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GPR143 Antibody (PA5-120094) in WB
Western blot analysis of GPR143 in extracts of various cell lines. Samples were incubated with GPR143 Polyclonal antibody (Product # PA5-120094) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-120094
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 314-404 of human GPR143 (NP_000264.2).
Conjugate
Form
Liquid
Concentration
0.98 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913666
Product Specific Information
Positive test controls include: U-251MG, BT-474, SW480, Mouse liver, Mouse kidney, Rat kidney. The target is usually found in the following locations: Apical cell membrane, Golgi apparatus, Lysosome membrane, Melanosome membrane, Multi-pass membrane protein.
Immunogen sequence: TGCSLGFQSP RKEIQWESLT TSAAEGAHPS PLMPHENPAS GKVSQVGGQT SDEALSMLSE GSDASTIEIH TASESCNKNE GDPALPTHGD L
Target Information
Ocular albinism type 1 protein is a conserved integral membrane protein with seven transmembrane domains. It is expressed in the eye and epidermal melanocytes. Defects in GPR143 are the cause of ocular albinism type 1 (OA1); also known as Nettleship-Falls type ocular albinism. OA1 is an X-linked disorder characterized by severe impairment of visual acuity, retinal hypopigmentation and the presence of macromelanosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G-protein coupled receptor 143; homolog of human ocular albinism 1 (Nettleship-Falls); MOA1; ocular albinism 1; Ocular albinism type 1 protein; Ocular albinism type 1 protein homolog
Gene Aliases: GPR143; NYS6; OA1; RGD1565799
UniProt ID: (Human) P51810, (Mouse) P70259
Entrez Gene ID: (Human) 4935, (Mouse) 18241, (Rat) 302619
eye pigment biosynthetic process
signal transduction
G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
visual perception
melanosome localization
melanosome transport
melanosome organization
calcium-mediated signaling using intracellular calcium source
phosphatidylinositol-mediated signaling
regulation of calcium-mediated signaling
regulation of melanosome transport
regulation of melanosome organization
chemical synaptic transmission
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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