Product References
Experimental Investigations on the Structure of Yeast Mitochondrial Pyruvate Carriers.
Membranes
Li L,Wen M,Run C,Wu B,OuYang B
A-5800 was used in Mass Spectrometry to express and purify large amounts of yeast MPC1 and MPC2 for structural characterization of MPC complex.
Thu Sep 22 00:00:00 EDT 2022
Experimental Investigations on the Structure of Yeast Mitochondrial Pyruvate Carriers.
Membranes
Li L,Wen M,Run C,Wu B,OuYang B
A-5800 was used in Mass Spectrometry to express and purify large amounts of yeast MPC1 and MPC2 for structural characterization of MPC complex.
Thu Sep 22 00:00:00 EDT 2022
Bro1 binds the Vps20 subunit of ESCRT-III and promotes ESCRT-III regulation by Doa4.
Traffic (Copenhagen, Denmark)
Buysse D,West M,Leih M,Odorizzi G
A-5800 was used in Western Blotting to show that Bro1 binds directly to Vps20, suggesting that Bro1 has a central role in relieving the antagonistic relationship that Vps20 has toward Doa4.
Tue Feb 01 00:00:00 EST 2022
Activity of the nonreceptor tyrosine kinase Ack1 is regulated by tyrosine phosphorylation of its Mig6 homology region.
FEBS letters
Kan Y,Miller WT
A-5800 was used in Western Blotting to test whether intramolecular interactions between the Ack1 MHR and kinase domain (KD) are regulated by phosphorylation.
Tue Nov 01 00:00:00 EDT 2022
Recreational water exposure and waterborne infections in a prospective salivary antibody study at a Lake Michigan beach.
Scientific reports
Egorov AI,Converse R,Griffin SM,Bonasso R,Wickersham L,Klein E,Kobylanski J,Ritter R,Styles JN,Ward H,Sams E,Hudgens E,Dufour A,Wade TJ
A-5800 was used in Luminex to contribute further evidence that recreational water exposure is associated with symptomatic and asymptomatic waterborne infections, and that salivary antibody assays can be used in epidemiological surveys of norovirus and Cryptosporidium infections.
Fri Oct 15 00:00:00 EDT 2021
CAP1 binds and activates adenylyl cyclase in mammalian cells.
Proceedings of the National Academy of Sciences of the United States of America
Zhang X,Pizzoni A,Hong K,Naim N,Qi C,Korkhov V,Altschuler DL
A-5800 was used in ChIP assay to indicate that CAP1-cyclase-Rap1 represents a regulatory unit in cAMP dynamics and biology.
Tue Jun 15 00:00:00 EDT 2021
Heterogeneous Nuclear Ribonucleoprotein A1 (hnRNP A1) and hnRNP A2 Inhibit Splicing to Human Papillomavirus 16 Splice Site SA409 through a UAG-Containing Sequence in the E7 Coding Region.
Journal of virology
Zheng Y,Jönsson J,Hao C,Shoja Chaghervand S,Cui X,Kajitani N,Gong L,Wu C,Schwartz S
A-5800 was used in Western Blotting to determine how splicing between SD226 and SA409 is regulated.
Tue Sep 29 00:00:00 EDT 2020
Heterogeneous Nuclear Ribonucleoprotein A1 (hnRNP A1) and hnRNP A2 Inhibit Splicing to Human Papillomavirus 16 Splice Site SA409 through a UAG-Containing Sequence in the E7 Coding Region.
Journal of virology
Zheng Y,Jönsson J,Hao C,Shoja Chaghervand S,Cui X,Kajitani N,Gong L,Wu C,Schwartz S
A-5800 was used in Western Blotting to determine how splicing between SD226 and SA409 is regulated.
Tue Sep 29 00:00:00 EDT 2020
The ubiquitin hydrolase Doa4 directly binds Snf7 to inhibit recruitment of ESCRT-III remodeling factors in S. cerevisiae.
Journal of cell science
Buysse D,Pfitzner AK,West M,Roux A,Odorizzi G
A-5800 was used in Western Blotting to report that the non-catalytic N-terminus of Doa4 binds Snf7, the predominant ESCRT-III subunit.
