Invitrogen

GRP78 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

24 Published Figures

40 References

View all (63) GRP78 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GRP78 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 33

GRP78 Antibody (PA1-014A) in ICC/IF

Immunofluorescence analysis of GRP78 was performed using 70% confluent log phase MCF7 cells and MCF7 cells treated with 1 µM of Thapsigargin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GRP78 Rabbit Polyclonal Antibody (Product # PA1-014A) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secon... View More

GRP78 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

GRP78 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

GRP78 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using GRP78 Polyclonal Antibody (Product # PA1-014A), shows increased expression of proteins in MCF-7 and HeLa upon treatment with Thapsigargin. {TM}

Product Details

PA1-014A

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 31 publications 31 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 4 publications 4 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP78.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_559362

Product Specific Information

PA1-014A detects glucose regulated protein/BiP (GRP78) human, rat, mouse, and canine samples.

PA1-014A has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 78 kDa protein representing GRP78 from rat liver lysates.

The PA1-014A immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP78.

Reconstitute with PBS.

Target Information

GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78’s involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Morphological and Functional Effects of Ultrasound on Blood-Brain Barrier Transitory Opening: An In Vitro Study on Rat Brain Endothelial Cells.

Cells

Branca JJV,Boninsegna M,Morucci G,Carrino D,Nicoletti C,Paternostro F,Gulisano M,Bocchi L,Pacini A

PA1-014A was used in Western Blotting to carry out an in depth in vitro molecular and morphological study on the effects of US treatment on the BBB.

Tue Jan 03 00:00:00 EST 2023

Morphological and Functional Effects of Ultrasound on Blood-Brain Barrier Transitory Opening: An In Vitro Study on Rat Brain Endothelial Cells.

Cells

Branca JJV,Boninsegna M,Morucci G,Carrino D,Nicoletti C,Paternostro F,Gulisano M,Bocchi L,Pacini A

PA1-014A was used in Western Blotting to carry out an in depth in vitro molecular and morphological study on the effects of US treatment on the BBB.

Tue Jan 03 00:00:00 EST 2023

Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia.

Journal of extracellular vesicles

Korvenlaita N,Gómez-Budia M,Scoyni F,Pistono C,Giudice L,Eamen S,Loppi S,de Sande AH,Huremagic B,Bouvy-Liivrand M,Heinäniemi M,Kaikkonen MU,Cheng L,Hill AF,Kanninen KM,Jenster GW,van Royen ME,Ramiro L,Montaner J,Batkova T,Mikulik R,Giugno R,Jolkkonen J,Korhonen P,Malm T

Published figure using GRP78 polyclonal antibody (Product # PA1-014A) in Western Blot

Sun Jan 01 00:00:00 EST 2023

Resveratrol improves diabetes-induced cognitive dysfunction in part through the miR-146a-5p/TXNIP axis.

The Kaohsiung journal of medical sciences

Hu Y,Zhang Q,Wang JC,Wang J,Liu Y,Zhu LY,Xu JX

Published figure using GRP78 polyclonal antibody (Product # PA1-014A) in Western Blot

Sat Apr 01 00:00:00 EDT 2023

Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice.

Cellular & molecular biology letters

Qi H,Zheng Z,Liu Q

PA1-014A was used in Western Blot to investigate the roles of the CEBPB/BZW1/eIF2α axis in metabolic reprogramming and endoplasmic reticulum stress in LN.

Thu Oct 12 00:00:00 EDT 2023

Adipose tissue-specific ablation of Ces1d causes metabolic dysregulation in mice.

Life science alliance

Li G,Li X,Yang L,Wang S,Dai Y,Fekry B,Veillon L,Tan L,Berdeaux R,Eckel-Mahan K,Lorenzi PL,Zhao Z,Lehner R,Sun K

PA1-014A was used in Western Blot to reveal that adipose tissue-specific Ces1d knock-out (FKO) mice gained more body weight with increased fat mass during a high fat-diet challenge.

