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GSDMD Antibody (MA5-50596) in WB
Western blot analysis of GSDMD in different various lysates. Lane 1: PC-3 cell lysate; Lane 2: SiHa cell lysate; Lane 3: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa. Observed band size: 53 kDa. Exposure time: 2 minutes; 10% SDS-PAGE gel. Primary antibody GSDMD monoclonal antibody (Product # MA5-50596) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody with a dilution of 1:100,000 was used for 1 hour at room temperature. Proteins wer... View More
Product Details
MA5-50596
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
A8D8
Immunogen
Recombinant protein within human GSDMD aa 101-300/484.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3091330
Product Specific Information
Positive control: PC-3 cell lysate, SiHa cell lysate, THP-1 cell lysate.
Predicted band size: 53 kDa
Subcellular Location: Cytosol, Inflammasome; Secreted, Cell membrane.
Target Information
GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D
Gene Aliases: DF5L; DFNA5L; FKSG10; GSDMD; GSDMDC1
UniProt ID: (Human) P57764
Entrez Gene ID: (Human) 79792
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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