Proteintech

GOT2 Polyclonal Antibody

GOT2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

GOT2 Antibody (14800-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using GOT2 antibody 14800-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GOT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

14800-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GOT2 Fusion Protein Ag6514 (82-430 aa encoded by BC000525)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KAEAQIAAK NLDKEYLPIG GLAEFCKASA ELALGENSEV LKSGRFVTVQ TISGTGALRI GASFLQRFFK FSRDVFLPKP TWGNHTPIFR DAGMQLQGYR YYDPKTCGFD FTGAVEDISK IPEQSVLLLH ACAHNPTGVD PRPEQWKEIA TVVKKRNLFA FFDMAYQGFA SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTMVCKDADE AKRVESQLKI LIRPMYSNPP LNGARIAAAI LNTPDLRKQW LQEVKVMADR IIGMRTQLVS NLKKEGSTHN WQHITDQIGM FCFTGLKPEQ VERLIKEFSI YMTKDGRISV AGVTSSNVGY LAHAIHQATK (82-430 aa encoded by BC000525)

Target Information

GOT2 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation.

Frontiers in microbiology

Li F,Fu X,Luo X,Lin Q,Liang H,Niu Y,Liu L,Li N

Tue Apr 18 00:00:00 EDT 2023

ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells.

Cancer research

Hu Q,Dai J,Zhang Z,Yu H,Zhang J,Zhu X,Qin Y,Zhang L,Zhang P

Mon May 15 00:00:00 EDT 2023

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

EMBO molecular medicine

Southwell N,Primiano G,Nadkarni V,Attarwala N,Beattie E,Miller D,Alam S,Liparulo I,Shurubor YI,Valentino ML,Carelli V,Servidei S,Gross SS,Manfredi G,Chen Q,D'Aurelio M

Mon Jul 10 00:00:00 EDT 2023

Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells.

eLife

Hart ML,Quon E,Vigil ABG,Engstrom IA,Newsom OJ,Davidsen K,Hoellerbauer P,Carlisle SM,Sullivan LB

Wed Mar 08 00:00:00 EST 2023

Gaseous signal molecule SO₂ regulates autophagy through PI3K/AKT pathway inhibits cardiomyocyte apoptosis and improves myocardial fibrosis in rats with type II diabetes.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Zhao J,Wu Q,Yang T,Nie L,Liu S,Zhou J,Chen J,Jiang Z,Xiao T,Yang J,Chu C

Tue Nov 01 00:00:00 EDT 2022

A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H₂S oxidation.

The Journal of biological chemistry

Kumar R,Landry AP,Guha A,Vitvitsky V,Lee HJ,Seike K,Reddy P,Lyssiotis CA,Banerjee R

Sat Jan 01 00:00:00 EST 2022

NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression.

Frontiers in cell and developmental biology

Tsujimoto G,Ito R,Yoshikawa K,Ueki C,Okada N

Tue Sep 13 00:00:00 EDT 2022

Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3^SPOP E3 ligase in cancer cells.

Nature communications

Zhou Q,Lin W,Wang C,Sun F,Ju S,Chen Q,Wang Y,Chen Y,Li H,Wang L,Hu Z,Jin H,Wang X,Sun Y

Tue May 31 00:00:00 EDT 2022

Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.

Frontiers in cell and developmental biology

Zhao Y,Zhang J,Wang S,Jiang Q,Xu K

Sat Sep 25 00:00:00 EDT 2021

The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis.

The Journal of biological chemistry

Vitvitsky V,Kumar R,Libiad M,Maebius A,Landry AP,Banerjee R

Tue Aug 31 00:00:00 EDT 2021

Hepatic mTORC1 signaling activates ATF4 as part of its metabolic response to feeding and insulin.

Molecular metabolism

Byles V,Cormerais Y,Kalafut K,Barrera V,Hughes Hallett JE,Sui SH,Asara JM,Adams CM,Hoxhaj G,Ben-Sahra I,Manning BD

Mon Nov 01 00:00:00 EDT 2021

Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.