Tue Apr 28 00:00:00 EDT 2020
Systematic identification of CDC34 that functions to stabilize EGFR and promote lung carcinogenesis.
EBioMedicine
Zhao XC,Wang GZ,Wen ZS,Zhou YC,Hu Q,Zhang B,Qu LW,Gao SH,Liu J,Ma L,Zhang YF,Zhang C,Yu H,Zhang DL,Wang M,Wang CL,Huang YC,Liu ZH,Zhao Y,Chen L,Zhou GB
Published figure using GST Tag polyclonal antibody (Product # A-5800) in Western Blot
Sun Mar 01 00:00:00 EST 2020
Disease-Causing Mutations in SF3B1 Alter Splicing by Disrupting Interaction with SUGP1.
Molecular cell
Zhang J,Ali AM,Lieu YK,Liu Z,Gao J,Rabadan R,Raza A,Mukherjee S,Manley JL
A-5800 was used in Western Blotting to analyse RNA sequencing data from myelodysplastic syndrome patients and confirm if SF3B1 mutants use aberrant 3' splice sites.
Thu Oct 03 00:00:00 EDT 2019
Sensitive detection of type G botulinum neurotoxin through Endopep-MS peptide substrate optimization.
Analytical and bioanalytical chemistry
Wang D,Baudys J,Hoyt K,Barr JR,Kalb SR
A-5800 was used in Mass cytometry to optimise synthetic peptide substrates for use in a mass spectrometry-based Endopep-MS assay that is able to rapidly detect and differentiate botulinum neurotoxin serotypes A-G by immunoaffinity capture of toxins and detection of unique cleavage products of peptide substrates.
Thu Aug 01 00:00:00 EDT 2019
Modeling ErbB2-p130Cas interaction to design new potential anticancer agents.
Scientific reports
Costamagna A,Rossi Sebastiano M,Natalini D,Simoni M,Valabrega G,Defilippi P,Visentin S,Ermondi G,Turco E,Caron G,Cabodi S
A-5800 was used in Western Blotting to identify the p130Cas/ErbB2 complex as a potential breast cancer target revealing new therapeutic perspectives for protein-protein interaction.
Thu Feb 28 00:00:00 EST 2019
Modeling ErbB2-p130Cas interaction to design new potential anticancer agents.
Scientific reports
Costamagna A,Rossi Sebastiano M,Natalini D,Simoni M,Valabrega G,Defilippi P,Visentin S,Ermondi G,Turco E,Caron G,Cabodi S
A-5800 was used in Western Blotting to identify the p130Cas/ErbB2 complex as a potential breast cancer target revealing new therapeutic perspectives for protein-protein interaction.
Thu Feb 28 00:00:00 EST 2019
Explorative Study on Isolation and Characterization of a Microviridae G4 Bacteriophage, EMCL318, against Multi-Drug-resistant Escherichia coli 15-318.
Antibiotics (Basel, Switzerland)
Ghosh S,Persad E,Shiue TY,Lam C,Islam A,Mascibroda LG,Sherman MB,Smith T,Cheeptham N
A-5800 was used in Mass Spectrometry to reveal the phage as alternative potentials to fight against emerging and existing multi-drug-resistant infectious diseases such as E. coli 15-318.
Fri Oct 26 00:00:00 EDT 2018
Characterization of Plasmodium vivax Proteins in Plasma-Derived Exosomes From Malaria-Infected Liver-Chimeric Humanized Mice.
Frontiers in microbiology
Gualdrón-López M,Flannery EL,Kangwanrangsan N,Chuenchob V,Fernandez-Orth D,Segui-Barber J,Royo F,Falcón-Pérez JM,Fernandez-Becerra C,Lacerda MVG,Kappe SHI,Sattabongkot J,Gonzalez JR,Mikolajczak SA,Del Portillo HA
Published figure using GST Tag polyclonal antibody (Product # A-5800) in Western Blot
Thu Oct 01 00:00:00 EDT 2020
Tyrosine phosphorylation switching of a G protein.
The Journal of biological chemistry
Li B,Tunc-Ozdemir M,Urano D,Jia H,Werth EG,Mowrey DD,Hicks LM,Dokholyan NV,Torres MP,Jones AM
A-5800 was used in in vitro experiments to propose that phosphorylation of Tyr166 in AtGPA1 changes the binding pattern with AtRGS1 and thereby attenuates the steady-state rate of the GTPase cycle.
