Proteintech

GOT1 Polyclonal Antibody

GOT1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

GOT1 Antibody (14886-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed L02 cells using 14886-1-AP (GOT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

GOT1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14886-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 29 publications 29 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GOT1 Fusion Protein Ag6671 (1-413 aa encoded by BC000498)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.49 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAPPSVFAE VPQAQPVLVF KLTADFREDP DPRKVNLGVG AYRTDDCHPW VLPVVKKVEQ KIANDNSLNH EYLPILGLAE FRSCASRLAL GDDSPALKEK RVGGVQSLGG TGALRIGADF LARWYNGTNN KNTPVYVSSP TWENHNAVFS AAGFKDIRSY RYWDAEKRGL DLQGFLNDLE NAPEFSIVVL HACAHNPTGI DPTPEQWKQI ASVMKHRFLF PFFDSAYQGF ASGNLERDAW AIRYFVSEGF EFFCAQSFSK NFGLYNERVG NLTVVGKEPE SILQVLSQME KIVRITWSNP PAQGARIVAS TLSNPELFEE WTGNVKTMAD RILTMRSELR ARLEALKTPG TWNHITDQIG MFSFTGLNPK QVEYLVNEKH IYLLPSGRIN VSGLTTKNLD YVATSIHEAV TKIQ (1-413 aa encoded by BC000498)

Target Information

GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation.

Frontiers in microbiology

Li F,Fu X,Luo X,Lin Q,Liang H,Niu Y,Liu L,Li N

Tue Apr 18 00:00:00 EDT 2023

ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells.

Cancer research

Hu Q,Dai J,Zhang Z,Yu H,Zhang J,Zhu X,Qin Y,Zhang L,Zhang P

Mon May 15 00:00:00 EDT 2023

Metabolic clearance of oxaloacetate and mitochondrial complex II respiration: Divergent control in skeletal muscle and brown adipose tissue.

Biochimica et biophysica acta. Bioenergetics

Yu L,Fink BD,Som R,Rauckhorst AJ,Taylor EB,Sivitz WI

Sun Jan 01 00:00:00 EST 2023

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

EMBO molecular medicine

Southwell N,Primiano G,Nadkarni V,Attarwala N,Beattie E,Miller D,Alam S,Liparulo I,Shurubor YI,Valentino ML,Carelli V,Servidei S,Gross SS,Manfredi G,Chen Q,D'Aurelio M

Mon Jul 10 00:00:00 EDT 2023

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Cell

Mo X,Niu Q,Ivanov AA,Tsang YH,Tang C,Shu C,Li Q,Qian K,Wahafu A,Doyle SP,Cicka D,Yang X,Fan D,Reyna MA,Cooper LAD,Moreno CS,Zhou W,Owonikoko TK,Lonial S,Khuri FR,Du Y,Ramalingam SS,Mills GB,Fu H

Thu May 26 00:00:00 EDT 2022

Gaseous signal molecule SO₂ regulates autophagy through PI3K/AKT pathway inhibits cardiomyocyte apoptosis and improves myocardial fibrosis in rats with type II diabetes.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Zhao J,Wu Q,Yang T,Nie L,Liu S,Zhou J,Chen J,Jiang Z,Xiao T,Yang J,Chu C

Tue Nov 01 00:00:00 EDT 2022

Glutamine synthetase limits β-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1.

The Journal of clinical investigation

Dai W,Shen J,Yan J,Bott AJ,Maimouni S,Daguplo HQ,Wang Y,Khayati K,Guo JY,Zhang L,Wang Y,Valvezan A,Ding WX,Chen X,Su X,Gao S,Zong WX

Thu Dec 15 00:00:00 EST 2022

A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H₂S oxidation.

The Journal of biological chemistry

Kumar R,Landry AP,Guha A,Vitvitsky V,Lee HJ,Seike K,Reddy P,Lyssiotis CA,Banerjee R

Sat Jan 01 00:00:00 EST 2022

Loss of Heterozygosity for Promotes Malignant Phenotype of Pancreatic Ductal Adenocarcinoma by Activating HIF-2α-c-Myc-Regulated Glutamine Metabolism.