Cell stem cell

Qi L,Martin-Sandoval MS,Merchant S,Gu W,Eckhardt M,Mathews TP,Zhao Z,Agathocleous M,Morrison SJ

Thu Nov 04 00:00:00 EDT 2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Theranostics

Zhang X,Yu K,Ma L,Qian Z,Tian X,Miao Y,Niu Y,Xu X,Guo S,Yang Y,Wang Z,Xue X,Gu C,Fang W,Sun J,Yu Y,Wang J

Thu Jul 29 00:00:00 EDT 2021

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Cell metabolism

Wang YP,Sharda A,Xu SN,van Gastel N,Man CH,Choi U,Leong WZ,Li X,Scadden DT

Tue May 04 00:00:00 EDT 2021

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Cell metabolism

Wang YP,Sharda A,Xu SN,van Gastel N,Man CH,Choi U,Leong WZ,Li X,Scadden DT

Tue May 04 00:00:00 EDT 2021

USP29 coordinates MYC and HIF1α stabilization to promote tumor metabolism and progression.

Oncogene

Tu R,Kang W,Yang M,Wang L,Bao Q,Chen Z,Dong Y,Wang J,Jiang J,Liu H,Qing G

Mon Nov 01 00:00:00 EDT 2021

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

The Journal of experimental medicine

Liu M,Wang Y,Yang C,Ruan Y,Bai C,Chu Q,Cui Y,Chen C,Ying G,Li B

Mon Mar 02 00:00:00 EST 2020

Ischemic Neuroprotectant PKCε Restores Mitochondrial Glutamate Oxaloacetate Transaminase in the Neuronal NADH Shuttle after Ischemic Injury.

Translational stroke research

Xu J,Khoury N,Jackson CW,Escobar I,Stegelmann SD,Dave KR,Perez-Pinzon MA

Mon Jun 01 00:00:00 EDT 2020

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

American journal of respiratory cell and molecular biology

Hamanaka RB,O'Leary EM,Witt LJ,Tian Y,Gökalp GA,Meliton AY,Dulin NO,Mutlu GM

Fri Nov 01 00:00:00 EDT 2019

Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation.

Molecular oncology

Hong R,Zhang W,Xia X,Zhang K,Wang Y,Wu M,Fan J,Li J,Xia W,Xu F,Chen J,Wang S,Zhan Q

Mon Apr 01 00:00:00 EDT 2019

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cell research

Cai WF,Zhang C,Wu YQ,Zhuang G,Ye Z,Zhang CS,Lin SC

Wed Aug 01 00:00:00 EDT 2018

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cell research

Cai WF,Zhang C,Wu YQ,Zhuang G,Ye Z,Zhang CS,Lin SC

Wed Aug 01 00:00:00 EDT 2018

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Nature communications

Wang YQ,Wang HL,Xu J,Tan J,Fu LN,Wang JL,Zou TH,Sun DF,Gao QY,Chen YX,Fang JY

Wed Feb 07 00:00:00 EST 2018

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Nature communications

Wang YQ,Wang HL,Xu J,Tan J,Fu LN,Wang JL,Zou TH,Sun DF,Gao QY,Chen YX,Fang JY

Wed Feb 07 00:00:00 EST 2018

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Nature communications

Feist M,Schwarzfischer P,Heinrich P,Sun X,Kemper J,von Bonin F,Perez-Rubio P,Taruttis F,Rehberg T,Dettmer K,Gronwald W,Reinders J,Engelmann JC,Dudek J,Klapper W,Trümper L,Spang R,Oefner PJ,Kube D

Tue Apr 17 00:00:00 EDT 2018

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Nature communications

Feist M,Schwarzfischer P,Heinrich P,Sun X,Kemper J,von Bonin F,Perez-Rubio P,Taruttis F,Rehberg T,Dettmer K,Gronwald W,Reinders J,Engelmann JC,Dudek J,Klapper W,Trümper L,Spang R,Oefner PJ,Kube D

Tue Apr 17 00:00:00 EDT 2018

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Nature communications

Feist M,Schwarzfischer P,Heinrich P,Sun X,Kemper J,von Bonin F,Perez-Rubio P,Taruttis F,Rehberg T,Dettmer K,Gronwald W,Reinders J,Engelmann JC,Dudek J,Klapper W,Trümper L,Spang R,Oefner PJ,Kube D