Mon Aug 01 00:00:00 EDT 2022

The Protection of Zinc against Acute Cadmium Exposure: A Morphological and Molecular Study on a BBB In Vitro Model.

Cells

Branca JJV,Carrino D,Paternostro F,Morucci G,Fiorillo C,Nicoletti C,Gulisano M,Ghelardini C,Di Cesare Mannelli L,Becatti M,Pacini A

PA1-014A was used in Western Blotting to demonstrate that Zn protection from the SOD activity leads to ZO-1 downregulation and in blood-brain barrier damage.

Sun May 15 00:00:00 EDT 2022

Missense PNLIP mutations impeding pancreatic lipase secretion cause protein misfolding and endoplasmic reticulum stress.

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]

Toldi V,Kassay N,Szabó A

PA1-014A was used in Western Blot to investigate whether pancreatic lipase (PNLIP) mutants with a secretion defect result in endoplasmic reticulum stress in cell culture models.

Fri Oct 01 00:00:00 EDT 2021

Dexmedetomidine Preconditioning Reduces Myocardial Ischemia-Reperfusion Injury in Rats by Inhibiting the PERK Pathway.

Arquivos brasileiros de cardiologia

Chen Y,Cao S,Chen H,Yin C,Xu X,Yang Z

Published figure using GRP78 polyclonal antibody (Product # PA1-014A) in Western Blot

Wed Dec 01 00:00:00 EST 2021

Purified EDEM3 or EDEM1 alone produces determinant oligosaccharide structures from M8B in mammalian glycoprotein ERAD.

eLife

George G,Ninagawa S,Yagi H,Furukawa JI,Hashii N,Ishii-Watabe A,Deng Y,Matsushita K,Ishikawa T,Mamahit YP,Maki Y,Kajihara Y,Kato K,Okada T,Mori K

Published figure using GRP78 polyclonal antibody (Product # PA1-014A) in Western Blot

Tue Oct 26 00:00:00 EDT 2021

Osteoblastic Swedish mutant APP expedites brain deficits by inducing endoplasmic reticulum stress-driven senescence.

Communications biology

Pan JX,Sun D,Lee D,Xiong L,Ren X,Guo HH,Yao LL,Lu Y,Jung C,Xiong WC

PA1-014A was used in Western Blot, Immunocytochemistry to show that TgAPPsweOCN mice also exhibit Alzheimer's disease-relevant brain pathologies and behavior phenotypes.

Thu Nov 25 00:00:00 EST 2021

Osteoblastic Swedish mutant APP expedites brain deficits by inducing endoplasmic reticulum stress-driven senescence.

Communications biology

Pan JX,Sun D,Lee D,Xiong L,Ren X,Guo HH,Yao LL,Lu Y,Jung C,Xiong WC

PA1-014A was used in Western Blot, Immunocytochemistry to show that TgAPPsweOCN mice also exhibit Alzheimer's disease-relevant brain pathologies and behavior phenotypes.

Thu Nov 25 00:00:00 EST 2021

Osteoblastic Swedish mutant APP expedites brain deficits by inducing endoplasmic reticulum stress-driven senescence.

Communications biology

Pan JX,Sun D,Lee D,Xiong L,Ren X,Guo HH,Yao LL,Lu Y,Jung C,Xiong WC

PA1-014A was used in Western Blot, Immunocytochemistry to show that TgAPPsweOCN mice also exhibit Alzheimer's disease-relevant brain pathologies and behavior phenotypes.

Thu Nov 25 00:00:00 EST 2021

Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage.

Antioxidants (Basel, Switzerland)

Karunarathne WAHM,Molagoda IMN,Lee KT,Choi YH,Yu SM,Kang CH,Kim GY

PA1-014A was used in Western Blotting to investigate the protective effect of AHs against UVB-induced damage.

Fri Apr 09 00:00:00 EDT 2021

Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage.

Antioxidants (Basel, Switzerland)

Karunarathne WAHM,Molagoda IMN,Lee KT,Choi YH,Yu SM,Kang CH,Kim GY

PA1-014A was used in Western Blotting to investigate the protective effect of AHs against UVB-induced damage.

