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GOT1 Antibody (PA5-97800) in ICC/IF
Immunofluorescent analysis of GOT1 in HepG2 cells using a GOT1 polyclonal antibody (Product # PA5-97800) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Product Details
PA5-97800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Aspartate aminotransferase, cytoplasmic protein (2-411AA)
Conjugate
Form
Liquid
Concentration
5 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2812415
Target Information
GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aspartate aminotransferase 1; Aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; Glutamate oxaloacetate transaminase 1; glutamate oxaloacetate transaminase 1, soluble; glutamic-oxaloacetic transaminase 1, soluble; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; Transaminase A
Gene Aliases: AI789014; AST1; ASTQTL1; cAspAT; cCAT; GIG18; Got-1; GOT1
UniProt ID: (Human) P17174, (Mouse) P05201
Entrez Gene ID: (Human) 2805, (Mouse) 14718
L-aspartate:2-oxoglutarate aminotransferase activity
phosphatidylserine decarboxylase activity
pyridoxal phosphate binding
carboxylic acid binding
identical protein binding
L-cysteine:2-oxoglutarate aminotransferase activity
L-phenylalanine:2-oxoglutarate aminotransferase activity
catalytic activity
transaminase activity
transferase activity
transaminase
gluconeogenesis
2-oxoglutarate metabolic process
oxaloacetate metabolic process
glycerol biosynthetic process
aspartate metabolic process
aspartate biosynthetic process
aspartate catabolic process
glutamate metabolic process
Notch signaling pathway
cellular amino acid biosynthetic process
glutamate catabolic process to aspartate
glutamate catabolic process to 2-oxoglutarate
cellular response to insulin stimulus
response to glucocorticoid
fatty acid homeostasis
cellular amino acid metabolic process
biosynthetic process
dicarboxylic acid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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