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GRP94 Antibody (MA5-45096) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with GRP94 monoclonal antibody (Product # MA5-45096) at 1:100 for 12 hours at 4°C, followed by R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 antibody. (C) Composit... View More
Product Details
MA5-45096
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Horse,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Sheep,
Pig,
Rabbit,
Rat,
Xenopus
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
9G10
Immunogen
Purified Grp94 isolated from chicken oviducts
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.2, with 2mM EDTA
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931550
Product Specific Information
0.5 µg/mL of MA5-45096 was sufficient for detection of Grp94 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.|Detects approximately 98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.
Target Information
GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 108K heat shock protein; 108K HSP; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; 98 kDa protein kinase; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endoplasmin-like protein; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; ERp99; glucose-regulated protein; glucose-regulated protein GRP94; gp96 homolog; GRP-94; GRP94; Heat shock 108 kDa protein; heat shock protein 108; heat shock protein 108.; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90, beta (Grp94), member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; heat shock protein 90kDa beta, member 1; Heat shock protein family C member 4; heat shock protein gp96; HSP 108; hsp108; HSP90 beta; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Polymorphic tumor rejection antigen 1; PPK 98; stress-inducible tumor rejection antigen gp96; Transferrin-binding protein; transforming growth factor alpha regulated gene 2; tumor rejection antigen; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96
Gene Aliases: ECGP; endoplasmin; ERp99; GP96; GRP94; HEL-S-125m; HEL35; hsp108; HSP90B1; HSPC4; ppk98; TA-3; Targ2; Tra-1; TRA1; XELAEV_18017384mg
UniProt ID: (Bovine) Q95M18, (Chicken) P08110, (Pig) Q29092, (Human) P14625, (Dog) P41148, (Rat) Q66HD0, (Mouse) P08113
response to hypoxia
ER-associated ubiquitin-dependent protein catabolic process
retrograde protein transport, ER to cytosol
actin rod assembly
negative regulation of apoptotic process
regulation of phosphoprotein phosphatase activity
cellular response to ATP
protein folding
intracellular sequestering of iron ion
toll-like receptor signaling pathway
receptor-mediated endocytosis
protein transport
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
ATF6-mediated unfolded protein response
sequestering of calcium ion
response to stress
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