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GSDMD Antibody (MA5-50686) in WB
Western blot analysis of GSDMD in different various lysates. Lane 1: PC-12 cell lysate; Lane 2: PMVEC cell lysate; Lane 3: RH-35 cell lysate; Lane 4: L6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa. Observed band size: 53/35 kDa. Exposure time: 2 minutes; 10% SDS-PAGE gel. Primary antibody GSDMD recombinant monoclonal antibody (Product # MA5-50686) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:300,000 was used fo... View More
Product Details
MA5-50686
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PD00-18
Immunogen
Recombinant protein within Gasdermin D full length protein aa 1-484/484.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3093926
Product Specific Information
Positive control: PC-3 cell lysate, NIH/3T3 cell lysate, THP-1 cell lysate, THP-1 cell lysate treated with PMA for 17.5 hours and then treated with LPS for 6 hours, PC-12 cell lysate, PMVEC cell lysate, RH-35 cell lysate, L6 cell lysate.
Predicted band size: 53/30 kDa
Subcellular Location: Cell membrane, Secreted.
Target Information
GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D
Gene Aliases: 1810036L03Rik; AW558049; DF5L; DFNA5L; FKSG10; GSDMD; GSDMDC1; M2-4
UniProt ID: (Human) P57764, (Mouse) Q9D8T2
Entrez Gene ID: (Human) 79792, (Rat) 315084, (Mouse) 69146
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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