Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
GPCR/G-protein complex-stabilizing scFv Recombinant Mouse Monoclonal Antibody (scFv16)
FIGURE: 1 / 2
GPCR/G-protein complex-stabilizing scFv Antibody (703976-1MG)
A tagged GPCR was used to pull down a GPCR-heterotrimeric G protein complex that is stabilized by scFv16 (Product # 703976). A) Size exclusion chromatography (SEC) chromatogram of the purified complex. B) Highlighted sample from SEC was run on SDS-PAGE gel and Coomassie stained. All components of the active complex are present. C) Direct visualization of GPCR-G protein complex by negative stain TEM showing features characteristic for active GPCR complexes that are intact. Data courtesy Dr. David M. Thal, Monash Institute of Pharmaceutical ... View More
Product Details
703976-1MG
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
scFv16
Immunogen
Rhodopsin/Gi1
Conjugate
Form
Liquid
Concentration
2.0 mg/mL
Purification
Affinity chromatography
Storage buffer
25mM HEPES, pH 7.5, with 10% glycerol
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2896321
Product Specific Information
The scFv antibody stabilizes GPCR/G-protein complexes for cryo-EM of active structures. It recognizes an interface between G alpha and G beta/gamma subunits, and confers resistance to nucleotide exchange-triggered dissociation of the heterotrimeric G-protein. In addition to members of the Gi/o family, this antibody fragment can also be applied to chimeric variants and stabilize other G-protein subtypes including Gs complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support