International journal of molecular sciences

Ma Y,Ling S,Li Y,Hu M,Kong B,Huang P,Liu H

Wed Jun 15 00:00:00 EDT 2022

Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells.

International journal of oncology

Terado T,Kim CJ,Ushio A,Minami K,Tambe Y,Kageyama S,Kawauchi A,Tsunoda T,Shirasawa S,Tanaka H,Inoue H

Thu Sep 01 00:00:00 EDT 2022

Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.

Journal of hazardous materials

Zhu Y,Zhang Y,Li Y,Guo C,Fan Z,Li Y,Yang M,Zhou X,Sun Z,Wang J

Fri Jul 15 00:00:00 EDT 2022

The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis.

The Journal of biological chemistry

Vitvitsky V,Kumar R,Libiad M,Maebius A,Landry AP,Banerjee R

Tue Aug 31 00:00:00 EDT 2021

Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis.

Pharmacological research

Wang Y,Guan X,Gao CL,Ruan W,Zhao S,Kai G,Li F,Pang T

Thu Jul 01 00:00:00 EDT 2021

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

The Journal of experimental medicine

Liu M,Wang Y,Yang C,Ruan Y,Bai C,Chu Q,Cui Y,Chen C,Ying G,Li B

Mon Mar 02 00:00:00 EST 2020

Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.

Mediators of inflammation

Wang S,Sun Y,Bai Y,Zhou N,Chen N,Miller EJ,Zhang Y,Li W

Wed Nov 03 00:00:00 EDT 2021

Ischemic Neuroprotectant PKCε Restores Mitochondrial Glutamate Oxaloacetate Transaminase in the Neuronal NADH Shuttle after Ischemic Injury.

Translational stroke research

Xu J,Khoury N,Jackson CW,Escobar I,Stegelmann SD,Dave KR,Perez-Pinzon MA

Mon Jun 01 00:00:00 EDT 2020

Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

Cell reports

Bott AJ,Shen J,Tonelli C,Zhan L,Sivaram N,Jiang YP,Yu X,Bhatt V,Chiles E,Zhong H,Maimouni S,Dai W,Velasquez S,Pan JA,Muthalagu N,Morton J,Anthony TG,Feng H,Lamers WH,Murphy DJ,Guo JY,Jin J,Crawford HC,Zhang L,White E,Lin RZ,Su X,Tuveson DA,Zong WX

Tue Oct 29 00:00:00 EDT 2019

Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.

Nature communications

Wang Y,Bai C,Ruan Y,Liu M,Chu Q,Qiu L,Yang C,Li B

Mon Jan 14 00:00:00 EST 2019

Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.

Nature communications

Wang Y,Bai C,Ruan Y,Liu M,Chu Q,Qiu L,Yang C,Li B

Mon Jan 14 00:00:00 EST 2019

Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks.

Cell reports

Stein BD,Calzolari D,Hellberg K,Hu YS,He L,Hung CM,Toyama EQ,Ross DS,Lillemeier BF,Cantley LC,Yates JR,Shaw RJ

Tue Dec 03 00:00:00 EST 2019

HIF1α Suppresses Tumor Cell Proliferation through Inhibition of Aspartate Biosynthesis.

Cell reports

Meléndez-Rodríguez F,Urrutia AA,Lorendeau D,Rinaldi G,Roche O,Böğürcü-Seidel N,Ortega Muelas M,Mesa-Ciller C,Turiel G,Bouthelier A,Hernansanz-Agustín P,Elorza A,Escasany E,Li QOY,Torres-Capelli M,Tello D,Fuertes E,Fraga E,Martínez-Ruiz A,Pérez B,Giménez-Bachs JM,Salinas-Sánchez AS,Acker T,Sánchez Prieto R,Fendt SM,De Bock K,Aragonés J

