GenScript

GST Monoclonal Antibody (2F10B9), HRP, THE™

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GST Antibody (A00866-100) in WB

Western blot analysis of GST-tagged fusion protein using THE™ GST Antibody [HRP], mAb, Mouse (GenScript, A00866, 0.1 µg/mL). The signal was developed with LumiSensor TM HRP Substrate Kit (GenScript, L00221). Predicted Size: 26 kD. Observed Size: 26 kD.

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Product Details

A00866-100

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

ELISA (ELISA)

0.05-0.2 µg/mL

Miscellaneous PubMed (Misc)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2F10B9

Immunogen

Recombinant GST protein (26 kD)

Conjugate

HRP HRP HRP

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.01% thimerosal

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

GenScript THE™ GST Antibody [HRP], mAb, Mouse is HRP conjugated THE™ Anti-GST Monoclonal Antibody (Mouse) (A00865) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of GST in fusion proteins.

Target Information

GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TOP1α regulates FLOWERING LOCUS C expression by coupling histone modification and transcription machinery.

Development (Cambridge, England)

Zhong P,Li J,Luo L,Zhao Z,Tian Z

The key steps of transcription are coupled with the opening of the DNA helical structure and establishment of active chromatin to facilitate the movement of the transcription machinery. Type I topoisomerases cleave one DNA strand and relax the supercoiled structure of transcribed templates. How topoisomerase-mediated DNA topological changes promote transcription and establish a permissive histone modification for transcription elongation is largely unknown. Here we show that TOPOISOMERASE 1&alph

Fri Feb 15 00:00:00 EST 2019

Powerdress as the novel regulator enhances Arabidopsis seeds germination tolerance to high temperature stress by histone modification of SOM locus.

Plant science : an international journal of experimental plant biology

Yang W,Chen Z,Huang Y,Chang G,Li P,Wei J,Yuan X,Huang J,Hu X

Seeds germination or dormancy is strictly controlled by endogenous phytohormone signal and environment cues. High temperature (HT) suppresses seeds germination or triggers seeds dormancy but underlying mechanism by which HT mediates seeds germination thermoinhibition needs more investigating. SOM is reported as the critical factor negatively controls light-irradiation seeds germination by altering Abscisic acid (ABA) and gibberellin acid (GA) biosynthesis. Here we found that HT accelerates SOM e

Mon Jul 01 00:00:00 EDT 2019

PRC2 recruitment and H3K27me3 deposition at FLC require FCA binding of COOLAIR.

Science advances

Tian Y,Zheng H,Zhang F,Wang S,Ji X,Xu C,He Y,Ding Y

The cold-induced antisense transcript COOLAIR represses FLOWERING LOCUS C (FLC) transcription with increased H3K27me3 and decreased H3K36me3 levels in response to cold temperatures. However, the molecular connection between COOLAIR and histone modification factors in the absence of cold treatment remains unclear. We report that the RNA binding protein FCA interacts with the PRC2 subunit CURLY LEAF (CLF) and binds nascent COOLAIR transcripts to allow deposition of H3K27me3 at FLC. Loss of COOLAIR

Mon Apr 01 00:00:00 EDT 2019

MLK1 and MLK2 Coordinate RGA and CCA1 Activity to Regulate Hypocotyl Elongation in Arabidopsis thaliana.

The Plant cell

Zheng H,Zhang F,Wang S,Su Y,Ji X,Jiang P,Chen R,Hou S,Ding Y

Gibberellins (GAs) modulate diverse developmental processes throughout the plant life cycle. However the interaction between GAs and the circadian rhythm remains unclear. Here we report that MUT9p-LIKE KINASE1 (MLK1) and MLK2 mediate the interaction between GAs and the circadian clock to regulate hypocotyl elongation in DELLA proteins function as master growth repressors that integrate phytohormone signaling and environmental pathways in plant development. MLK1 and MLK2 interact with the DELLA

Mon Jan 01 00:00:00 EST 2018

The COMPASS-Like Complex Promotes Flowering and Panicle Branching in Rice.

Plant physiology

Jiang P,Wang S,Jiang H,Cheng B,Wu K,Ding Y

Flowering time (heading date) and panicle branch number are important agronomic traits that determine yield in rice (Oryza sativa). The activation of flowering requires histone methylation, but the roles of trimethylation of Lys 4 of histone 3 (H3K4me3) in modulating heading date and panicle development are unclear. Here, we showed that the COMPASS-like complex promotes flowering and panicle branching. The rice (Oryza sativa) WD40 protein OsWDR5a interacts with the TRITHORAX-like protein OsTrx1/

Sun Apr 01 00:00:00 EDT 2018

Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins.

The EMBO journal

Stolz A,Putyrski M,Kutle I,Huber J,Wang C,Major V,Sidhu SS,Youle RJ,Rogov VV,Dötsch V,Ernst A,Dikic I

Autophagy is a cellular surveillance pathway that balances metabolic and energy resources and transports specific cargos including damaged mitochondria other broken organelles or pathogens for degradation to the lysosome. Central components of autophagosomal biogenesis are six members of the LC3 and GABARAP family of ubiquitin-like proteins (mATG8s). We used phage display to isolate peptides that possess LIR (LC3-interacting region) properties and are selective for individual mATG8 isoforms. Se

Wed Feb 15 00:00:00 EST 2017

Phosphorylation of Histone H2A at Serine 95: A Plant-Specific Mark Involved in Flowering Time Regulation and H2A.Z Deposition.

The Plant cell

Su Y,Wang S,Zhang F,Zheng H,Liu Y,Huang T,Ding Y

Phosphorylation of histone H3 affects transcription chromatin condensation and chromosome segregation. However the role of phosphorylation of histone H2A remains unclear. Here we found that MUT9P-LIKE-KINASE (MLK4) phosphorylates histone H2A on serine 95 a plant-specific modification in the histone core domain. Mutations in caused late flowering under long-day conditions but no notable phenotype under short days. MLK4 interacts with CIRCADIAN CLOCK ASSOCIATED1 (CCA1) which allows MLK4 to bind

Fri Sep 01 00:00:00 EDT 2017

A quartet of PIF bHLH factors provides a transcriptionally centered signaling hub that regulates seedling morphogenesis through differential expression-patterning of shared target genes in Arabidopsis.

PLoS genetics

Zhang Y,Mayba O,Pfeiffer A,Shi H,Tepperman JM,Speed TP,Quail PH

Dark-grown seedlings exhibit skotomorphogenic development. Genetic and molecular evidence indicates that a quartet of Arabidopsis Phytochrome (phy)-Interacting bHLH Factors (PIF1, 3, 4, and 5) are critically necessary to maintaining this developmental state and that light activation of phy induces a switch to photomorphogenic development by inducing rapid degradation of the PIFs. Here, using integrated ChIP-seq and RNA-seq analyses, we have identified genes that are direct targets of PIF3 transc

Thu May 30 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag

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