Fri Mar 30 00:00:00 EDT 2018
Tyrosine phosphorylation switching of a G protein.
The Journal of biological chemistry
Li B,Tunc-Ozdemir M,Urano D,Jia H,Werth EG,Mowrey DD,Hicks LM,Dokholyan NV,Torres MP,Jones AM
A-5800 was used in in vitro experiments to propose that phosphorylation of Tyr166 in AtGPA1 changes the binding pattern with AtRGS1 and thereby attenuates the steady-state rate of the GTPase cycle.
Fri Mar 30 00:00:00 EDT 2018
TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network.
Nature communications
Renna L,Stefano G,Slabaugh E,Wormsbaecher C,Sulpizio A,Zienkiewicz K,Brandizzi F
A-5800 was used in Western Blotting to study the mechanisms underlying trans-golgi network biogenesis and function.
Fri Dec 14 00:00:00 EST 2018
E-cadherin cleavage by MT2-MMP regulates apical junctional signaling and epithelial homeostasis in the intestine.
Journal of cell science
Gómez-Escudero J,Moreno V,Martín-Alonso M,Hernández-Riquer MV,Feinberg T,Colmenar Á,Calvo E,Camafeita E,Martínez F,Oudhoff MJ,Weiss SJ,Arroyo AG
A-5800 was used in an ELISA assay to characterise an unexpected role for the membrane-anchored metalloproteinase, MT2-MMP, in regulating epithelial cell quiescence.
Fri Dec 01 00:00:00 EST 2017
GRP78 Participates in PCA3-regulated Prostate Cancer Progression.
Anticancer research
Lin C,Wang J,Wang Y,Zhu P,Liu X,Li N,Liu J,Yu L,Wang W
A-5800 was used in Gel Electrophoresis to confirm that, in prostate cancer (PCa), PCA3 plays a pro-cancer role through promoting cell proliferation, migration and invasion while inhibiting cell apoptosis, suggesting that this process might involve the up-regulation of GRP78.
Tue Aug 01 00:00:00 EDT 2017
Staphylococcus aureus recruits Cdc42GAP through recycling endosomes and the exocyst to invade human endothelial cells.
Journal of cell science
Rauch L,Hennings K,Trasak C,Röder A,Schröder B,Koch-Nolte F,Rivera-Molina F,Toomre D,Aepfelbacher M
A-5800 was used in Immunocytochemistry-immunoflourescence to show that after stimulating actin polymerisation, staphylococci recruit Cdc42GAP which deactivates Cdc42 and terminates actin polymerisation in the phagocytic cups.
Mon Aug 01 00:00:00 EDT 2016
The Clinical Significance of Glycoprotein Phospholipase D Levels in Distinguishing Early Stage Latent Autoimmune Diabetes in Adults and Type 2 Diabetes.
PloS one
Qin W,Liang YZ,Qin BY,Zhang JL,Xia N
A-5800 was used in Western Blotting to implicate glycoprotein phospholipase D as a plasma protein for determining early stage latent autoimmune diabetes in adults and type 2 diabetes.
Mon Jul 24 00:00:00 EDT 2017
The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.
Developmental cell
Liew GM,Ye F,Nager AR,Murphy JP,Lee JS,Aguiar M,Breslow DK,Gygi SP,Nachury MV
A-5800 was used in immunoprecipitation to determine how and where ARL6 is activated in cilia
Mon Nov 10 00:00:00 EST 2014
Identification of new mechanisms of cellular response to chemotherapy by tracking changes in post-translational modifications by ubiquitin and ubiquitin-like proteins.
Journal of proteome research
Bonacci T,Audebert S,Camoin L,Baudelet E,Bidaut G,Garcia M,Witzel II,Perkins ND,Borg JP,Iovanna JL,Soubeyran P
A-5800 was used in western blot to examine the ubiquitin-, Nedd8-, and SUMO1-specific proteomes of a pancreatic cancer cell line treated with gemcitabine.
Fri May 02 00:00:00 EDT 2014
Menin epigenetically represses Hedgehog signaling in MEN1 tumor syndrome.
