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GOLGA1 Antibody (PA5-115309) in ICC/IF
Immunocytochemistry analysis of GOLGA1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115309) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115309
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GOLGA1(Accession Q92805), corresponding to amino acid residues E729-S767.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899945
Product Specific Information
Antibody detects endogenous levels of total Golgin 97.
Target Information
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The Golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gap junction protein, alpha 4, 37kD; golgi autoantigen, golgin subfamily a, 1; Golgin subfamily A member 1; Golgin-97
Gene Aliases: 0710001G09Rik; 2210418B03Rik; AW107649; GOLGA1; Golgi97; golgin-97
UniProt ID: (Human) Q92805, (Mouse) Q9CW79
Entrez Gene ID: (Human) 2800, (Mouse) 76899, (Rat) 311919
Performance Guarantee
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