Tue Feb 26 00:00:00 EST 2019

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

American journal of respiratory cell and molecular biology

Hamanaka RB,O'Leary EM,Witt LJ,Tian Y,Gökalp GA,Meliton AY,Dulin NO,Mutlu GM

Fri Nov 01 00:00:00 EDT 2019

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cell research

Cai WF,Zhang C,Wu YQ,Zhuang G,Ye Z,Zhang CS,Lin SC

Wed Aug 01 00:00:00 EDT 2018

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cell research

Cai WF,Zhang C,Wu YQ,Zhuang G,Ye Z,Zhang CS,Lin SC

Wed Aug 01 00:00:00 EDT 2018

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Nature communications

Wang YQ,Wang HL,Xu J,Tan J,Fu LN,Wang JL,Zou TH,Sun DF,Gao QY,Chen YX,Fang JY

Wed Feb 07 00:00:00 EST 2018

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Nature communications

Wang YQ,Wang HL,Xu J,Tan J,Fu LN,Wang JL,Zou TH,Sun DF,Gao QY,Chen YX,Fang JY

Wed Feb 07 00:00:00 EST 2018

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Nature communications

Feist M,Schwarzfischer P,Heinrich P,Sun X,Kemper J,von Bonin F,Perez-Rubio P,Taruttis F,Rehberg T,Dettmer K,Gronwald W,Reinders J,Engelmann JC,Dudek J,Klapper W,Trümper L,Spang R,Oefner PJ,Kube D

Tue Apr 17 00:00:00 EDT 2018

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Nature communications

Feist M,Schwarzfischer P,Heinrich P,Sun X,Kemper J,von Bonin F,Perez-Rubio P,Taruttis F,Rehberg T,Dettmer K,Gronwald W,Reinders J,Engelmann JC,Dudek J,Klapper W,Trümper L,Spang R,Oefner PJ,Kube D

Tue Apr 17 00:00:00 EDT 2018

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Cell metabolism

Chen Q,Kirk K,Shurubor YI,Zhao D,Arreguin AJ,Shahi I,Valsecchi F,Primiano G,Calder EL,Carelli V,Denton TT,Beal MF,Gross SS,Manfredi G,D'Aurelio M

Tue May 01 00:00:00 EDT 2018

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Molecular medicine reports

Liu M,Liu S,Tan W,Tang F,Long J,Li Z,Liang B,Chu C,Yang J

Fri Dec 01 00:00:00 EST 2017

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Molecular medicine reports

Liu M,Liu S,Tan W,Tang F,Long J,Li Z,Liang B,Chu C,Yang J

Fri Dec 01 00:00:00 EST 2017

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

Enhanced synthesis and diminished degradation of hydrogen sulfide in experimental colitis: a site-specific, pro-resolution mechanism.

PloS one

Flannigan KL,Ferraz JG,Wang R,Wallace JL

Mon Apr 14 00:00:00 EDT 2014

Enhanced synthesis and diminished degradation of hydrogen sulfide in experimental colitis: a site-specific, pro-resolution mechanism.

PloS one

Flannigan KL,Ferraz JG,Wang R,Wallace JL

Mon Apr 14 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: aspartate aminotransferase 1; Aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; Glutamate oxaloacetate transaminase 1; glutamate oxaloacetate transaminase 1, soluble; glutamic-oxaloacetic transaminase 1, soluble; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; Transaminase A

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Gene Aliases: AI789014; Aspat; AST1; ASTQTL1; cAspAT; cCAT; Gaspat; GIG18; Got-1; GOT1

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UniProt ID: (Human) P17174, (Rat) P13221, (Mouse) P05201

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Entrez Gene ID: (Human) 2805, (Rat) 24401, (Mouse) 14718

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Function(s)

L-aspartate:2-oxoglutarate aminotransferase activity phosphatidylserine decarboxylase activity pyridoxal phosphate binding carboxylic acid binding identical protein binding L-cysteine:2-oxoglutarate aminotransferase activity L-phenylalanine:2-oxoglutarate aminotransferase activity catalytic activity transaminase activity transferase activity transaminase

Process(es)

gluconeogenesis 2-oxoglutarate metabolic process oxaloacetate metabolic process glycerol biosynthetic process aspartate metabolic process aspartate biosynthetic process aspartate catabolic process glutamate metabolic process Notch signaling pathway cellular amino acid biosynthetic process glutamate catabolic process to aspartate glutamate catabolic process to 2-oxoglutarate cellular response to insulin stimulus response to glucocorticoid fatty acid homeostasis dicarboxylic acid metabolic process cellular amino acid metabolic process biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GOT1 Polyclonal Antibody

GOT1 Antibody (14886-1-AP) in ICC/IF

Product Details

14886-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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