Cancer research
Gurung B,Feng Z,Iwamoto DV,Thiel A,Jin G,Fan CM,Ng JM,Curran T,Hua X
A-5800 was used in western blot to
Mon Apr 15 00:00:00 EDT 2013
The recruitment of the Saccharomyces cerevisiae Paf1 complex to active genes requires a domain of Rtf1 that directly interacts with the Spt4-Spt5 complex.
Molecular and cellular biology
Mayekar MK,Gardner RG,Arndt KM
Published figure using GST Tag polyclonal antibody (Product # A-5800) in Western Blot
Thu Aug 01 00:00:00 EDT 2013
A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin.
PloS one
Hämälistö S,Pouwels J,de Franceschi N,Saari M,Ivarsson Y,Zimmermann P,Brech A,Stenmark H,Ivaska J
Published figure using GST Tag polyclonal antibody (Product # A-5800) in ELISA
Wed May 14 00:00:00 EDT 2014
Tyr728 in the kinase domain of the murine kinase suppressor of RAS 1 regulates binding and activation of the mitogen-activated protein kinase kinase.
The Journal of biological chemistry
Sibilski C,Mueller T,Kollipara L,Zahedi RP,Rapp UR,Rudel T,Baljuls A
A-5800 was used in immunoprecipitation and western blot to investigate how phosphorylation regulates KSR.
Fri Dec 06 00:00:00 EST 2013
The collagen-binding integrin α2β1 is a novel interaction partner of the Trimeresurus flavoviridis venom protein flavocetin-A.
The Journal of biological chemistry
Arlinghaus FT,Eble JA
A-5800 was used in Inhibition experiments to study the α2Β1 integrin-blocking component from the venom of the habu snake (Trimeresurus flavoviridis).
Fri Jan 11 00:00:00 EST 2013
The collagen-binding integrin α2β1 is a novel interaction partner of the Trimeresurus flavoviridis venom protein flavocetin-A.
The Journal of biological chemistry
Arlinghaus FT,Eble JA
A-5800 was used in Inhibition experiments to study the α2Β1 integrin-blocking component from the venom of the habu snake (Trimeresurus flavoviridis).
Fri Jan 11 00:00:00 EST 2013
The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex.
Genes & development
David CJ,Boyne AR,Millhouse SR,Manley JL
Published figure using GST Tag polyclonal antibody (Product # A-5800) in Immunoprecipitation
Sun May 01 00:00:00 EDT 2011
Arf6 regulates AP-1B-dependent sorting in polarized epithelial cells.
The Journal of cell biology
Shteyn E,Pigati L,Fölsch H
A-5800 was used in Western Blotting to demonstrate that activated ADP-ribosylation factor 6 directs membrane recruitment of AP-1B, thus regulating AP-1B's functions in polarised epithelial cells.
Mon Sep 19 00:00:00 EDT 2011
Arf6 regulates AP-1B-dependent sorting in polarized epithelial cells.
The Journal of cell biology
Shteyn E,Pigati L,Fölsch H
A-5800 was used in Western Blotting to demonstrate that activated ADP-ribosylation factor 6 directs membrane recruitment of AP-1B, thus regulating AP-1B's functions in polarised epithelial cells.
Mon Sep 19 00:00:00 EDT 2011
Ypt31/32 GTPases and their F-Box effector Rcy1 regulate ubiquitination of recycling proteins.
Cellular logistics
Chen SH,Shah AH,Segev N
Published figure using GST Tag polyclonal antibody (Product # A-5800) in Western Blot
Sat Jan 01 00:00:00 EST 2011
Degradation of p21Cip1 through anaphase-promoting complex/cyclosome and its activator Cdc20 (APC/CCdc20) ubiquitin ligase complex-mediated ubiquitylation is inhibited by cyclin-dependent kinase 2 in cardiomyocytes.
The Journal of biological chemistry
Yamada K,Tamamori-Adachi M,Goto I,Iizuka M,Yasukawa T,Aso T,Okazaki T,Kitajima S
A-5800 was used in immunoprecipitation to study the role of ubiquitin-mediated degradation of p2Cip in cell cycle arrest and terminal differentiation.
