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GPR109A/HCAR2 (extracellular) Antibody (AHR-012-200UL) in Flow
Cell surface detection of GPR109A by indirect flow cytometry in live intact mouse J774 macrophage cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti- GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (2.5μg) + goat- Anti-rabbit-FITC.
Product Details
AHR-012-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)DNYVHNWDWRFGG, corresponding to amino acid residues 82-94 of mouse HCAR2, Extracellular, 1st loop.
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
GPR109A (also known as HCA2, HM74a, and NIACR1) is a 42kDa G-protein coupled receptor that belongs to a family of three hydroxyl-carboxylic acid receptors, which also include GPR109B and GPR81. GPR109A has two known ligands: niacin and butyrate (or its derivative 3-hydroxybutyrate). The endogenous ligands for GPR109B and GPR81 are 3-hydroxyl-octanoic acid and 2-hydroxy-propanoate (lactate), respectively. GPR109A is expressed on adipocytes, neutrophils, Langerhans cells, keratinocytes, intestinal epithelial cells, and intestinal colonic macrophages. Niacin, also known as nicotinic acid or vitamin B3, is known to improve cholesterol metabolism (LDL/HDL ratio) and reduce inflammation. It has been demonstrated that this effect is mediated through GPR109A. Notably, GPR109A also has an essential role in suppressing inflammation and cancer in the colon. Both GPR109A ligands are produced by colonic commensal bacteria. In mice, GPR109A expression on intestinal epithelial cells and DCs/macrophages contributes to the suppression of colitis and colon cancer. In humans, GPR109A is silenced in colon carcinoma cells by DNA methylation, which increases inflammation in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein-coupled receptor 109A; G protein-coupled receptor 109B; G protein-coupled receptor HM74a; G-protein coupled receptor 109; G-protein coupled receptor 109A; G-protein coupled receptor HM74; G-protein coupled receptor HM74A; hydroxy-carboxylic acid receptor 2; Hydroxycarboxylic acid receptor 2; interferon-gamma inducible gene, Puma-g; Niacin receptor 1; Nicotinic acid receptor; Protein PUMA-G; PUMA-G. GPR109; putative seven transmembrane spanning receptor
Gene Aliases: Gpr109; GPR109A; Gpr109b; HCA2; HCAR2; HM74; HM74A; HM74b; mHM74b; NIACR1; PUMA-G; PUMAG
UniProt ID: (Human) Q8TDS4, (Mouse) Q9EP66
Entrez Gene ID: (Human) 338442, (Mouse) 80885
neutrophil apoptotic process
G-protein coupled receptor signaling pathway
positive regulation of neutrophil apoptotic process
negative regulation of lipid catabolic process
positive regulation of adiponectin secretion
apoptotic process
signal transduction
purinergic nucleotide receptor signaling pathway
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