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GST Antibody (A00867-200) in WB
Western blot analysis of GST-tagged fusion protein using THE™ GST Antibody [Biotin], mAb, Mouse (GenScript, A00867, 1 µg/mL). The signal was developed with Streptaidin-HRP (GenScript, M00091, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221). Predicted Size: 31 kD. Observed Size: 31 kD.
Product Details
A00867-200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2F10B9
Immunogen
Recombinant GST protein (26 kD)
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
GenScript THE™ GST Antibody [Biotin], mAb, Mouse is the biotin conjugated THE™ Anti-GST Monoclonal Antibody (Mouse) (A00865) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of GST in fusion proteins.
Target Information
GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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