Fri Dec 23 00:00:00 EST 2011
Degradation of p21Cip1 through anaphase-promoting complex/cyclosome and its activator Cdc20 (APC/CCdc20) ubiquitin ligase complex-mediated ubiquitylation is inhibited by cyclin-dependent kinase 2 in cardiomyocytes.
The Journal of biological chemistry
Yamada K,Tamamori-Adachi M,Goto I,Iizuka M,Yasukawa T,Aso T,Okazaki T,Kitajima S
A-5800 was used in immunoprecipitation to study the role of ubiquitin-mediated degradation of p2Cip in cell cycle arrest and terminal differentiation.
Fri Dec 23 00:00:00 EST 2011
Cep70 and Cep131 contribute to ciliogenesis in zebrafish embryos.
BMC cell biology
Wilkinson CJ,Carl M,Harris WA
A-5800 was used in Western Blotting to understand the contribution of centrosome proteins, Cep70 and Cep131, to cilliogenesis in the zebrafish embryo.
Mon Mar 02 00:00:00 EST 2009
LIS1 and NDEL1 coordinate the plus-end-directed transport of cytoplasmic dynein.
The EMBO journal
Yamada M,Toba S,Yoshida Y,Haratani K,Mori D,Yano Y,Mimori-Kiyosue Y,Nakamura T,Itoh K,Fushiki S,Setou M,Wynshaw-Boris A,Torisawa T,Toyoshima YY,Hirotsune S
A-5800 was used in Western Blotting to elucidate a novel model of the regulation of cytoplasmic dynein by LIS1.
Wed Oct 08 00:00:00 EDT 2008
Functional dissection of the NuA4 histone acetyltransferase reveals its role as a genetic hub and that Eaf1 is essential for complex integrity.
Molecular and cellular biology
Mitchell L,Lambert JP,Gerdes M,Al-Madhoun AS,Skerjanc IS,Figeys D,Baetz K
A-5800 was used in western blot to study the cellular processes impacted by NuA4
Tue Apr 01 00:00:00 EDT 2008
Functional dissection of the NuA4 histone acetyltransferase reveals its role as a genetic hub and that Eaf1 is essential for complex integrity.
Molecular and cellular biology
Mitchell L,Lambert JP,Gerdes M,Al-Madhoun AS,Skerjanc IS,Figeys D,Baetz K
A-5800 was used in western blot to study the cellular processes impacted by NuA4
Tue Apr 01 00:00:00 EDT 2008
A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis.
Cell
Nachury MV,Loktev AV,Zhang Q,Westlake CJ,Peränen J,Merdes A,Slusarski DC,Scheller RH,Bazan JF,Sheffield VC,Jackson PK
A-5800 was used in Protein-lipid overlay assays to show that the vast majority of evolutionarily conserved Bardet-Biedl syndrome proteins are present in a stable complex that transiently associates with PCM-1.
Fri Jun 15 00:00:00 EDT 2007
MAD-CT-2 identified as a novel melanoma cancer-testis antigen using phage immunoblot analysis.
Journal of immunotherapy (Hagerstown, Md. : 1997)
Dubovsky JA,Albertini MR,McNeel DG
A-5800 was used in western blot to identify MAD-CT-2 as a novel melanoma cancer-testis antigens
Mon Oct 01 00:00:00 EDT 2007
Discovery of highly potent and selective benzyloxybenzyl-based peroxisome proliferator-activator receptor (PPAR) delta agonists.
Bioorganic & medicinal chemistry letters
Bratton LD,Filzen GF,Geyer A,Hoffman JK,Lu G,Pulaski J,Trivedi BK,Unangst PC,Xu X
A-5800 was used to characterize a PPAR modulator
Sun Jul 01 00:00:00 EDT 2007
Synthesis and SAR of selective benzothiophene, benzofuran, and indole-based peroxisome proliferator-activated receptor delta agonists.
Bioorganic & medicinal chemistry letters
Filzen GF,Bratton L,Cheng XM,Erasga N,Geyer A,Lee C,Lu G,Pulaski J,Sorenson RJ,Unangst PC,Trivedi BK,Xu X
A-5800 was used to develop and characterize novel and selective PPARdelta agonists
Sun Jul 01 00:00:00 EDT 2007
TRAPPII subunits are required for the specificity switch of a Ypt-Rab GEF.
