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GPNMB Antibody (17-9838-42) in Flow
Normal human blood monocyte-derived macrophages were cultured in the presence of Human M-CSF Recombinant Protein (Product # 14-8789-80) and Human IL-10 Recombinant Protein (Product # 14-8109-80), and then stained with CD11b Monoclonal Antibody, FITC (Product # 11-0118-42) and Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or GPNMB Monoclonal Antibody, APC (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 506 (Product # 65-0866-18).
Product Details
17-9838-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HOST5DS
Immunogen
FC-tagged full protein expressed in insect cells
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802235
Product Specific Information
Description: This HOST5DS monoclonal antibody recognizes human GPNMB also known as Osteoactivin, DC-HIL and HGFIN. This type-1 transmembrane glycoprotein was described independently as a bone development factor, tumor related molecule, neurokinin, and immune regulator. Expressed on macrophages and dendritic cells, GPNMB is a ligand for proteoglycan Syndecan-4 (SD-4), which is found on T cells, and is known to inhibit their activation and proliferation. Hence, surface expression of GPNMB by myeloid cells may be indicative of their tolerogenic properties. Various cancers express GPNMB, and it is suspected to help in immune evasion. Treatment with IL-10 and various immunosuppressive drugs (e.g., dexamethasone) upregulate GPNMB on macrophages and dendritic cells. THP-1 and U937 cell lines constitutively express GPNMB. GPNMB may also be secreted.
The HOST5DS antibody will work on paraformaldehyde-fixed and permeabilized cells, and can be used for intracellular staining. Please, note that enzymatic tissue dissociation may reduce the amount of surface GPNMB, and thereby negatively impact the brightness of the staining.
Applications Reported: This HOST5DS antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HOST5DS antibody has been pre-diluted and tested by flow cytometric analysis of normal human blood monocyte-derived macrophages. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glycoprotein (transmembrane) nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; hematopoietic growth factor inducible neurokinin-1; Hematopoietic growth factor inducible neurokinin-1 type; osteoactivin; transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB
Gene Aliases: GPNMB; HGFIN; NMB; UNQ1725/PRO9925
UniProt ID: (Human) Q14956
Entrez Gene ID: (Human) 10457
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