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Invitrogen
GPNMB Monoclonal Antibody (CTSREVL), eFluor™ 660, eBioscience™
FIGURE: 1 / 2
GPNMB Antibody (50-5708-82) in Flow
Staining of mouse thioglycolate-elicited peritoneal exudate cells with Anti-Mouse F4/80 Antigen eFluor® 450 (Product # 48-4801-82) and 0.5 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.5 µg of Anti-Mouse GPNMB eFluor® 660 (right). Total cells were used for analysis.
Product Details
50-5708-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CTSREVL
Immunogen
FC-tagged full protein expressed in insect cells
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574239
Product Specific Information
Description: This CTSREVL monoclonal antibody recognizes human GPNMB also known as Osteoactivin, DC-HIL and HGFIN. This type-1 transmembrane glycoprotein was described independently as a bone development factor, tumor related molecule, neurokinin, and immune regulator. Expressed on macrophages and dendritic cells, GPNMB is a ligand for proteoglycan Syndecan-4 (SD-4), which is found on T cells and is known to inhibit their activation and proliferation. Hence, surface expression of GPNMB by myeloid cells may be indicative of their tolerogenic properties. Various cancers express GPNMB, and it is suspected to help in immune evasion. Treatment with IL-10 and various immunosuppressive drugs (e.g., dexamethasone) upregulate GPNMB on macrophages and dendritic cells. GPNMB may also be secreted.
The CTSREVL antibody will work on paraformaldehyde-fixed cells.
Applications Reported: This CTSREVL antibody has been reported for use in flow cytometric analysis.
Applications Tested: This CTSREVL antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; Dendritic cell-associated transmembrane protein; iris pigment dispersion; Osteoactivin; Transmembrane glycoprotein NMB
Gene Aliases: DC-HIL; Dchil; Gpnmb; Hgfin; ipd; Nmb
UniProt ID: (Mouse) Q99P91
Entrez Gene ID: (Mouse) 93695
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