Nature cell biology
Morozova N,Liang Y,Tokarev AA,Chen SH,Cox R,Andrejic J,Lipatova Z,Sciorra VA,Emr SD,Segev N
A-5800 was used in western blot to investigate proteins that regulate Golgi entry and exit
Wed Nov 01 00:00:00 EST 2006
Erythrocyte G protein as a novel target for malarial chemotherapy.
PLoS medicine
Murphy SC,Harrison T,Hamm HE,Lomasney JW,Mohandas N,Haldar K
Published figure using GST Tag polyclonal antibody (Product # A-5800) in Western Blot
Fri Dec 01 00:00:00 EST 2006
Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin.
The EMBO journal
Soosairajah J,Maiti S,Wiggan O,Sarmiere P,Moussi N,Sarcevic B,Sampath R,Bamburg JR,Bernard O
A-5800 was used in western blot to identify and characterize an ADF/cofilin phosphoregulatory complex
Wed Feb 09 00:00:00 EST 2005
A combinatorial code for the interaction of alpha-synuclein with membranes.
The Journal of biological chemistry
Kubo S,Nemani VM,Chalkley RJ,Anthony MD,Hattori N,Mizuno Y,Edwards RH,Fortin DL
A-5800 was used in western blot to address how alpha-synuclein interacts with lipid rafts
Fri Sep 09 00:00:00 EDT 2005
Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates.
Science (New York, N.Y.)
Elia AE,Cantley LC,Yaffe MB
A-5800 was used in immunocytochemistry to identify phosphopeptide binding domains that modulate kinase-dependent signaling pathways
Fri Feb 21 00:00:00 EST 2003
Nonradioactive methods for the assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of signaling lipids in cell and tissue extracts.
Analytical biochemistry
Gray A,Olsson H,Batty IH,Priganica L,Peter Downes C
A-5800 was used to develop a technique to quantify phosphoinositides in cell extracts and in vitro enzyme-catalyzed reactions
Sat Feb 15 00:00:00 EST 2003
Elucidation of Smad requirement in transforming growth factor-beta type I receptor-induced responses.
The Journal of biological chemistry
Itoh S,Thorikay M,Kowanetz M,Moustakas A,Itoh F,Heldin CH,ten Dijke P
A-5800 was used in western blot to study the requirement in transforming growth factor-beta type 1 receptor-induced responses by Smad
Fri Feb 07 00:00:00 EST 2003
Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export.
The Journal of cell biology
Frosst P,Guan T,Subauste C,Hahn K,Gerace L
A-5800 was used in immunocytochemistry to analyze the role in nuclear protein export by Trp that is localized within the nuclear basket of the pore complex
Mon Feb 18 00:00:00 EST 2002
Physical properties determining self-organization of motors and microtubules.
Science (New York, N.Y.)
Surrey T,Nedelec F,Leibler S,Karsenti E
A-5800 was used to assess how the concentrations and kinetic parameters of motor proteins contribute to their collective behavior
Fri May 11 00:00:00 EDT 2001
Global analysis of protein activities using proteome chips.
Science (New York, N.Y.)
Zhu H,Bilgin M,Bangham R,Hall D,Casamayor A,Bertone P,Lan N,Jansen R,Bidlingmaier S,Houfek T,Mitchell T,Miller P,Dean RA,Gerstein M,Snyder M
A-5800 was used to utilize proteome chips for global analysis of protein activities
Fri Sep 14 00:00:00 EDT 2001
An effector region in Eps8 is responsible for the activation of the Rac-specific GEF activity of Sos-1 and for the proper localization of the Rac-based actin-polymerizing machine.
The Journal of cell biology
Scita G,Tenca P,Areces LB,Tocchetti A,Frittoli E,Giardina G,Ponzanelli I,Sini P,Innocenti M,Di Fiore PP
A-5800 was used in western blot to learn the responsibility for the activation of the Rac-specific GEF activity of Sos-1 and for the proper localization of the Rac-based actin-polymerizing machine by an effector region in Eps8
Mon Sep 03 00:00:00 EDT 2001
Vps34p differentially regulates endocytosis from the apical and basolateral domains in polarized hepatic cells.
