Invitrogen

GRP94 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

6 References

View all (34) GRP94 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GRP94 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

GRP94 Antibody (36-2600) in ICC/IF

Immunofluorescence analysis of GRP94 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labelled with GRP94 Polyclonal Antibody (Product # 36-2600) at 5µg/mL in 0.1% BSA and incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 555 conjugate (Product # 36-2600)... View More

GRP94 Antibody in Immunohistochemistry (IHC)

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GRP94 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-87 MG cells were transfected with GRP94 siRNA and loss of signal was observed in Western Blot using Anti-GRP94 polyclonal Antibody (Product # 36-2600). {KD}

Product Details

36-2600

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

5 µg/mL

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the N-terminal region of the mouse and human gp96 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533246

Target Information

GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Control of cell surface expression of GABA_A receptors by a conserved region at the end of the N-terminal extracellular domain of receptor subunits.

The Journal of biological chemistry

Yuan B,Hatchett-Walker C,Long P,Xu Z,Stephenson FA,Haider S,Jovanovic JN

Published figure using GRP94 polyclonal antibody (Product # 36-2600) in Western Blot

Thu Dec 01 00:00:00 EST 2022

Small Extracellular Vesicles from Head and Neck Squamous Cell Carcinoma Cells Carry a Proteomic Signature for Tumor Hypoxia.

Cancers

Głuszko A,Szczepański MJ,Whiteside TL,Reichert TE,Siewiera J,Ludwig N

Published figure using GRP94 polyclonal antibody (Product # 36-2600) in Western Blot

Thu Aug 19 00:00:00 EDT 2021

Activation of (pro)renin by (pro)renin receptor in extracellular vesicles from osteoclasts.

Scientific reports

Murray JB,Mikhael C,Han G,de Faria LP,Rody WJ,Holliday LS

Published figure using GRP94 polyclonal antibody (Product # 36-2600) in Western Blot

Wed Apr 28 00:00:00 EDT 2021

Grp94 Regulates the Recruitment of Aneural AChR Clusters for the Assembly of Postsynaptic Specializations by Modulating ADF/Cofilin Activity and Turnover.

eNeuro

Chan ZC,Deng L,Lee CW

36-2600 was used in Western Blotting to investigatE the involvement of heat shock protein 90 (HSP90) family of stress proteins in neuromuscular junctions (NMJ) development.

Fri Jun 18 00:00:00 EDT 2021

Grp94 Regulates the Recruitment of Aneural AChR Clusters for the Assembly of Postsynaptic Specializations by Modulating ADF/Cofilin Activity and Turnover.

eNeuro

Chan ZC,Deng L,Lee CW

36-2600 was used in Western Blotting to investigatE the involvement of heat shock protein 90 (HSP90) family of stress proteins in neuromuscular junctions (NMJ) development.

Fri Jun 18 00:00:00 EDT 2021

Characterization of Regulatory Extracellular Vesicles from Osteoclasts.

Journal of dental research

Huynh N,VonMoss L,Smith D,Rahman I,Felemban MF,Zuo J,Rody WJ,McHugh KP,Holliday LS

36-2600 was used in western blot to analyze osteoclasts and its regulatory extracellular vesicles

Wed Jun 01 00:00:00 EDT 2016

Inhibitors of cathepsins B and L induce autophagy and cell death in neuroblastoma cells.

Investigational new drugs

Cartledge DM,Colella R,Glazewski L,Lu G,Mason RW

Fri Feb 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; ERp99; gp96 homolog; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family C member 4; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Polymorphic tumor rejection antigen 1; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96

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Gene Aliases: ECGP; endoplasmin; ERp99; GP96; GRP94; HEL-S-125m; HEL35; HSP90B1; HSPC4; TA-3; Targ2; Tra-1; TRA1

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UniProt ID: (Human) P14625, (Mouse) P08113, (Rat) Q66HD0

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Entrez Gene ID: (Human) 7184, (Mouse) 22027, (Rat) 362862

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Function(s)

RNA binding calcium ion binding protein binding ATP binding protein phosphatase binding virion binding low-density lipoprotein particle receptor binding unfolded protein binding nucleotide binding Hsp90 family chaperone

Process(es)

response to hypoxia toll-like receptor signaling pathway receptor-mediated endocytosis protein transport ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol actin rod assembly protein folding in endoplasmic reticulum response to endoplasmic reticulum stress ATF6-mediated unfolded protein response negative regulation of apoptotic process regulation of phosphoprotein phosphatase activity sequestering of calcium ion cellular response to ATP protein folding response to stress

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GRP94 Polyclonal Antibody

GRP94 Antibody (36-2600) in ICC/IF

Product Details

36-2600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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