.

International archives of allergy and immunology

Tsunoda M,Litonjua AA,Kuniak MP,Weiss ST,Satoh T,Guevarra L,Tollerud DJ

M501 was used in ELISA to investigate the relationship between serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women

Sat Feb 01 00:00:00 EST 2003

Transgenic overexpression of human IL-17E results in eosinophilia, B-lymphocyte hyperplasia, and altered antibody production.

Blood

Kim MR,Manoukian R,Yeh R,Silbiger SM,Danilenko DM,Scully S,Sun J,DeRose ML,Stolina M,Chang D,Van GY,Clarkin K,Nguyen HQ,Yu YB,Jing S,Senaldi G,Elliott G,Medlock ES

M501 was used in ELISA to investigate the effect of human interleukin 17E overexpression in transgenic mice

Tue Oct 01 00:00:00 EDT 2002

The Trypanosoma cruzi trans-sialidase is a T cell-independent B cell mitogen and an inducer of non-specific Ig secretion.

International immunology

Gao W,Wortis HH,Pereira MA

M501 was used in ELISA to study the role of Trypanosoma cruzi trans-sialidase in B cell proliferation and Ig secretion

Fri Mar 01 00:00:00 EST 2002

The effect of T1 and T2 cytokines on the cytotoxic T cell response to mannan-MUC1.

Cancer immunology, immunotherapy : CII

Lees CJ,Apostolopoulos V,Acres B,Ong CS,Popovski V,McKenzie IF

M501 was used in ELISA to investigate the effect of T1 and T2 cytokines on the cytotoxic T cell response to mannan-MUC1

Tue Feb 01 00:00:00 EST 2000

A mouse thymic stromal cell line producing macrophage-colony stimulating factor and interleukin-6.

Archives of pharmacal research

Lee CK,Kim JK,Kim K,Han SS

M501 was used in ELISA to detect M-CSF and interleukin 6 in a mouse thymic stromal cell line

Thu Jun 01 00:00:00 EDT 2000

Antitumor efficacy of adenocarcinoma cells engineered to produce interleukin 12 (IL-12) or other cytokines compared with exogenous IL-12.

Journal of the National Cancer Institute

Cavallo F,Signorelli P,Giovarelli M,Musiani P,Modesti A,Brunda MJ,Colombo MP,Forni G

M501 was used in ELISA to investigate the antitumor efficacy of adenocarcinoma cells engineered to produce interleukin 12 or other cytokines

Wed Jul 16 00:00:00 EDT 1997

The IgV domain of human B7-2 (CD86) is sufficient to co-stimulate T lymphocytes and induce cytokine secretion.

International immunology

Rennert P,Furlong K,Jellis C,Greenfield E,Freeman GJ,Ueda Y,Levine B,June CH,Gray GS

M501 was used in ELISA to investigate the role of the IgV domain of human B7-2 in T lymphocyte stimulation and cytokine secretion

Sun Jun 01 00:00:00 EDT 1997

Local and systemic response of mice to interferon-alpha 1-transfected Friend leukemia cells.

The American journal of pathology

Gabriele L,Kaido T,Woodrow D,Moss J,Ferrantini M,Proletti E,Santodonato L,Rozera C,Maury C,Belardelli F

M501 was used in ELISA to investigate the effect of injection of interferon-alpha 1-transfected Friend leukemia cells in mice

Tue Aug 01 00:00:00 EDT 1995

Bioinformatics

Protein Aliases: colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257; Molgramostin; Sargramostim

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Gene Aliases: CSF2; GMCSF

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UniProt ID: (Human) P04141

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Entrez Gene ID: (Human) 1437

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Function(s)

protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity cytokine activity granulocyte macrophage colony-stimulating factor receptor binding protein binding growth factor activity cytokine

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GM-CSF Monoclonal Antibody (1089)

Product Details

M501

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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