The Journal of cell biology
Tuma PL,Nyasae LK,Backer JM,Hubbard AL
A-5800 was used in immunocytochemistry to elucidate the differential regulation of endocytosis from the apical and basolateral domains in polarized hepatic cells by Vps34p
Mon Sep 17 00:00:00 EDT 2001
Modulation of oncogenic DBL activity by phosphoinositol phosphate binding to pleckstrin homology domain.
The Journal of biological chemistry
Russo C,Gao Y,Mancini P,Vanni C,Porotto M,Falasca M,Torrisi MR,Zheng Y,Eva A
A-5800 was used in immunocytochemistry to elucidate how phosphoinositide modifications influence the function of the Dbl family guanine nucleotide exchange factors
Fri Jun 01 00:00:00 EDT 2001
Multiple levels of control regulate the yeast cAMP-response element-binding protein repressor Sko1p in response to stress.
The Journal of biological chemistry
Pascual-Ahuir A,Posas F,Serrano R,Proft M
A-5800 was used in immunocytochemistry to study regulation of the yeast cAMP-response element-binding protein repressor Sko1p in response to stress by multiple levels of control
Fri Oct 05 00:00:00 EDT 2001
Prenylated Rab acceptor protein is a receptor for prenylated small GTPases.
The Journal of biological chemistry
Figueroa C,Taylor J,Vojtek AB
A-5800 was used in immunocytochemistry and western blot to study the cellular effects of prenylated Rab acceptor protein interactions with Ha-Ras, RhoA, TC21, and Rap1a
Fri Jul 27 00:00:00 EDT 2001
Rim1 and rabphilin-3 bind Rab3-GTP by composite determinants partially related through N-terminal alpha -helix motifs.
The Journal of biological chemistry
Wang X,Hu B,Zimmermann B,Kilimann MW
A-5800 was used to identify amino acids and motifs in Rim1 that are responsible for Rab3 binding
Fri Aug 31 00:00:00 EDT 2001
Identification of another actin-related protein (Arp) 2/3 complex binding site in neural Wiskott-Aldrich syndrome protein (N-WASP) that complements actin polymerization induced by the Arp2/3 complex activating (VCA) domain of N-WASP.
The Journal of biological chemistry
Suetsugu S,Miki H,Takenawa T
A-5800 was used to study an additional actin-related protein (Arp) 2/3 complex binding site in neural Wiskott-Aldrich syndrome protein (N-WASP) that helps with actin polymerization induced by the Arp2/3 complex activating (VCA) domain of N-WASP
Fri Aug 31 00:00:00 EDT 2001
Syndapin isoforms participate in receptor-mediated endocytosis and actin organization.
The Journal of cell biology
Qualmann B,Kelly RB
A-5800 was used in western blot to identify and characterize Syndapin II
Mon Mar 06 00:00:00 EST 2000
Paxillin binding is not the sole determinant of focal adhesion localization or dominant-negative activity of focal adhesion kinase/focal adhesion kinase-related nonkinase.
Molecular biology of the cell
Cooley MA,Broome JM,Ohngemach C,Romer LH,Schaller MD
A-5800 was used in western blot to determine the focal adhesion-targeting sequence of focal adhesion kinase
Fri Sep 01 00:00:00 EDT 2000
Head and/or CaaX domain deletions of lamin proteins disrupt preformed lamin A and C but not lamin B structure in mammalian cells.
Molecular biology of the cell
Izumi M,Vaughan OA,Hutchison CJ,Gilbert DM
A-5800 was used in western blot to examine the effect of lamin A and B1 loss on the lamina structure
Fri Dec 01 00:00:00 EST 2000
Specificity of the binding of synapsin I to Src homology 3 domains.
The Journal of biological chemistry
Onofri F,Giovedi S,Kao HT,Valtorta F,Bongiorno Borbone L,De Camilli P,Greengard P,Benfenati F
A-5800 was used in western blot to investigate the interactions between synapsins and an array of SH3 domains
Fri Sep 22 00:00:00 EDT 2000
Development of a ubiquitin transfer assay for high throughput screening by fluorescence resonance energy transfer.
