Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
GNAI1 Antibody (PA5-28223) in IHC (P)
GNAI1 Polyclonal Antibody detects GNAI1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GNAI1 Polyclonal Antibody (Product # PA5-28223) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-28223
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 23 and 354 of Human G protein alpha inhibitor 1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545699
Product Specific Information
Recommended positive controls: A431, HeLaS3.
Predicted reactivity: Mouse (100%), Rat (99%), Zebrafish (96%), Xenopus laevis (97%), Pig (100%), Chicken (97%), Bovine (100%), Guinea pig (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain; and transducin-1 and transducin-2, proteins involved in phototransduction in retinal rods and cones, respectively.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Adenylate cyclase-inhibiting G alpha protein; Gi1 protein alpha subunit; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1; Guanine nucleotide-binding protein G(i) subunit alpha-1
Gene Aliases: Gi; GNAI1
UniProt ID: (Human) P63096
Entrez Gene ID: (Human) 2770
protein folding
cell cycle
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
response to peptide hormone
regulation of cAMP-mediated signaling
negative regulation of synaptic transmission
cell division
cellular response to forskolin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support