Invitrogen

GST Tag Polyclonal Antibody

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GST Tag Antibody (CAB4169) in WB

Western blot analysis was performed on recombinant proteins (1 µg) of Neurophilin 2 (Lane 1), DOT1 (Lane 2) and GST Tag (Lane 3). The blots were probed with Anti-GST Tag Rabbit Polyclonal Antibody (Product # CAB4169, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 26 kDa band corresponding to GST Tag was observed while different GST tagged proteins showed their corresponding molecular weights. Known quantity of protein samples were ele... View More

GST Tag Antibody in Immunoprecipitation (IP)

GST Tag Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

CAB4169

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 9 publications 9 publications

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Peptide Array (Array)

View 1 publication 1 publication

In vitro Assay (IV)

View 1 publication 1 publication

GST Pull Down (GPD)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified recombinant GST.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

BBS, pH 8.4

Contains

no preservative

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10709998

Product Specific Information

CAB4169 detects GST in samples.

CAB4169 has been successfully used in Western Blot applications.

The CAB4169 immunogen is purified recombinant GST.

Target Information

GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A conformational switch in the SCF-D3/MAX2 ubiquitin ligase facilitates strigolactone signalling.

Nature plants

Tal L,Palayam M,Ron M,Young A,Britt A,Shabek N

CAB4169 was used in Western Blotting to elucidate the functional dynamics of ASK1-D3/MAX2 in SL signalling by leveraging conformational switch mutants in vitro and in plants.

Sun May 01 00:00:00 EDT 2022

Antibodies against human endogenous retrovirus K102 envelope activate neutrophils in systemic lupus erythematosus.

The Journal of experimental medicine

Tokuyama M,Gunn BM,Venkataraman A,Kong Y,Kang I,Rakib T,Townsend MJ,Costenbader KH,Alter G,Iwasaki A

Published figure using GST Tag polyclonal antibody (Product # CAB4169) in Western Blot

Mon Jul 05 00:00:00 EDT 2021

Antibodies against human endogenous retrovirus K102 envelope activate neutrophils in systemic lupus erythematosus.

The Journal of experimental medicine

Tokuyama M,Gunn BM,Venkataraman A,Kong Y,Kang I,Rakib T,Townsend MJ,Costenbader KH,Alter G,Iwasaki A

Published figure using GST Tag polyclonal antibody (Product # CAB4169) in Western Blot

Mon Jul 05 00:00:00 EDT 2021

Discovery of a Novel CIP2A Variant (NOCIVA) with Clinical Relevance in Predicting TKI Resistance in Myeloid Leukemias.

Clinical cancer research : an official journal of the American Association for Cancer Research

Mäkelä E,Pavic K,Varila T,Salmenniemi U,Löyttyniemi E,Nagelli SG,Ammunét T,Kähäri VM,Clark RE,Elo LL,Bachanaboyina VK,Lucas CM,Itälä-Remes M,Westermarck J

CAB4169 was used in GST Pull Down to discover a novel variant of the oncoprotein CIP2A and its clinical relevance in predicting tyrosine kinase inhibitor therapy resistance in myeloid leukemias.

Sat May 15 00:00:00 EDT 2021

Discovery of a Novel CIP2A Variant (NOCIVA) with Clinical Relevance in Predicting TKI Resistance in Myeloid Leukemias.

Clinical cancer research : an official journal of the American Association for Cancer Research

Mäkelä E,Pavic K,Varila T,Salmenniemi U,Löyttyniemi E,Nagelli SG,Ammunét T,Kähäri VM,Clark RE,Elo LL,Bachanaboyina VK,Lucas CM,Itälä-Remes M,Westermarck J

CAB4169 was used in GST Pull Down to discover a novel variant of the oncoprotein CIP2A and its clinical relevance in predicting tyrosine kinase inhibitor therapy resistance in myeloid leukemias.

Sat May 15 00:00:00 EDT 2021

EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis.

