Proteintech

GPT Polyclonal Antibody

FIGURE: 1 / 7

GPT Antibody (16897-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 16897-1-AP) GPT antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

GPT Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16897-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GPT Fusion Protein Ag10424 (146-496 aa encoded by BC018207)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GIPADPNNV FLSTGASDAI VTVLKLLVAG EGHTRTGVLI PIPQYPLYSA TLAELGAVQV DYYLDEERAW ALDVAELHRA LGQARDHCRP RALCVINPGN PTGQVQTREC IEAVIRFAFE ERLFLLADEV YQDNVYAAGS QFHSFKKVLM EMGPPYAGQQ ELASFHSTSK GYMGECGFRG GYVEVVNMDA AVQQQMLKLM SVRLCPPVPG QALLDLVVSP PAPTDPSFAQ FQAEKQAVLA ELAAKAKLTE QVFNEAPGIS CNPVQGAMYS FPRVQLPPRA VERAQELGLA PDMFFCLRLL EETGICVVPG SGFGQREGTY HFRMTILPPL EKLRLLLEKL SRFHAKFTLE YS (146-496 aa encoded by BC018207)

Target Information

GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

EMBO molecular medicine

Southwell N,Primiano G,Nadkarni V,Attarwala N,Beattie E,Miller D,Alam S,Liparulo I,Shurubor YI,Valentino ML,Carelli V,Servidei S,Gross SS,Manfredi G,Chen Q,D'Aurelio M

Mon Jul 10 00:00:00 EDT 2023

Glutamine synthetase limits β-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1.

The Journal of clinical investigation

Dai W,Shen J,Yan J,Bott AJ,Maimouni S,Daguplo HQ,Wang Y,Khayati K,Guo JY,Zhang L,Wang Y,Valvezan A,Ding WX,Chen X,Su X,Gao S,Zong WX

Thu Dec 15 00:00:00 EST 2022

Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.

Cancer medicine

Cai X,Qu L,Yang J,Xu J,Sun L,Wei X,Qu X,Bai T,Guo Z,Zhu Y

Sat Aug 01 00:00:00 EDT 2020

Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.

Mediators of inflammation

Wang S,Sun Y,Bai Y,Zhou N,Chen N,Miller EJ,Zhang Y,Li W

Wed Nov 03 00:00:00 EDT 2021

T Cell Activation Depends on Extracellular Alanine.

Cell reports

Ron-Harel N,Ghergurovich JM,Notarangelo G,LaFleur MW,Tsubosaka Y,Sharpe AH,Rabinowitz JD,Haigis MC

Tue Sep 17 00:00:00 EDT 2019

Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.

Oncotarget

Bharti SK,Mironchik Y,Wildes F,Penet MF,Goggins E,Krishnamachary B,Bhujwalla ZM

Fri Mar 16 00:00:00 EDT 2018

Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.

Oncotarget

Bharti SK,Mironchik Y,Wildes F,Penet MF,Goggins E,Krishnamachary B,Bhujwalla ZM

Fri Mar 16 00:00:00 EDT 2018

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Cell metabolism

Chen Q,Kirk K,Shurubor YI,Zhao D,Arreguin AJ,Shahi I,Valsecchi F,Primiano G,Calder EL,Carelli V,Denton TT,Beal MF,Gross SS,Manfredi G,D'Aurelio M

Tue May 01 00:00:00 EDT 2018

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: alanine aminotransferase; Alanine aminotransferase 1; ALT1; Glutamate pyruvate transaminase 1; glutamic pyruvate transaminase; glutamic pyruvic transaminase (alanine aminotrasferase); glutamic pyruvic transaminase 1, soluble; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; glutamic-alanine transaminase 1; glutamic-pyruvate transaminase (alanine aminotransferase); GPT 1

View more View less

Gene Aliases: 1300007J06Rik; 2310022B03Rik; AAT1; ALT; ALT1; GPT; Gpt-1; GPT1

View more View less

UniProt ID: (Human) P24298, (Rat) P25409, (Mouse) Q8QZR5

View more View less

Entrez Gene ID: (Human) 2875, (Rat) 81670, (Mouse) 76282

View more View less

Function(s)

L-alanine:2-oxoglutarate aminotransferase activity pyridoxal phosphate binding catalytic activity transaminase activity transferase activity transaminase

Process(es)

cellular amino acid biosynthetic process L-alanine catabolic process biological_process biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

GPT Polyclonal Antibody

GPT Antibody (16897-1-AP) in WB

Product Details

16897-1-AP

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!