Invitrogen

GRP94 Monoclonal Antibody (9G10)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4 Published Figures

21 References

View all (34) GRP94 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GRP94 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

GRP94 Antibody (MA3-016) in ICC/IF

Immunofluorescent analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) (Product # MA3-016) shows staining in C6 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 (Product # MA3-016) at a dilution of 1:100 over night at 4ºC, washed with PBS and ... View More

GRP94 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GRP94 Antibody — The specificity of anti-GRP94 monoclonal antibody (Product # MA3-016) was demonstrated by CRISPR targeted GRP94 knockout in HEK293cells. Western blot analysis of whole cell lysates using this antibody showed no detection of GRP94 protein expression in GRP94 knockout 293 cells (lane 2) compared to wildtype cells (lane 1). {KO}

Product Details

MA3-016

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:250

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

View 4 publications 4 publications

Product Specifications

Species Reactivity

Bovine, Chicken, Hamster, Human, Mouse, Rat

Published species

Chicken, Hamster, Human, Mouse, Pig, Rat, Xenopus

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

9G10

Immunogen

Chick oviduct GRP94.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2248666

Product Specific Information

MA3-016 detects glucose regulated protein 94 kDa (GRP94) from human, rat, mouse, chick, hamster and bovine tissues. MA3-016 is also expected to react with GRP94 from Xenopus, human, canine, rabbit, non-human primate, canine, Drosophila, and ovine tissues.

MA3-016 has been successfully used in Western blot, Immunohistochemistry (paraffin), immunocytochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~108 kDa protein representing GRP94 in chick oviduct cytosol.

The MA3-016 immunogen is chick oviduct GRP94.

Target Information

GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization.

Nature

Qian K,Tol MJ,Wu J,Uchiyama LF,Xiao X,Cui L,Bedard AH,Weston TA,Rajendran PS,Vergnes L,Shimanaka Y,Yin Y,Jami-Alahmadi Y,Cohn W,Bajar BT,Lin CH,Jin B,DeNardo LA,Black DL,Whitelegge JP,Wohlschlegel JA,Reue K,Shivkumar K,Chen FJ,Young SG,Li P,Tontonoz P

MA3-016 was used in Western Blot to define a molecular mechanism that regulates lipid droplet form and function to facilitate lipid utilization in thermogenic adipocytes.

Sun Jan 01 00:00:00 EST 2023

The Plasma Membrane Monoamine Transporter is Highly Expressed in Neuroblastoma and Functions as an mIBG Transporter.

The Journal of pharmacology and experimental therapeutics

Vieira LS,Zhang Y,López Quiñones AJ,Hu T,Singh DK,Stevens J,Prasad B,Park JR,Wang J

MA3-016 was used in Immunocytochemistry-immunofluorescence to suggest that PMAT is a previously unrecognized transporter highly expressed in NB and could impact intracellular transport and therapeutic response to 131I-mIBG.

Fri Dec 01 00:00:00 EST 2023

Cooperative treatment effectiveness of ATR and HSP90 inhibition in Ewing's sarcoma cells.

Cell & bioscience

Marx C,Schaarschmidt MU,Kirkpatrick J,Marx-Blümel L,Halilovic M,Westermann M,Hoelzer D,Meyer FB,Geng Y,Buder K,Schadwinkel HM,Siniuk K,Becker S,Thierbach R,Beck JF,Sonnemann J,Wang ZQ

Published figure using GRP94 monoclonal antibody (Product # MA3-016) in Immunocytochemistry

Sat Mar 20 00:00:00 EDT 2021

The inducible β5i proteasome subunit contributes to proinsulin degradation in GRP94-deficient β-cells and is overexpressed in type 2 diabetes pancreatic islets.

American journal of physiology. Endocrinology and metabolism

Khilji MS,Bresson SE,Verstappen D,Pihl C,Andersen PAK,Agergaard JB,Dahlby T,Bryde TH,Klindt K,Nielsen CK,Walentinsson A,Zivkovic D,Bousquet MP,Tyrberg B,Richardson SJ,Morgan NG,Mandrup-Poulsen T,Marzec MT

MA3-016 was used in Western Blotting to investigate the role of different proteasomes in proinsulin degradation, in particular the inducible β5i proteasome subunit and its role in the degradation of mishandled proinsulin.

Mon Jun 01 00:00:00 EDT 2020

Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivo.

