Proteintech

GNPAT Polyclonal Antibody

View all (16) GNPAT antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (10)

GNPAT Antibody in Immunocytochemistry (ICC/IF)

GNPAT Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

GNPAT Antibody (14931-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using GNPAT antibody 14931-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GNPAT Antibody in Immunocytochemistry (ICC/IF)

GNPAT Antibody in Western Blot (WB)

GNPAT Antibody in Western Blot (WB)

GNPAT Antibody in Western Blot (WB)

GNPAT Antibody in Western Blot (WB)

GNPAT Antibody in Western Blot (WB)

GNPAT Antibody in Western Blot (WB)

GNPAT Antibody in Western Blot (WB)

GNPAT Antibody in Western Blot (WB)

GNPAT Antibody in Immunoprecipitation (IP)

Product Image Gallery

GNPAT Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using GNPAT antibody 14931-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GNPAT Antibody in Western Blot (WB) — Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GNPAT Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

GNPAT Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

GNPAT Antibody in Western Blot (WB) — COLO 320 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GNPAT Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP ( GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

GNPAT Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

GNPAT Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

GNPAT Antibody in Western Blot (WB) — WB result of GNPAT antibody (14931-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNPAT transfected HepG2 cells.

GNPAT Antibody in Immunoprecipitation (IP) — IP result of anti-GNPAT (IP:14931-1-AP, 4ug; Detection:14931-1-AP 1:500) with COLO 320 cells lysate 2400ug.

Product Details

14931-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GNPAT Fusion Protein Ag6735 (331-680 aa encoded by BC000450)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognize the 69 kDa GNPAT protein.

Immunogen sequence: PVSLRSLAA GRMSRSSYNL VPRYIPQKQS EDMHAFVTEV AYKMELLQIE NMVLSPWTLI VAVLLQNRPS MDFDALVEKT LWLKGLTQAF GGFLIWPDNK PAEEVVPASI LLHSNIASLV KDQVILKVDS GDSEVVDGLM LQHITLLMCS AYRNQLLNIF VRPSLVAVAL QMTPGFRKED VYSCFRFLRD VFADEFIFLP GNTLKDFEEG CYLLCKSEAI QVTTKDILVT EKGNTVLEFL VGLFKPFVES YQIICKHLLS EEEDHFSEEQ YLAAVRKFTS QLLDQGTSQC YDVLSSDVQK NALAACVRLG VVEKKKINNN CIFNVNEPAT TKLEEMLGCK TPIGKPATAK L (331-680 aa encoded by BC000450)

Target Information

GNPAT is a key enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in GNPAT are associated with rhizomelic chondrodysplasia punctata type 2, which is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ether phospholipids are required for mitochondrial reactive oxygen species homeostasis.

Nature communications

Chen Z,Ho IL,Soeung M,Yen EY,Liu J,Yan L,Rose JL,Srinivasan S,Jiang S,Edward Chang Q,Feng N,Gay JP,Wang Q,Wang J,Lorenzi PL,Veillon LJ,Wei B,Weinstein JN,Deem AK,Gao S,Genovese G,Viale A,Yao W,Lyssiotis CA,Marszalek JR,Draetta GF,Ying H

Mon Apr 17 00:00:00 EDT 2023

Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.

Frontiers in cell and developmental biology

Karadayi R,Mazzocco J,Leclere L,Buteau B,Gregoire S,Belloir C,Koudsi M,Bessard P,Bizeau JB,Dubus E,Fenech C,Briand L,Bretillon L,Bron AM,Fioramonti X,Acar N

Thu May 12 00:00:00 EDT 2022

Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.

Frontiers in cell and developmental biology

Karadayi R,Mazzocco J,Leclere L,Buteau B,Gregoire S,Belloir C,Koudsi M,Bessard P,Bizeau JB,Dubus E,Fenech C,Briand L,Bretillon L,Bron AM,Fioramonti X,Acar N

Thu May 12 00:00:00 EDT 2022

Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.

Frontiers in cell and developmental biology

Karadayi R,Mazzocco J,Leclere L,Buteau B,Gregoire S,Belloir C,Koudsi M,Bessard P,Bizeau JB,Dubus E,Fenech C,Briand L,Bretillon L,Bron AM,Fioramonti X,Acar N

Thu May 12 00:00:00 EDT 2022

Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.

Frontiers in cell and developmental biology

Karadayi R,Mazzocco J,Leclere L,Buteau B,Gregoire S,Belloir C,Koudsi M,Bessard P,Bizeau JB,Dubus E,Fenech C,Briand L,Bretillon L,Bron AM,Fioramonti X,Acar N

Thu May 12 00:00:00 EDT 2022

Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.

