Invitrogen

GRP78 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

13 References

View all (63) GRP78 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GRP78 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

GRP78 Antibody (PA5-19503) in IHC (P)

Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Liver Carcinoma tissue using Product # PA5-19503, anti-GRP78 BiP antibody. Primary antibody was used at a concentration of 1 µg/mL and exposed for 8 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6/20mins). The detection method was a HRP conjugated polymer, DAB chromoge... View More

GRP78 Antibody in Immunocytochemistry (ICC/IF)

GRP78 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GRP78 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using GRP78 Polyclonal Antibody (Product # PA519503), shows increased expression of proteins in HeLa and NIH/3T3 upon Thapsigargin treatment. {TM}

Product Details

PA5-19503

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-5 µg/ml

View 3 publications 3 publications

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Mouse GRP78 BiP.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978031

Product Specific Information

For Western Blot, this antibody has non-specific bands at 100 kDa.

Heat mediated antigen retrieval recommended prior to tissue staining.

Store antibody at 4ºC for 1-2 weeks. For long-term storage, store at -20ºC.

Target Information

GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78’s involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Polarized Desmosome and Hemidesmosome Shedding via Small Extracellular Vesicles is an Early Indicator of Outer Blood-Retina Barrier Dysfunction.

Journal of extracellular biology

Hernandez BJ,Skiba NP,Plössl K,Strain M,Liu Y,Grigsby D,Kelly U,Cady MA,Manocha V,Maminishkis A,Watkins T,Miller SS,Ashley-Koch A,Stamer WD,Weber BHF,Bowes Rickman C,Klingeborn M

Published figure using GRP78 polyclonal antibody (Product # PA5-19503) in Western Blot

Sun Oct 01 00:00:00 EDT 2023

Enhancing calmodulin binding to ryanodine receptor is crucial to limit neuronal cell loss in Alzheimer disease.

Scientific reports

Nakamura Y,Yamamoto T,Xu X,Kobayashi S,Tanaka S,Tamitani M,Saito T,Saido TC,Yano M

PA5-19503 was used in Immunocytochemistry to investigate (1) the pathogenic role of destabilization of ryanodine receptor (RyR2) in endoplasmic reticulum (ER) upon development of AD phenotypes in AppNL-G-F mice, which harbor three familial AD mutations (Swedish, Beyreuther/Iberian, and Arctic), and (2) the therapeutic effect of enhanced calmodulin (CaM) binding to RyR2.

Wed Mar 31 00:00:00 EDT 2021

Enhancing calmodulin binding to ryanodine receptor is crucial to limit neuronal cell loss in Alzheimer disease.

Scientific reports

Nakamura Y,Yamamoto T,Xu X,Kobayashi S,Tanaka S,Tamitani M,Saito T,Saido TC,Yano M

Wed Mar 31 00:00:00 EDT 2021

The role of vaspin in porcine corpus luteum.

The Journal of endocrinology

Kurowska P,Mlyczyńska E,Dawid M,Grzesiak M,Dupont J,Rak A

PA5-19503 was used in Western Blot, Immunohistochemistry to find that vaspin/GRP78 expression depends on the luteal phase stage and vaspin affects luteal cells endocrinology, indicating that vaspin is a new regulator of luteal cells steroidogenesis and CL formation.

Tue Dec 01 00:00:00 EST 2020

The role of vaspin in porcine corpus luteum.

The Journal of endocrinology

Kurowska P,Mlyczyńska E,Dawid M,Grzesiak M,Dupont J,Rak A

Tue Dec 01 00:00:00 EST 2020

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.

International journal of molecular sciences

Kurowska P,Mlyczyńska E,Estienne A,Barbe A,Rajska I,Soból K,Poniedziałek-Kempny K,Dupont J,Rak A

PA5-19503 was used in Western Blot, Immunocytochemistry to show that vaspin, by improving in vitro oocyte maturation via MAP3/1 and PRKAA1 kinase pathways, can be a new factor to improve in vitro fertilisation protocols.

Tue Dec 08 00:00:00 EST 2020

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.

International journal of molecular sciences

Kurowska P,Mlyczyńska E,Estienne A,Barbe A,Rajska I,Soból K,Poniedziałek-Kempny K,Dupont J,Rak A

Tue Dec 08 00:00:00 EST 2020

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.

International journal of molecular sciences

Kurowska P,Mlyczyńska E,Estienne A,Barbe A,Rajska I,Soból K,Poniedziałek-Kempny K,Dupont J,Rak A

Tue Dec 08 00:00:00 EST 2020

Effect of vitamin B₁₂ supplementation on retinal lesions in diabetic rats.

Molecular vision

Reddy SS,Prabhakar YK,Kumar CU,Reddy PY,Reddy GB

PA5-19503 was used in Western Blotting to investigate the impact of dietary supplementation of vitamin B12 on retinal changes in diabetic rats.

