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Invitrogen
GRASP65 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
GRASP65 Antibody (PA3-910) in ICC/IF
Immunofluorescent analysis of Grasp65 (green) in HeLa cells either left untreated or treated 5 µg/mL Brefeldin A for 4 hours. The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Grasp65 rabbit polyclonal antibody (Product # PA3-910) at a dilution of 1:200 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibo... View More
Product Details
PA3-910
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
GST fusion protein containing C-terminal residues (202-447) of rat Grasp65.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2113207
Product Specific Information
PA3-910 detects Grasp65 from human, mouse, and rat samples.
PA3-910 has been successfully used in Western blot, IHC (paraffin), immunofluorescence, immunocytochemistry and immunoprecipitation procedures.
The PA3-910 immunogen is a GST fustion protein containing the C-terminal residues (202-447) of rat Grasp65.
Target Information
Grasp65 is a structural protein of the golgi apparatus. It has been implicated in the stacking of golgi cisternae. Grasp65 is shown to have the properties to bind surfaces together in a mitotically regulated matter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly and stacking protein 1; golgi reassembly stacking protein 1, 65kDa; Golgi reassembly-stacking protein 1; Golgi reassembly-stacking protein of 65 kDa; GOLPH5; GRASP65
Gene Aliases: 5430411C10Rik; GOLPH5; GORASP1; GRASP65; P65
UniProt ID: (Human) Q9BQQ3, (Mouse) Q91X51
Entrez Gene ID: (Human) 64689, (Rat) 56082, (Mouse) 74498
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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