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GM130 Antibody (MA5-50784) in IHC (P)
Immunohistochemistry of GM130 in paraffin-embedded mouse kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with GM130 recombinant monoclonal antibody (Product # MA5-50784) with a dilution of 1:1,000 for 1 hour at room ... View More
Product Details
MA5-50784
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE42-53
Immunogen
Recombinant protein within human GM130 aa 1-100/1002 (N terminal).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3094236
Product Specific Information
Positive control: MCF-7 cell lysates, mouse kidney tissue, rat kidney tissue.
Predicted band size: 113 kDa
Subcellular Location: Cytoplasm, Cytoskeleton, Membrane, Microtubule.
Target Information
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa cis-Golgi matrix protein; cis-Golgi matrix protein GM130; GM130; GM130 autoantigen; golgi autoantigen, golgin subfamily a, 2; Golgi matrix protein GM130; Golgin subfamily A member 2; Golgin-95; RP11-395P17.5; SY11 protein
Gene Aliases: AW555139; GM130; GOLGA2; mKIAA4150
UniProt ID: (Human) Q08379, (Rat) Q62839, (Mouse) Q921M4
Entrez Gene ID: (Human) 2801, (Rat) 64528, (Mouse) 99412
protein glycosylation
ER to Golgi vesicle-mediated transport
microtubule nucleation
asymmetric cell division
negative regulation of autophagy
negative regulation of protein binding
COPII vesicle coating
spindle assembly
protein homotetramerization
centrosome organization
positive regulation of protein glycosylation
Golgi ribbon formation
Golgi disassembly
spindle assembly involved in meiosis
mitotic spindle assembly
cell-cell adhesion
biological_process
positive regulation of axonogenesis
Golgi localization
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