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GM130 Antibody (PA1-077-A555) in ICC/IF
Immunofluorescent analysis of GM-130 (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a GM-130 Polyclonal Antibody, AlexaFluor 555 conjugate (Product # PA1-077-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer.... View More
Product Details
PA1-077-A555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues G(368) Q V M E S V R Q L Q M E R D K(383) of human GM130
Conjugate
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2663178
Product Specific Information
PA1077A555 detects GM130 from human samples.
The PA1077A555 immunogen is a synthetic peptide corresponding to residues G(368) Q V M E S V R Q L Q M E R D K(383) of human GM130.
Target Information
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; golgi autoantigen, golgin subfamily a, 2; Golgi matrix protein GM130; Golgin subfamily A member 2; Golgin-95; RP11-395P17.5; SY11 protein
Gene Aliases: GM130; GOLGA2
UniProt ID: (Human) Q08379
Entrez Gene ID: (Human) 2801
protein glycosylation
ER to Golgi vesicle-mediated transport
microtubule nucleation
asymmetric cell division
negative regulation of autophagy
negative regulation of protein binding
COPII vesicle coating
spindle assembly
protein homotetramerization
centrosome organization
positive regulation of protein glycosylation
Golgi ribbon formation
Golgi disassembly
spindle assembly involved in meiosis
mitotic spindle assembly
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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