Tue Apr 17 00:00:00 EDT 2018

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Nature communications

Feist M,Schwarzfischer P,Heinrich P,Sun X,Kemper J,von Bonin F,Perez-Rubio P,Taruttis F,Rehberg T,Dettmer K,Gronwald W,Reinders J,Engelmann JC,Dudek J,Klapper W,Trümper L,Spang R,Oefner PJ,Kube D

Tue Apr 17 00:00:00 EDT 2018

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Cell metabolism

Chen Q,Kirk K,Shurubor YI,Zhao D,Arreguin AJ,Shahi I,Valsecchi F,Primiano G,Calder EL,Carelli V,Denton TT,Beal MF,Gross SS,Manfredi G,D'Aurelio M

Tue May 01 00:00:00 EDT 2018

HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.

Journal of cellular and molecular medicine

Li W,Chen C,Zhao X,Ye H,Zhao Y,Fu Z,Pan W,Zheng S,Wei L,Nong T,Li Z,Chen R

Wed Nov 01 00:00:00 EDT 2017

HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.

Journal of cellular and molecular medicine

Li W,Chen C,Zhao X,Ye H,Zhao Y,Fu Z,Pan W,Zheng S,Wei L,Nong T,Li Z,Chen R

Wed Nov 01 00:00:00 EDT 2017

Glutamine metabolism regulates autophagy-dependent mTORC1 reactivation during amino acid starvation.

Nature communications

Tan HWS,Sim AYL,Long YC

Thu Aug 24 00:00:00 EDT 2017

Glutamine metabolism regulates autophagy-dependent mTORC1 reactivation during amino acid starvation.

Nature communications

Tan HWS,Sim AYL,Long YC

Thu Aug 24 00:00:00 EDT 2017

Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors.

Oncotarget

Kitazawa S,Ebara S,Ando A,Baba Y,Satomi Y,Soga T,Hara T

Tue Apr 25 00:00:00 EDT 2017

Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors.

Oncotarget

Kitazawa S,Ebara S,Ando A,Baba Y,Satomi Y,Soga T,Hara T

Tue Apr 25 00:00:00 EDT 2017

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: aspartate aminotransferase 2; Aspartate aminotransferase, mitochondrial; aspartate transaminase 2; FABP-1; FABPpm; Fatty acid-binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; glutamate oxaloacetate transaminase 2, mitochondrial; Glutamate oxaloacetate transaminase 2, mitochondrial (aspartate aminotransferase 2); glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2); Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; mAspAT; mitochondrial aspartate aminotransferase; plasma membrane fatty acid binding protein; Plasma membrane-associated fatty acid-binding protein; Transaminase A

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Gene Aliases: AL022787; ASPATA; FABP-pm; Got-2; GOT2; KAT4; KATIV; KYAT4; Maat; mAspAT; mitAAT

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UniProt ID: (Human) P00505, (Mouse) P05202, (Rat) P00507

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Entrez Gene ID: (Human) 2806, (Mouse) 14719, (Rat) 25721

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Function(s)

L-aspartate:2-oxoglutarate aminotransferase activity phospholipid binding kynurenine-oxoglutarate transaminase activity amino acid binding enzyme binding pyridoxal phosphate binding identical protein binding protein homodimerization activity poly(A) RNA binding L-phenylalanine:2-oxoglutarate aminotransferase activity catalytic activity transaminase activity transferase activity carboxylic acid binding transaminase

Process(es)

gluconeogenesis 2-oxoglutarate metabolic process oxaloacetate metabolic process aspartate metabolic process aspartate biosynthetic process aspartate catabolic process glutamate metabolic process cellular amino acid biosynthetic process fatty acid transport 4-hydroxyproline catabolic process glutamate catabolic process to aspartate glutamate catabolic process to 2-oxoglutarate response to ethanol L-kynurenine metabolic process cellular amino acid metabolic process transport lipid transport biosynthetic process dicarboxylic acid metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GOT2 Polyclonal Antibody

GOT2 Antibody (14800-1-AP) in ICC/IF

Product Details

14800-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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