Frontiers in cell and developmental biology

Karadayi R,Mazzocco J,Leclere L,Buteau B,Gregoire S,Belloir C,Koudsi M,Bessard P,Bizeau JB,Dubus E,Fenech C,Briand L,Bretillon L,Bron AM,Fioramonti X,Acar N

Thu May 12 00:00:00 EDT 2022

Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.

Frontiers in cell and developmental biology

Karadayi R,Mazzocco J,Leclere L,Buteau B,Gregoire S,Belloir C,Koudsi M,Bessard P,Bizeau JB,Dubus E,Fenech C,Briand L,Bretillon L,Bron AM,Fioramonti X,Acar N

Thu May 12 00:00:00 EDT 2022

Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors.

International journal of molecular sciences

Meyer MT,Watermann C,Dreyer T,Wagner S,Wittekindt C,Klussmann JP,Ergün S,Baumgart-Vogt E,Karnati S

Fri Jul 23 00:00:00 EDT 2021

Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.

The Journal of clinical investigation

Park H,He A,Tan M,Johnson JM,Dean JM,Pietka TA,Chen Y,Zhang X,Hsu FF,Razani B,Funai K,Lodhi IJ

Fri Feb 01 00:00:00 EST 2019

Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.

The Journal of clinical investigation

Park H,He A,Tan M,Johnson JM,Dean JM,Pietka TA,Chen Y,Zhang X,Hsu FF,Razani B,Funai K,Lodhi IJ

Fri Feb 01 00:00:00 EST 2019

Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.

Cell host & microbe

Jean Beltran PM,Cook KC,Hashimoto Y,Galitzine C,Murray LA,Vitek O,Cristea IM

Wed Oct 10 00:00:00 EDT 2018

Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.

Cell host & microbe

Jean Beltran PM,Cook KC,Hashimoto Y,Galitzine C,Murray LA,Vitek O,Cristea IM

Wed Oct 10 00:00:00 EDT 2018

Differential activities of peroxisomes along the mouse intestinal epithelium.

Cell biochemistry and function

Morvay PL,Baes M,Van Veldhoven PP

Sat Apr 01 00:00:00 EDT 2017

Differential activities of peroxisomes along the mouse intestinal epithelium.

Cell biochemistry and function

Morvay PL,Baes M,Van Veldhoven PP

Sat Apr 01 00:00:00 EDT 2017

C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism.

Histochemistry and cell biology

Karnati S,Palaniswamy S,Alam MR,Oruqaj G,Stamme C,Baumgart-Vogt E

Tue Mar 01 00:00:00 EST 2016

C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism.

Histochemistry and cell biology

Karnati S,Palaniswamy S,Alam MR,Oruqaj G,Stamme C,Baumgart-Vogt E

Tue Mar 01 00:00:00 EST 2016

Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3.

Human mutation

Itzkovitz B,Jiralerspong S,Nimmo G,Loscalzo M,Horovitz DD,Snowden A,Moser A,Steinberg S,Braverman N

Sun Jan 01 00:00:00 EST 2012

Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3.

Human mutation

Itzkovitz B,Jiralerspong S,Nimmo G,Loscalzo M,Horovitz DD,Snowden A,Moser A,Steinberg S,Braverman N

Sun Jan 01 00:00:00 EST 2012

Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3.

Human mutation

Itzkovitz B,Jiralerspong S,Nimmo G,Loscalzo M,Horovitz DD,Snowden A,Moser A,Steinberg S,Braverman N

Sun Jan 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: acyl-CoA:dihydroxyacetonephosphate acyltransferase; Acyl-CoA:dihydroxyacetonephosphateacyltransferase; DAP-AT; DHAP-AT; Dihydroxyacetone phosphate acyltransferase; Glycerone-phosphate O-acyltransferase

View more View less

Gene Aliases: AU019525; D1Ertd819e; DAP-AT; DAPAT; DHAPAT; GNPAT; RCDP2

View more View less

UniProt ID: (Human) O15228, (Mouse) P98192, (Rat) Q9ES71

View more View less

Entrez Gene ID: (Human) 8443, (Mouse) 14712, (Rat) 84470

View more View less

receptor binding glycerone-phosphate O-acyltransferase activity palmitoyl-CoA hydrolase activity catalytic activity protein binding O-acyltransferase activity transferase activity transferase activity, transferring acyl groups acyltransferase

Process(es)

glycerophospholipid metabolic process phosphatidic acid biosynthetic process synapse assembly response to nutrient ether lipid biosynthetic process cerebellum morphogenesis paranodal junction assembly response to drug response to starvation membrane organization response to fatty acid metabolic process myelination cellular lipid metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support