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GSK3B Antibody (MA1-7621) in WB
Knockout of GSK3B was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR933148_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of GSK3B was performed by loading 60 µg of HeLa wild type (Lane 1), HeLa Cas9 (Lane 2), HeLa GSK3B KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by i... View More
Product Details
MA1-7621
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat,
Xenopus,
Human
Published species
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
21A
Immunogen
Purified recombinant Xenopus laevis protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.2
Contains
0.01% sodium azide
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Dry ice
RRID
AB_1074718
Product Specific Information
MA1-7621 detects GSK3 Beta from human, rat, and Xenopus laevis samples.
MA1-7621 has been successfully used in ELISA, Western blot, Flow cytometry, Immunoprecipitation and Immunohistochemistry procedures.
The MA1-7621 immunogen is a purified recombinant Xenopus laevis protein.
Store at 4ºC short term. For extended storage, keep at -80ºC.
Target Information
GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 2.7.11.26; FA; Factor A; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; GSK3beta isoform; intracellular kinase; Serine/threonine-protein kinase GSK3B; Xgsk-3 protein
Gene Aliases: gsk-3; gsk3; gsk3-beta; GSK3B; gsk3beta; XELAEV_180112401mg; Xgsk-3
UniProt ID: (Human) P49841, (Rat) P18266, (Xenopus) Q91757
Entrez Gene ID: (Human) 2932, (Rat) 84027, (Xenopus) 399097
RNA polymerase II transcription factor binding
p53 binding
protein kinase activity
protein serine/threonine kinase activity
integrin binding
protein binding
ATP binding
beta-catenin binding
kinase activity
protein kinase binding
ubiquitin protein ligase binding
protein kinase A catalytic subunit binding
ionotropic glutamate receptor binding
tau protein binding
tau-protein kinase activity
NF-kappaB binding
transcription factor binding
non-receptor serine/threonine protein kinase
re-entry into mitotic cell cycle
epithelial to mesenchymal transition
positive regulation of cell-matrix adhesion
glycogen metabolic process
regulation of gene expression by genetic imprinting
protein phosphorylation
protein export from nucleus
ER overload response
dopamine receptor signaling pathway
axonogenesis
myoblast fusion
aging
circadian rhythm
organ morphogenesis
negative regulation of cardiac muscle hypertrophy
positive regulation of peptidyl-threonine phosphorylation
positive regulation of mitochondrial membrane potential
negative regulation of neuron maturation
response to activity
Wnt signaling pathway
cell migration
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
hippocampus development
establishment of cell polarity
negative regulation of protein complex assembly
positive regulation of protein complex assembly
negative regulation of protein binding
positive regulation of protein binding
response to estradiol
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
response to insulin
regulation of microtubule-based process
positive regulation of peptidyl-serine phosphorylation
negative regulation of smooth muscle cell apoptotic process
protein localization to microtubule
intracellular signal transduction
cellular response to hepatocyte growth factor stimulus
cellular response to interleukin-3
cellular response to erythropoietin
response to drug
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of MAP kinase activity
positive regulation of neuron apoptotic process
positive regulation of GTPase activity
hypermethylation of CpG island
canonical Wnt signaling pathway involved in positive regulation of apoptotic process
fat cell differentiation
negative regulation of glycogen biosynthetic process
positive regulation of protein catabolic process
positive regulation of axon extension
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
positive regulation of protein export from nucleus
regulation of neuronal synaptic plasticity
positive regulation of smooth muscle cell proliferation
negative regulation of dendrite morphogenesis
negative regulation of nitric-oxide synthase activity
negative regulation of NFAT protein import into nucleus
canonical Wnt signaling pathway
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
superior temporal gyrus development
cellular response to mechanical stimulus
cellular response to iron(II) ion
cellular response to lithium ion
response to epinephrine
negative regulation of canonical Wnt signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
chemical synaptic transmission, postsynaptic
regulation of cellular response to heat
negative regulation of protein localization to nucleus
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
positive regulation of neuron death
response to L-glutamate
cellular response to dopamine
negative regulation of dopaminergic neuron differentiation
beta-catenin destruction complex assembly
beta-catenin destruction complex disassembly
response to insulin-like growth factor stimulus
response to ultrasound
response to angiotensin
negative regulation of type B pancreatic cell development
positive regulation of excitatory postsynaptic potential
negative regulation of glycogen (starch) synthase activity
positive regulation of DNA biosynthetic process
positive regulation of stem cell differentiation
negative regulation of neuron migration
establishment or maintenance of cell polarity
response to lithium ion
regulation of neuron projection development
negative regulation of neuron projection development
myotube differentiation
phosphorylation
positive regulation of apoptotic process
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