Journal of extracellular vesicles

Elashiry M,Elashiry MM,Elsayed R,Rajendran M,Auersvald C,Zeitoun R,Rashid MH,Ara R,Meghil MM,Liu Y,Arbab AS,Arce RM,Hamrick M,Elsalanty M,Brendan M,Pacholczyk R,Cutler CW

MA3-016 was used in Western Blotting to examine the immunobiology of dendritic cell exosomes in mice, and their ability to reprogram immune cells responsible for experimental alveolar bone loss in vivo.

Fri Aug 07 00:00:00 EDT 2020

Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivo.

Journal of extracellular vesicles

Elashiry M,Elashiry MM,Elsayed R,Rajendran M,Auersvald C,Zeitoun R,Rashid MH,Ara R,Meghil MM,Liu Y,Arbab AS,Arce RM,Hamrick M,Elsalanty M,Brendan M,Pacholczyk R,Cutler CW

Fri Aug 07 00:00:00 EDT 2020

Cytosolic CD38 protein forms intact disulfides and is active in elevating intracellular cyclic ADP-ribose.

The Journal of biological chemistry

Zhao YJ,Zhang HM,Lam CM,Hao Q,Lee HC

MA3-016 was used in immunocytochemistry to study the effect of cytosolic CD38 protein on intracellular cyclic ADP-ribose level

Fri Jun 24 00:00:00 EDT 2011

Cytosolic CD38 protein forms intact disulfides and is active in elevating intracellular cyclic ADP-ribose.

The Journal of biological chemistry

Zhao YJ,Zhang HM,Lam CM,Hao Q,Lee HC

MA3-016 was used in immunocytochemistry to study the effect of cytosolic CD38 protein on intracellular cyclic ADP-ribose level

Fri Jun 24 00:00:00 EDT 2011

Cytosolic CD38 protein forms intact disulfides and is active in elevating intracellular cyclic ADP-ribose.

The Journal of biological chemistry

Zhao YJ,Zhang HM,Lam CM,Hao Q,Lee HC

MA3-016 was used in immunocytochemistry to study the effect of cytosolic CD38 protein on intracellular cyclic ADP-ribose level

Fri Jun 24 00:00:00 EDT 2011

Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death.

The EMBO journal

Panaretakis T,Kepp O,Brockmeier U,Tesniere A,Bjorklund AC,Chapman DC,Durchschlag M,Joza N,Pierron G,van Endert P,Yuan J,Zitvogel L,Madeo F,Williams DB,Kroemer G

MA3-016 was used in western blot to investigate the mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death

Wed Mar 04 00:00:00 EST 2009

Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.

Molecular & cellular proteomics : MCP

Gramolini AO,Kislinger T,Alikhani-Koopaei R,Fong V,Thompson NJ,Isserlin R,Sharma P,Oudit GY,Trivieri MG,Fagan A,Kannan A,Higgins DG,Huedig H,Hess G,Arab S,Seidman JG,Seidman CE,Frey B,Perry M,Backx PH,Liu PP,MacLennan DH,Emili A

MA3-016 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse

Sat Mar 01 00:00:00 EST 2008

Influence of Ki-ras-driven oncogenic transformation on the protein network of murine fibroblasts.

Proteomics

Recktenwald CV,Mendler S,Lichtenfels R,Kellner R,Seliger B

MA3-016 was used in western blot to investigate the influence of Ki-ras on protein expression in murine fibroblasts

Thu Feb 01 00:00:00 EST 2007

Relation of the size and intracellular sorting of apoB to the formation of VLDL 1 and VLDL 2.

Journal of lipid research

Stillemark-Billton P,Beck C,Borén J,Olofsson SO

MA3-016 was used in western blot to compare the two-step process and an apoB size-dependent lipidation process as to the production of different lipoproteins

Sat Jan 01 00:00:00 EST 2005

Xenopus as an experimental model for studying evolution of hsp--immune system interactions.

Methods (San Diego, Calif.)

Robert J,Gantress J,Cohen N,Maniero GD

MA3-016 was used in immunoprecipitation to investigate Xenopus as an experimental model for studying evolution of hsp--immune system interactions

Thu Jan 01 00:00:00 EST 2004

Identification of the N-terminal peptide binding site of glucose-regulated protein 94.

The Journal of biological chemistry

Gidalevitz T,Biswas C,Ding H,Schneidman-Duhovny D,Wolfson HJ,Stevens F,Radford S,Argon Y

MA3-016 was used in immunoprecipitation to investigate the N-terminal peptide binding site of glucose-regulated protein 94

Fri Apr 16 00:00:00 EDT 2004

Interaction of neuropeptide Y and Hsp90 through a novel peptide binding region.