Journal of biomolecular screening
Boisclair MD,McClure C,Josiah S,Glass S,Bottomley S,Kamerkar S,Hemmilä I
A-5800 was used to screen for ubiquitination inhibitors using fluorescence resonance energy transfer
Sun Oct 01 00:00:00 EDT 2000
The polo-box-dependent induction of ectopic septal structures by a mammalian polo kinase, plk, in Saccharomyces cerevisiae.
Proceedings of the National Academy of Sciences of the United States of America
Lee KS,Song S,Erikson RL
A-5800 was used in western blot to investigate the contribution of Plk to cytokinetic events
Tue Dec 07 00:00:00 EST 1999
Pig-n, a mammalian homologue of yeast Mcd4p, is involved in transferring phosphoethanolamine to the first mannose of the glycosylphosphatidylinositol.
The Journal of biological chemistry
Hong Y,Maeda Y,Watanabe R,Ohishi K,Mishkind M,Riezman H,Kinoshita T
A-5800 was used in western blot to study the phosphatidylinositol glycan-class N gene
Fri Dec 03 00:00:00 EST 1999
Effect of potent and selective inhibitors of the Grb2 SH2 domain on cell motility.
The Journal of biological chemistry
Gay B,Suarez S,Weber C,Rahuel J,Fabbro D,Furet P,Caravatti G,Schoepfer J
A-5800 was used in immunocytochemistry to demonstrate a direct role for Grb2 in cell motility
Fri Aug 13 00:00:00 EDT 1999
Determination of the functional domain organization of the importin alpha nuclear import factor.
The Journal of cell biology
Herold A,Truant R,Wiegand H,Cullen BR
A-5800 was used in immunocytochemistry to characterize the functional domains present in Imp alpha
Mon Oct 19 00:00:00 EDT 1998
Syndecan-4 proteoglycan regulates the distribution and activity of protein kinase C.
The Journal of biological chemistry
Oh ES,Woods A,Couchman JR
A-5800 was used in western blot to report that syndecan-4 is a focal adhesion component
Fri Mar 28 00:00:00 EST 1997
Xklp2, a novel Xenopus centrosomal kinesin-like protein required for centrosome separation during mitosis.
Cell
Boleti H,Karsenti E,Vernos I
A-5800 was used in immunocytochemistry to examine the localization of Xklp2 during the cell cycle
Fri Jan 12 00:00:00 EST 1996
Two independent domains of hDlg are sufficient for subcellular targeting: the PDZ1-2 conformational unit and an alternatively spliced domain.
The Journal of cell biology
Lue RA,Brandin E,Chan EP,Branton D
A-5800 was used in immunocytochemistry to determine the functional role of both PSD-95/Dlg/ZO-1 4.1 binding sites
Fri Nov 01 00:00:00 EST 1996
Rho-stimulated contractility drives the formation of stress fibers and focal adhesions.
The Journal of cell biology
Chrzanowska-Wodnicka M,Burridge K
A-5800 was used in immunocytochemistry to test if rho's induction of stress fibers, focal adhesions, and tyrosine phosphorylation is the result of its stimulation of contractility
Sat Jun 01 00:00:00 EDT 1996
Inhibition of focal adhesion kinase (FAK) signaling in focal adhesions decreases cell motility and proliferation.
Molecular biology of the cell
Gilmore AP,Romer LH
A-5800 was used in immunocytochemistry to determine the role of focal adhesion kinase in focal adhesion structure and function
Thu Aug 01 00:00:00 EDT 1996
Equilibrium and kinetic measurements reveal rapidly reversible binding of Ras to Raf.
The Journal of biological chemistry
Gorman C,Skinner RH,Skelly JV,Neidle S,Lowe PN
A-5800 was used in ELISA and western blot to describe the interaction of the Ras-binding domain of Raf with Ras
Fri Mar 22 00:00:00 EST 1996
Cell-cycle control linked to extracellular environment by MAP kinase pathway in fission yeast.
Nature
Shiozaki K,Russell P
A-5800 was used in western blot to identify a fission yeast MAP kinase pathway links the cell-cycle G2/M control with changes in the extracellular environment that affect cell physiology
Thu Dec 14 00:00:00 EST 1995
