Invitrogen

GST Tag Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

9 Published Figures

21 References

View all (97) GST antibodies

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GST Tag Antibody (71-7500) in WB

Western blot was performed using GST Tag Polyclonal Antibody (Product # 71-7500) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), H3-GST, 50 µg (Lane 3), H3-GST, 25 µg (Lane 4), H3-GST, 12.5 µg (Lane 5), H3-GST, 6.25 µg (Lane 6), GST-p65, 50 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) b... View More

GST Tag Antibody in Immunocytochemistry (ICC/IF)

GST Tag Antibody in Immunoprecipitation (IP)

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Product Details

71-7500

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

ELISA (ELISA)

0.1-1.0 µg/mL

Immunoprecipitation (IP)

View 3 publications 3 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified glutathione S-transferase (GST) protein from Schistosoma japonicum expressed from a pGEX vector

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533994

Target Information

GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Broad-Based Characterization of a Cell-Penetrating, Single Domain Camelid Bi-Specific Antibody Monomer That Targets STAT3 and KRAS Dependent Cancers.

International journal of molecular sciences

Singh S,Murillo G,Richner J,Singh SP,Berleth E,Kumar V,Mehta R,Ramiya V,Parihar AS

71-7500 was used in Immunocytochemistry-immunofluorescence to report a first-in-class molecule, a novel, single domain camelid VHH antibody (15 kDa), SBT-100, that binds to both STAT3 and KRAS and can penetrate the tumor cell membrane, and significantly inhibit cancer cell growth.

Fri Jul 08 00:00:00 EDT 2022

Mechanical control of nuclear import by Importin-7 is regulated by its dominant cargo YAP.

Nature communications

García-García M,Sánchez-Perales S,Jarabo P,Calvo E,Huyton T,Fu L,Ng SC,Sotodosos-Alonso L,Vázquez J,Casas-Tintó S,Görlich D,Echarri A,Del Pozo MA

71-7500 was used in Western Blot to show that the nuclear import process is an additional regulatory layer indirectly regulated by mechanical cues, which activate a preferential Imp7 cargo, YAP, which competes out other cargoes, resulting in signaling crosstalk.

Fri Mar 04 00:00:00 EST 2022

Structural basis of the human Scribble-Vangl2 association in health and disease.

The Biochemical journal

How JY,Stephens RK,Lim KYB,Humbert PO,Kvansakul M

71-7500 was used in Western Blot to study the oncogenic and tumour suppressor functions of the Scribble-Vangl2 complex.

Fri Apr 16 00:00:00 EDT 2021

WW, PH and C-Terminal Domains Cooperate to Direct the Subcellular Localizations of PLEKHA5, PLEKHA6 and PLEKHA7.

Frontiers in cell and developmental biology

Sluysmans S,Méan I,Jond L,Citi S

71-7500 was used in Western Blot to study the role of WW-PLEKHAs structural domains in their subcellular localization.

Wed Sep 29 00:00:00 EDT 2021

Toll-like receptor 2 stimulation augments esophageal barrier integrity.

Allergy

Ruffner MA,Song L,Maurer K,Shi L,Carroll MC,Wang JX,Muir AB,Spergel JM,Sullivan KE

Published figure using GST Tag polyclonal antibody (Product # 71-7500) in ChIP assay

Sun Dec 01 00:00:00 EST 2019

The major leaf ferredoxin Fd2 regulates plant innate immunity in Arabidopsis.

Molecular plant pathology

Wang M,Rui L,Yan H,Shi H,Zhao W,Lin JE,Zhang K,Blakeslee JJ,Mackey D,Tang D,Wei Z,Wang GL

Published figure using GST Tag polyclonal antibody (Product # 71-7500) in Immunoprecipitation

Fri Jun 01 00:00:00 EDT 2018

The major leaf ferredoxin Fd2 regulates plant innate immunity in Arabidopsis.

Molecular plant pathology

Wang M,Rui L,Yan H,Shi H,Zhao W,Lin JE,Zhang K,Blakeslee JJ,Mackey D,Tang D,Wei Z,Wang GL

Published figure using GST Tag polyclonal antibody (Product # 71-7500) in Immunoprecipitation

Fri Jun 01 00:00:00 EDT 2018

SUMOs Mediate the Nuclear Transfer of p38 and p-p38 during Helicobacter Pylori Infection.

International journal of molecular sciences

Wang PY,Hsu PI,Wu DC,Chen TC,Jarman AP,Powell LM,Chen A

Published figure using GST Tag polyclonal antibody (Product # 71-7500) in Western Blot

Wed Aug 22 00:00:00 EDT 2018

SCIMP is a transmembrane non-TIR TLR adaptor that promotes proinflammatory cytokine production from macrophages.

Nature communications

Luo L,Bokil NJ,Wall AA,Kapetanovic R,Lansdaal NM,Marceline F,Burgess BJ,Tong SJ,Guo Z,Alexandrov K,Ross IL,Hibbs ML,Stow JL,Sweet MJ

71-7500 was used in western blot to elucidate the role of SCIMP in Toll-like receptor signaling

Wed Jan 18 00:00:00 EST 2017

Small GTPase Rab8a-recruited Phosphatidylinositol 3-Kinase γ Regulates Signaling and Cytokine Outputs from Endosomal Toll-like Receptors.