Biochemistry

Ishiwatari-Hayasaka H,Maruya M,Sreedhar AS,Nemoto TK,Csermely P,Yahara I

MA3-016 was used in immunoprecipitation to study the mechanism for the interaction of neuropeptide Y and Hsp90

Tue Nov 11 00:00:00 EST 2003

Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane.

Reproductive biology and endocrinology : RB&E

Calvert ME,Digilio LC,Herr JC,Coonrod SA

MA3-016 was used in immunohistochemistry to identify highly abundant heat shock proteins and chaperone proteins in mature mouse egg proteome.

Fri Feb 14 00:00:00 EST 2003

Response of neurons to an irreversible inhibition of endoplasmic reticulum Ca(2+)-ATPase: relationship between global protein synthesis and expression and translation of individual genes.

The Biochemical journal

Mengesdorf T,Althausen S,Oberndorfer I,Paschen W

MA3-016 was used in western blot to investigate Tg-induced inhibition and recovery of protein synthesis and corresponding changes of particular genes in mRNA level and protein level

Fri Jun 15 00:00:00 EDT 2001

Glycosylation increases potassium channel stability and surface expression in mammalian cells.

The Journal of biological chemistry

Khanna R,Myers MP,Lainé M,Papazian DM

MA3-016 was used in immunocytochemistry to demonstrate the effect of glycosylation on Shaker protein stability and surface expression in mammalian cells.

Fri Sep 07 00:00:00 EDT 2001

Two compartments for insulin-stimulated exocytosis in 3T3-L1 adipocytes defined by endogenous ACRP30 and GLUT4.

The Journal of cell biology

Bogan JS,Lodish HF

MA3-016 was used in immunocytochemistry to study the mechanism for the insulin-regulated exocytosis in 3T3-L1 adipocytes.

Mon Aug 09 00:00:00 EDT 1999

Folding of rabies virus glycoprotein: epitope acquisition and interaction with endoplasmic reticulum chaperones.

Journal of virology

Gaudin Y

MA3-016 was used in western blot to study how rabies virus G folds in vivo

Thu May 01 00:00:00 EDT 1997

Calreticulin functions as a molecular chaperone in the biosynthesis of myeloperoxidase.

The Journal of biological chemistry

Nauseef WM,McCormick SJ,Clark RA

MA3-016 was used in immunoprecipitation to investigate the interaction between calreticulin and glycosylated apopro-myeloperoxidase

Fri Mar 03 00:00:00 EST 1995

Structural analysis of chicken oviduct progesterone receptor using monoclonal antibodies to the subunit B protein.

Biochemistry

Edwards DP,Weigel NL,Schrader WT,O'Malley BW,McGuire WL

MA3-016 was used in western blot to assess the structure of chicken oviduct progesterone receptor

Tue Sep 11 00:00:00 EDT 1984

Bioinformatics

Protein Aliases: 108K heat shock protein; 108K HSP; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; ERp99; gp96 homolog; GRP-94; GRP94; Heat shock 108 kDa protein; heat shock protein 108; heat shock protein 108.; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90, beta (Grp94), member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; heat shock protein 90kDa beta, member 1; Heat shock protein family C member 4; HSP 108; hsp108; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Polymorphic tumor rejection antigen 1; stress-inducible tumor rejection antigen gp96; Transferrin-binding protein; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96

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Gene Aliases: ECGP; endoplasmin; ERp99; GP96; GRP94; HEL-S-125m; HEL35; hsp108; HSP90B1; HSPC4; TA-3; Targ2; Tra-1; TRA1

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UniProt ID: (Bovine) Q95M18, (Chicken) P08110, (Human) P14625, (Mouse) P08113, (Rat) Q66HD0

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Entrez Gene ID: (Bovine) 282646, (Chicken) 374163, (Human) 7184, (Chinese hamster) 100773331, (Mouse) 22027, (Rat) 362862

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Function(s)

RNA binding ATP binding protein phosphatase binding low-density lipoprotein particle receptor binding unfolded protein binding calcium ion binding protein binding virion binding nucleotide binding Hsp90 family chaperone

Process(es)

response to hypoxia ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol actin rod assembly negative regulation of apoptotic process regulation of phosphoprotein phosphatase activity cellular response to ATP protein folding intracellular sequestering of iron ion toll-like receptor signaling pathway receptor-mediated endocytosis protein transport protein folding in endoplasmic reticulum response to endoplasmic reticulum stress ATF6-mediated unfolded protein response sequestering of calcium ion response to stress

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GRP94 Monoclonal Antibody (9G10)

GRP94 Antibody (MA3-016) in ICC/IF

Product Details

MA3-016

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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