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GST Antibody (740007M) in ICC/IF
Immunofluorescent analysis of GST Tag was performed using 70% confluent mCherry-GST transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with GST Tag Recombinant Mouse Monoclonal Antibody (GST 3-4C) (Product # 740007M, 1:200) at 4°C overnight and then labeled with Goat anti-Mouse IgG Fc Cross-Adsorbed Secondary Antibody, APC (Product # 31981, 1:125) for 1 hour at room temper... View More
Product Details
740007M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG2b, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
GST 3-4C
Immunogen
GST protein from Schistosoma japonicum expressed from the pGEX vector.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074177
Product Specific Information
GST Tag recombinant mouse monoclonal antibody specifically recognizes GST Tag at both C and N-terminus of proteins.
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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