The Journal of biological chemistry

Wall AA,Luo L,Hung Y,Tong SJ,Condon ND,Blumenthal A,Sweet MJ,Stow JL

71-7500 was used in Western Blotting to study the array of immune receptors controlled by Rab8a-PI3Kγ and further define the Rab-mediated membrane domains required for signalling.

Fri Mar 17 00:00:00 EDT 2017

C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles.

Cell

Lee KH,Zhang P,Kim HJ,Mitrea DM,Sarkar M,Freibaum BD,Cika J,Coughlin M,Messing J,Molliex A,Maxwell BA,Kim NC,Temirov J,Moore J,Kolaitis RM,Shaw TI,Bai B,Peng J,Kriwacki RW,Taylor JP

71-7500 was used in western blot to report the interactome of all dipeptide repeat proteins

Thu Oct 20 00:00:00 EDT 2016

PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3.

eLife

Chung HR,Xu C,Fuchs A,Mund A,Lange M,Staege H,Schubert T,Bian C,Dunkel I,Eberharter A,Regnard C,Klinker H,Meierhofer D,Cozzuto L,Winterpacht A,Di Croce L,Min J,Will H,Kinkley S

71-7500 was used in immunoprecipitation to assess the molecular reader and transcriptional co-regulator of H3k4me2/3 called PHF13

Wed May 25 00:00:00 EDT 2016

PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3.

eLife

Chung HR,Xu C,Fuchs A,Mund A,Lange M,Staege H,Schubert T,Bian C,Dunkel I,Eberharter A,Regnard C,Klinker H,Meierhofer D,Cozzuto L,Winterpacht A,Di Croce L,Min J,Will H,Kinkley S

71-7500 was used in immunoprecipitation to assess the molecular reader and transcriptional co-regulator of H3k4me2/3 called PHF13

Wed May 25 00:00:00 EDT 2016

Rab8a interacts directly with PI3Kγ to modulate TLR4-driven PI3K and mTOR signalling.

Nature communications

Luo L,Wall AA,Yeo JC,Condon ND,Norwood SJ,Schoenwaelder S,Chen KW,Jackson S,Jenkins BJ,Hartland EL,Schroder K,Collins BM,Sweet MJ,Stow JL

71-7500 was used in western blot to examiine how Rab8a and PI3Kgamma regulate TLR4 signaling.

Tue Jul 15 00:00:00 EDT 2014

RECQL4 is essential for the transport of p53 to mitochondria in normal human cells in the absence of exogenous stress.

Journal of cell science

De S,Kumari J,Mudgal R,Modi P,Gupta S,Futami K,Goto H,Lindor NM,Furuichi Y,Mohanty D,Sengupta S

71-7500 was used in immunoprecipitation to elucidate the mechanism by which p53 is regulated by RECQL4.

Tue May 15 00:00:00 EDT 2012

HIV-1 activates Cdc42 and induces membrane extensions in immature dendritic cells to facilitate cell-to-cell virus propagation.

Blood

Nikolic DS,Lehmann M,Felts R,Garcia E,Blanchet FP,Subramaniam S,Piguet V

Thu Nov 03 00:00:00 EDT 2011

Interactome mapping suggests new mechanistic details underlying Alzheimer's disease.

Genome research

Soler-López M,Zanzoni A,Lluís R,Stelzl U,Aloy P

71-7500 was used in western blot to report and assess the most complete interactome of genes and proteins associated with Alzheimer's disease.

Tue Mar 01 00:00:00 EST 2011

Active nuclear import and export pathways regulate E2F-5 subcellular localization.

The Journal of biological chemistry

Apostolova MD,Ivanova IA,Dagnino C,D'Souza SJ,Dagnino L

Published figure using GST Tag polyclonal antibody (Product # 71-7500) in Immunocytochemistry

Fri Sep 13 00:00:00 EDT 2002

Active nuclear import and export pathways regulate E2F-5 subcellular localization.

The Journal of biological chemistry

Apostolova MD,Ivanova IA,Dagnino C,D'Souza SJ,Dagnino L

Published figure using GST Tag polyclonal antibody (Product # 71-7500) in Immunocytochemistry

Fri Sep 13 00:00:00 EDT 2002

SH2-B family members differentially regulate JAK family tyrosine kinases.

The Journal of biological chemistry

O'Brien KB,O'Shea JJ,Carter-Su C

Fri Mar 08 00:00:00 EST 2002

The polo-box-dependent induction of ectopic septal structures by a mammalian polo kinase, plk, in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America

Lee KS,Song S,Erikson RL

Tue Dec 07 00:00:00 EST 1999

Bioinformatics

Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GST Tag Polyclonal Antibody

GST Tag Antibody (71-7500) in WB

Product Details

71-7500

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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