Fri Apr 09 00:00:00 EDT 2021

Adenine base editing reduces misfolded protein accumulation and toxicity in alpha-1 antitrypsin deficient patient iPSC-hepatocytes.

Molecular therapy : the journal of the American Society of Gene Therapy

Werder RB,Kaserman JE,Packer MS,Lindstrom-Vautrin J,Villacorta-Martin C,Young LE,Aratyn-Schaus Y,Gregoire F,Wilson AA

PA1-014A was used in Immunohistochemistry, Flow Cytometry, and Immunocytochemistry to apply adenine base editors to correct the Z mutation, a single-base substitution that leads to AAT protein misfolding and associated liver and lung disease, in patient induced pluripotent stem cells (iPSCs) and iPSC-derived hepatocytes (iHeps).

Wed Nov 03 00:00:00 EDT 2021

Adenine base editing reduces misfolded protein accumulation and toxicity in alpha-1 antitrypsin deficient patient iPSC-hepatocytes.

Molecular therapy : the journal of the American Society of Gene Therapy

Werder RB,Kaserman JE,Packer MS,Lindstrom-Vautrin J,Villacorta-Martin C,Young LE,Aratyn-Schaus Y,Gregoire F,Wilson AA

Wed Nov 03 00:00:00 EDT 2021

Adenine base editing reduces misfolded protein accumulation and toxicity in alpha-1 antitrypsin deficient patient iPSC-hepatocytes.

Molecular therapy : the journal of the American Society of Gene Therapy

Werder RB,Kaserman JE,Packer MS,Lindstrom-Vautrin J,Villacorta-Martin C,Young LE,Aratyn-Schaus Y,Gregoire F,Wilson AA

Wed Nov 03 00:00:00 EDT 2021

Adenine base editing reduces misfolded protein accumulation and toxicity in alpha-1 antitrypsin deficient patient iPSC-hepatocytes.

Molecular therapy : the journal of the American Society of Gene Therapy

Werder RB,Kaserman JE,Packer MS,Lindstrom-Vautrin J,Villacorta-Martin C,Young LE,Aratyn-Schaus Y,Gregoire F,Wilson AA

Wed Nov 03 00:00:00 EDT 2021

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

The Journal of biological chemistry

Tsai SJ,Atai NA,Cacciottolo M,Nice J,Salehi A,Guo C,Sedgwick A,Kanagavelu S,Gould SJ

PA1-014A was used in Western Blot to describe an alternative approach using exosomes, the only biologically normal nanovesicle.

Mon Nov 01 00:00:00 EDT 2021

Novel role of dynamin-related-protein 1 in dynamics of ER-lipid droplets in adipose tissue.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li X,Yang L,Mao Z,Pan X,Zhao Y,Gu X,Eckel-Mahan K,Zuo Z,Tong Q,Hartig SM,Cheng X,Du G,Moore DD,Bellen HJ,Sesaki H,Sun K

PA1-014A was used in Western Blotting to report that DRP1 translocates to endoplasmic reticulum (ER) in response to β-adrenergic stimulation.

Mon Jun 01 00:00:00 EDT 2020

Omega-3 Fatty Acid-Enriched Fish Oil and Selenium Combination Modulates Endoplasmic Reticulum Stress Response Elements and Reverses Acquired Gefitinib Resistance in HCC827 Lung Adenocarcinoma Cells.

Marine drugs

Liao CH,Tzeng YT,Lai GM,Chang CL,Hu MH,Tsai WL,Liu YR,Hsia S,Chuang SE,Chiou TJ,Wang LM,Whang-Peng J,Yao CJ

PA1-014A was used in Western Blotting to suggest the potential of combining FO and Se in relieving the acquired resistance of NSCLC patients to targeted therapy.

Wed Jul 29 00:00:00 EDT 2020

Omega-3 Fatty Acid-Enriched Fish Oil and Selenium Combination Modulates Endoplasmic Reticulum Stress Response Elements and Reverses Acquired Gefitinib Resistance in HCC827 Lung Adenocarcinoma Cells.

