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GSK-3alpha Antibody (500-2914) in WB
Western Blot: The 3T3 cell lysate was resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with Rabbit anti GSK-3 alpha (Cat#500-2914) antibody at 1:500 (lane 1). Lane 2 was pre-incubated with immunizing peptide. Observed a major immunoreactive band at molecular weight ~52 kDa.
Product Details
500-2914
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to N-terminus of human GSK3-alpha protein
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with proprietary stabilizer
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Positive control: 3T3 cell lysate
Cellular location: Cytoplasmic.
Target Information
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638). Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686D0638; FA; Factor A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; gsk-3-alpha; OTTHUMP00000198368; Serine/threonine-protein kinase GSK3A
Gene Aliases: 2700086H06Rik; GSK3A
UniProt ID: (Human) P49840, (Rat) P18265, (Mouse) Q2NL51
Entrez Gene ID: (Human) 2931, (Rat) 50686, (Mouse) 606496
protein serine/threonine kinase activity
protein binding
ATP binding
protein kinase A catalytic subunit binding
tau-protein kinase activity
protein kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
regulation of systemic arterial blood pressure
cardiac left ventricle morphogenesis
glycogen metabolic process
protein phosphorylation
dopamine receptor signaling pathway
nervous system development
insulin receptor signaling pathway
negative regulation of UDP-glucose catabolic process
Wnt signaling pathway
positive regulation of cAMP biosynthetic process
negative regulation of TOR signaling
cellular response to insulin stimulus
cellular response to interleukin-3
IRE1-mediated unfolded protein response
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of glycogen biosynthetic process
positive regulation of protein catabolic process
positive regulation of heart contraction
negative regulation of glucose import
negative regulation of insulin receptor signaling pathway
negative regulation of cell growth involved in cardiac muscle cell development
positive regulation of adrenergic receptor signaling pathway
negative regulation of canonical Wnt signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
chemical synaptic transmission, postsynaptic
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
negative regulation of glycogen synthase activity, transferring glucose-1-phosphate
negative regulation of type B pancreatic cell development
negative regulation of glycogen (starch) synthase activity
positive regulation of glycogen (starch) synthase activity
regulation of gene expression by genetic imprinting
signal transduction
positive regulation of peptidyl-threonine phosphorylation
cell migration
positive regulation of peptidyl-serine phosphorylation
hypermethylation of CpG island
positive regulation of transcription from RNA polymerase II promoter
negative regulation of transferase activity
cellular response to lithium ion
cellular response to organic cyclic compound
carbohydrate metabolic process
negative regulation of signal transduction
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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