Mon May 10 00:00:00 EDT 2021

Effect of vitamin B₁₂ supplementation on retinal lesions in diabetic rats.

Molecular vision

Reddy SS,Prabhakar YK,Kumar CU,Reddy PY,Reddy GB

PA5-19503 was used in Western Blotting to investigate the impact of dietary supplementation of vitamin B12 on retinal changes in diabetic rats.

Mon May 10 00:00:00 EDT 2021

Dantrolene prevents hepatic steatosis by reducing cytoplasmic Ca²⁺ level and ER stress.

Biochemistry and biophysics reports

Tamitani M,Yamamoto T,Yamamoto N,Fujisawa K,Tanaka S,Nakamura Y,Uchinoumi H,Oda T,Okuda S,Takami T,Kobayashi S,Sakaida I,Yano M

PA5-19503 was used in Immunohistochemistry to propose that dantrolene is a potential new therapeutic agent against fatty liver disease.

Tue Sep 01 00:00:00 EDT 2020

Dantrolene prevents hepatic steatosis by reducing cytoplasmic Ca²⁺ level and ER stress.

Biochemistry and biophysics reports

Tamitani M,Yamamoto T,Yamamoto N,Fujisawa K,Tanaka S,Nakamura Y,Uchinoumi H,Oda T,Okuda S,Takami T,Kobayashi S,Sakaida I,Yano M

PA5-19503 was used in Immunohistochemistry to propose that dantrolene is a potential new therapeutic agent against fatty liver disease.

Tue Sep 01 00:00:00 EDT 2020

Flutamide Alters the Expression of Chemerin, Apelin, and Vaspin and Their Respective Receptors in the Testes of Adult Rats.

International journal of molecular sciences

Brzoskwinia M,Pardyak L,Rak A,Kaminska A,Hejmej A,Marek S,Kotula-Balak M,Bilinska B

PA5-19503 was used in Western Blot, Immunohistochemistry (Paraffin) to study the impact of short-term exposure to flutamide on the expression of chemerin, apelin, vaspin and their receptors in rat testes.

Mon Jun 22 00:00:00 EDT 2020

Flutamide Alters the Expression of Chemerin, Apelin, and Vaspin and Their Respective Receptors in the Testes of Adult Rats.

International journal of molecular sciences

Brzoskwinia M,Pardyak L,Rak A,Kaminska A,Hejmej A,Marek S,Kotula-Balak M,Bilinska B

Mon Jun 22 00:00:00 EDT 2020

Flutamide Alters the Expression of Chemerin, Apelin, and Vaspin and Their Respective Receptors in the Testes of Adult Rats.

International journal of molecular sciences

Brzoskwinia M,Pardyak L,Rak A,Kaminska A,Hejmej A,Marek S,Kotula-Balak M,Bilinska B

Mon Jun 22 00:00:00 EDT 2020

Flutamide Alters the Expression of Chemerin, Apelin, and Vaspin and Their Respective Receptors in the Testes of Adult Rats.

International journal of molecular sciences

Brzoskwinia M,Pardyak L,Rak A,Kaminska A,Hejmej A,Marek S,Kotula-Balak M,Bilinska B

Mon Jun 22 00:00:00 EDT 2020

Ubiquitin-proteasome system and ER stress in the brain of diabetic rats.

Journal of cellular biochemistry

Shruthi K,Reddy SS,Chitra PS,Reddy GB

PA5-19503 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that altered ubiquitin-proteasome system could be an underlying mechanism of neuronal apoptosis in diabetes and examine the therapeutic effect of 4-phenylbutyric acid (4-PBA), an ER stress inhibitor.

Mon Apr 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: 78 kDa glucose-regulated protein; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; FLJ26106; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein, 78kDa; GRP 78; GRP-78; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 5; Heat shock protein 70 family protein 5; Heat shock protein family A member 5; HSP70 family protein 5; HSPA 5; Immunoglobulin heavy chain-binding protein; Steroidogenesis-activator polypeptide; XAP-1 antigen

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Gene Aliases: AL022860; AU019543; baffled; BIP; D2Wsu141e; D2Wsu17e; GRP78; HEL-S-89n; Hsce70; HSPA5; I79_019946; mBiP; MIF2; SEZ-7; Sez7

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UniProt ID: (Human) P11021, (Rat) P06761, (Mouse) P20029, (Chinese hamster) G3I8R9

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Entrez Gene ID: (Human) 3309, (Rat) 25617, (Mouse) 14828, (Chinese hamster) 100689305

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Function(s)

glycoprotein binding calcium ion binding protein binding ATP binding ATPase activity enzyme binding protein domain specific binding ubiquitin protein ligase binding ribosome binding unfolded protein binding chaperone binding misfolded protein binding cadherin binding involved in cell-cell adhesion nucleotide binding

Process(es)

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GRP78 Polyclonal Antibody

GRP78 Antibody (PA5-19503) in IHC (P)

Product Details

PA5-19503

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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