Marine drugs

Liao CH,Tzeng YT,Lai GM,Chang CL,Hu MH,Tsai WL,Liu YR,Hsia S,Chuang SE,Chiou TJ,Wang LM,Whang-Peng J,Yao CJ

PA1-014A was used in Western Blotting to suggest the potential of combining FO and Se in relieving the acquired resistance of NSCLC patients to targeted therapy.

Wed Jul 29 00:00:00 EDT 2020

Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification.

Nature communications

Santos MCFD,Anderson CP,Neschen S,Zumbrennen-Bullough KB,Romney SJ,Kahle-Stephan M,Rathkolb B,Gailus-Durner V,Fuchs H,Wolf E,Rozman J,de Angelis MH,Cai WM,Rajan M,Hu J,Dedon PC,Leibold EA

Published figure using GRP78 polyclonal antibody (Product # PA1-014A) in Western Blot

Wed Jan 15 00:00:00 EST 2020

A mechanical acupuncture instrument mitigates the endoplasmic reticulum stress and oxidative stress of ovariectomized rats.

Integrative medicine research

Seo SY,Kang SY,Kwon OS,Bang SK,Kim SP,Choi KH,Moon JY,Ryu Y

PA1-014A was used in Immunohistochemistry-immunofluorescence to investigate whether stimulation of Sanyinjiao using a mechanical acupuncture instrument can alleviate depression-like behaviour caused by estrogen deficiency in OVX rats.

Sun Sep 01 00:00:00 EDT 2019

GRP78 translocation to the cell surface and O-GlcNAcylation of VE-Cadherin contribute to ER stress-mediated endothelial permeability.

Scientific reports

Lenin R,Nagy PG,Jha KA,Gangaraju R

Published figure using GRP78 polyclonal antibody (Product # PA1-014A) in Immunocytochemistry

Thu Jul 25 00:00:00 EDT 2019

GRP78 translocation to the cell surface and O-GlcNAcylation of VE-Cadherin contribute to ER stress-mediated endothelial permeability.

Scientific reports

Lenin R,Nagy PG,Jha KA,Gangaraju R

Published figure using GRP78 polyclonal antibody (Product # PA1-014A) in Immunocytochemistry

Thu Jul 25 00:00:00 EDT 2019

Proteomic Analysis of Placental Mitochondria Following Trophoblast Differentiation.

Frontiers in physiology

Fisher JJ,McKeating DR,Cuffe JS,Bianco-Miotto T,Holland OJ,Perkins AV

PA1-014A was used in Western Blotting to show proteomic shifts in placental mitochondrial metabolism and dynamics post-trophoblast differentiation.

Wed Sep 30 00:00:00 EDT 2020

Featured Article: Deterioration of visual function mediated by senescence-associated endoplasmic reticulum stress in inflammatory tie2-TNF mice.

Experimental biology and medicine (Maywood, N.J.)

Lenin R,Nagy PG,Gentry J,Gangaraju R

PA1-014A was used in Western Blotting to investigate the relationship between endothelial dysfunction, endoplasmic reticulum (ER) stress, and cellular senescence in the development of retinal dysfunction.

Wed Aug 01 00:00:00 EDT 2018

Depletion of the membrane-fusion regulator Munc18c attenuates caerulein hyperstimulation-induced pancreatitis.

The Journal of biological chemistry

Dolai S,Liang T,Orabi AI,Xie L,Holmyard D,Javed TA,Fernandez NA,Xie H,Cattral MS,Thurmond DC,Thorn P,Gaisano HY

Published figure using GRP78 polyclonal antibody (Product # PA1-014A) in Western Blot

Fri Feb 16 00:00:00 EST 2018

Opposite Regulation of CHOP and GRP78 and Synergistic Apoptosis Induction by Selenium Yeast and Fish Oil via AMPK Activation in Lung Adenocarcinoma Cells.

