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Invitrogen
GPNMB Recombinant Rabbit Monoclonal Antibody (PSH0-82)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 13
GPNMB Antibody (MA5-49360) in ICC/IF
Immunofluorescence analysis of GPNMB using L929 cells. The cells were fixed 4% paraformaldehyde for 10 minutes, permeabilized with 0.05% Triton™ X-100 in PBS for 20 minutes, and blocked with 2% negative goat serum for 30 minutes at room temperature. The cells were labeled with GPNMB Rabbit Recombinant Monoclonal Antibody (PSH0-82) (Product # MA5-49360) at 1:100 dilution in 2% negative goat serum overnight at 4°C and then with iFluor™ 488 Goat Anti-Rabbit IgG H&L secondary antibody (1:1,000 dilution) for 1 hour at room temperature (green). ... View More
Product Details
MA5-49360
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-82
Immunogen
Recombinant protein within human gpnmb aa 1-498 / 572.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3075017
Product Specific Information
Sequence Similarities: 98% Mouse/Rat.
Tissue Specificity: Widely expressed, but very low expression, if any, in the brain (PubMed:12609765, PubMed:16609006). Expressed in the epidermis with higher levels in melanocytes compared with keratinocytes and Langerhans cells (at protein level) (PubMed:29336782). Expressed in peripheral blood, but not bone marrow mononuclear cells (PubMed:12609765). Expressed in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level) (PubMed:16489096). May be overexpressed in various cancers, including melanoma and glioblastoma multiforme (PubMed:7814155, PubMed:16489096, PubMed:16609006).
Positive Control: L929 cell lysate, A375 cell lysate, U-937 cell lysate, rat heart tissue lysate, B16F1 cell lysates, human lung tissue, L929.
Subcellular Location: Cell membrane, Melanosome membrane, Early endosome membrane.
Predicted band size: 64 kDa.
Target Information
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; Dendritic cell-associated transmembrane protein; glycoprotein (transmembrane) nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; hematopoietic growth factor inducible neurokinin-1; Hematopoietic growth factor inducible neurokinin-1 type; iris pigment dispersion; Osteoactivin; transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB
Gene Aliases: DC-HIL; Dchil; GPNMB; HGFIN; ipd; NMB; UNQ1725/PRO9925
UniProt ID: (Human) Q14956, (Mouse) Q99P91
Entrez Gene ID: (Human) 10457, (Rat) 113955, (Mouse) 93695
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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