Nutrients

Kao RH,Lai GM,Chow JM,Liao CH,Zheng YM,Tsai WL,Hsia S,Lai IC,Lee HL,Chuang SE,Whang-Peng J,Yao CJ

PA1-014A was used in Western Blotting to investigate the effects of selenium yeast and fish oil on apoptosis induction in lung adenocarcinoma cells, and elucidate the underlying mechanism.

Mon Oct 08 00:00:00 EDT 2018

NCOA3 coactivator is a transcriptional target of XBP1 and regulates PERK-eIF2α-ATF4 signalling in breast cancer.

Oncogene

Gupta A,Hossain MM,Miller N,Kerin M,Callagy G,Gupta S

PA1-014A was used in Western Blotting to identify XBP1-NCOA3 axis that regulates cell fate decisions in ER-positive breast cancer cells.

Thu Nov 10 00:00:00 EST 2016

A mouse model suggests two mechanisms for thyroid alterations in infantile cystinosis: decreased thyroglobulin synthesis due to endoplasmic reticulum stress/unfolded protein response and impaired lysosomal processing.

Endocrinology

Gaide Chevronnay HP,Janssens V,Van Der Smissen P,Liao XH,Abid Y,Nevo N,Antignac C,Refetoff S,Cherqui S,Pierreux CE,Courtoy PJ

PA1-014A was used in Western Blotting to define early alterations in cystinosin knockout mice, and identify subcellular and molecular mechanisms.

Mon Jun 01 00:00:00 EDT 2015

Endocrinology

Gaide Chevronnay HP,Janssens V,Van Der Smissen P,Liao XH,Abid Y,Nevo N,Antignac C,Refetoff S,Cherqui S,Pierreux CE,Courtoy PJ

PA1-014A was used in Western Blotting to define early alterations in cystinosin knockout mice, and identify subcellular and molecular mechanisms.

Mon Jun 01 00:00:00 EDT 2015

PERK regulated miR-424(322)-503 cluster fine-tunes activation of IRE1 and ATF6 during Unfolded Protein Response.

Scientific reports

Gupta A,Hossain MM,Read DE,Hetz C,Samali A,Gupta S

PA1-014A was used in western blot to study the miR-424(322)-503 cluster in the unfolded protein response

Thu Dec 17 00:00:00 EST 2015

PERK regulated miR-424(322)-503 cluster fine-tunes activation of IRE1 and ATF6 during Unfolded Protein Response.

Scientific reports

Gupta A,Hossain MM,Read DE,Hetz C,Samali A,Gupta S

PA1-014A was used in western blot to study the miR-424(322)-503 cluster in the unfolded protein response

Thu Dec 17 00:00:00 EST 2015

Analysis of the membrane proteome of ciprofloxacin-resistant macrophages by stable isotope labeling with amino acids in cell culture (SILAC).

PloS one

Caceres NE,Aerts M,Marquez B,Mingeot-Leclercq MP,Tulkens PM,Devreese B,Van Bambeke F

Published figure using GRP78 polyclonal antibody (Product # PA1-014A) in Western Blot

Fri Sep 06 00:00:00 EDT 2013

Proteomic analysis of α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor complexes.

The Journal of biological chemistry

Kang MG,Nuriya M,Guo Y,Martindale KD,Lee DZ,Huganir RL

PA1-014A was used in western blot to identify novel components of brain AMPA receptors using a proteomic approach

Fri Aug 17 00:00:00 EDT 2012

Dysferlin overexpression in skeletal muscle produces a progressive myopathy.

Annals of neurology

Glover LE,Newton K,Krishnan G,Bronson R,Boyle A,Krivickas LS,Brown RH

PA1-014A was used in western blot to characterize the effect of dysferlin level on myopathy

Mon Mar 01 00:00:00 EST 2010

Ras inhibits endoplasmic reticulum stress in human cancer cells with amplified Myc.

International journal of cancer

Yaari-Stark S,Shaked M,Nevo-Caspi Y,Jacob-Hircsh J,Shamir R,Rechavi G,Kloog Y

PA1-014A was used in western blot to study the effect of Ras on endoplasmic reticulum stress in human cancer cells and its mechanism

Sat May 15 00:00:00 EDT 2010

HtrA2 regulates beta-amyloid precursor protein (APP) metabolism through endoplasmic reticulum-associated degradation.

The Journal of biological chemistry

Huttunen HJ,Guénette SY,Peach C,Greco C,Xia W,Kim DY,Barren C,Tanzi RE,Kovacs DM

PA1-014A was used in western blot to study the effect of HtrA2 on beta amyloid precursor protein metabolism.

Fri Sep 21 00:00:00 EDT 2007

Identification of mRNA/protein (mRNP) complexes containing Puralpha, mStaufen, fragile X protein, and myosin Va and their association with rough endoplasmic reticulum equipped with a kinesin motor.

The Journal of biological chemistry

Ohashi S,Koike K,Omori A,Ichinose S,Ohara S,Kobayashi S,Sato TA,Anzai K

PA1-014A was used in western blot to investigate the function of the rough endoplasmic reticulum structure during the transport of polyribosomes.

Fri Oct 04 00:00:00 EDT 2002

Response of neurons to an irreversible inhibition of endoplasmic reticulum Ca(2+)-ATPase: relationship between global protein synthesis and expression and translation of individual genes.

The Biochemical journal

Mengesdorf T,Althausen S,Oberndorfer I,Paschen W

PA1-014A was used in western blot to investigate Tg-induced inhibition and recovery of protein synthesis and corresponding changes of particular genes in mRNA level and protein level

Fri Jun 15 00:00:00 EDT 2001

Membrane topology of the murine fatty acid transport protein 1.

The Journal of biological chemistry

Lewis SE,Listenberger LL,Ory DS,Schaffer JE

PA1-014A was used in immunocytochemistry to study the topology of murine fatty acid transport protein

Fri Oct 05 00:00:00 EDT 2001

Bestrophin, the product of the Best vitelliform macular dystrophy gene (VMD2), localizes to the basolateral plasma membrane of the retinal pigment epithelium.

Proceedings of the National Academy of Sciences of the United States of America

Marmorstein AD,Marmorstein LY,Rayborn M,Wang X,Hollyfield JG,Petrukhin K

PA1-014A was used in immunoprecipitation to investigate the localization of bestrophin in rat retinal pigment epithelium.

Tue Nov 07 00:00:00 EST 2000

Developmental regulation of FKBP65. An ER-localized extracellular matrix binding-protein.

Molecular biology of the cell

Patterson CE,Schaub T,Coleman EJ,Davis EC

PA1-014A was used in western blot to investigate the regulation of FKBP65 subcellular localization and expression level during organism development.

Wed Nov 01 00:00:00 EST 2000

Bioinformatics

Protein Aliases: 78 kDa glucose-regulated protein; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; FLJ26106; glucose regulated protein, 78 kDa; glucose-regulated protein, 78kDa; GRP 78; GRP-78; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; Heat shock protein family A member 5; HSP70 family protein 5; HSPA 5; Immunoglobulin heavy chain-binding protein; Steroidogenesis-activator polypeptide; XAP-1 antigen

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Gene Aliases: AL022860; AU019543; baffled; BIP; D2Wsu141e; D2Wsu17e; GRP78; HEL-S-89n; Hsce70; HSPA5; mBiP; MIF2; SEZ-7; Sez7

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UniProt ID: (Human) P11021, (Rat) P06761, (Mouse) P20029

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Entrez Gene ID: (Dog) 480726, (Human) 3309, (Rat) 25617, (Mouse) 14828

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Function(s)

glycoprotein binding calcium ion binding protein binding ATP binding ATPase activity enzyme binding protein domain specific binding ubiquitin protein ligase binding ribosome binding unfolded protein binding chaperone binding misfolded protein binding cadherin binding involved in cell-cell adhesion nucleotide binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GRP78 Polyclonal Antibody

GRP78 Antibody (PA1-014A) in ICC/IF

Product Details

PA1-014A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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