Nature genetics

Yang Z,Qian S,Scheid RN,Lu L,Chen X,Liu R,Du X,Lv X,Boersma MD,Scalf M,Smith LM,Denu JM,Du J,Zhong X

CAB4169 was used in in vitro experiments to identify a bivalent chromatin reader capable of recognizing two antagonistic histone marks.

Sat Sep 01 00:00:00 EDT 2018

EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis.

Nature genetics

Yang Z,Qian S,Scheid RN,Lu L,Chen X,Liu R,Du X,Lv X,Boersma MD,Scalf M,Smith LM,Denu JM,Du J,Zhong X

CAB4169 was used in in vitro experiments to identify a bivalent chromatin reader capable of recognizing two antagonistic histone marks.

Sat Sep 01 00:00:00 EDT 2018

EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis.

Nature genetics

Yang Z,Qian S,Scheid RN,Lu L,Chen X,Liu R,Du X,Lv X,Boersma MD,Scalf M,Smith LM,Denu JM,Du J,Zhong X

CAB4169 was used in in vitro experiments to identify a bivalent chromatin reader capable of recognizing two antagonistic histone marks.

Sat Sep 01 00:00:00 EDT 2018

Dual recognition of H3K4me3 and H3K27me3 by a plant histone reader SHL.

Nature communications

Qian S,Lv X,Scheid RN,Lu L,Yang Z,Chen W,Liu R,Boersma MD,Denu JM,Zhong X,Du J

CAB4169 was used in a Peptide array to establish bromo-adjacent homology-plant homeodomain cassette as a dual histone methyl-lysine binding module that recognizes both active and repressive histone marks.

Thu Jun 21 00:00:00 EDT 2018

Long disordered regions of the C-terminal domain of Abelson tyrosine kinase have specific and additive functions in regulation and axon localization.

PloS one

Cheong HSJ,VanBerkum MFA

CAB4169 was used in Western Blotting to examine the functional role of the long c-terminal domain of Abl.

Thu Jan 04 00:00:00 EST 2018

Repo-Man/PP1 regulates heterochromatin formation in interphase.

Nature communications

de Castro IJ,Budzak J,Di Giacinto ML,Ligammari L,Gokhan E,Spanos C,Moralli D,Richardson C,de Las Heras JI,Salatino S,Schirmer EC,Ullman KS,Bickmore WA,Green C,Rappsilber J,Lamble S,Goldberg MW,Vinciotti V,Vagnarelli P

CAB4169 was used in western blot to study the role of Repo-Man in interphase

Mon Jan 16 00:00:00 EST 2017

Breast cancer metastasis suppressor 1 modulates SIRT1-dependent p53 deacetylation through interacting with DBC1.

American journal of cancer research

Liu X,Ehmed E,Li B,Dou J,Qiao X,Jiang W,Yang X,Qiao S,Wu Y

Published figure using GST Tag polyclonal antibody (Product # CAB4169) in Western Blot

Tue Jul 19 00:00:00 EDT 2016

The yeast Rab GTPase Ypt1 modulates unfolded protein response dynamics by regulating the stability of HAC1 RNA.

PLoS genetics

Tsvetanova NG,Riordan DP,Brown PO

Published figure using GST Tag polyclonal antibody (Product # CAB4169) in Western Blot

Fri Jan 04 00:00:00 EST 2013

Cool-1/βPIX functions as a guanine nucleotide exchange factor in the cycling of Cdc42 to regulate insulin secretion.

American journal of physiology. Endocrinology and metabolism

Kepner EM,Yoder SM,Oh E,Kalwat MA,Wang Z,Quilliam LA,Thurmond DC

CAB4169 was used in immunoprecipitation to identify the interacting partners of Cdc42 and their combined effects on insulin release

Thu Dec 01 00:00:00 EST 2011

Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis.

The Journal of biological chemistry

Wiseman DA,Kalwat MA,Thurmond DC

CAB4169 was used in western blot to investigate the effect of syntaxin 4 S-nitrosylation on insulin granule exocytosis

Fri May 06 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GST Tag Polyclonal Antibody

GST Tag Antibody (CAB4169) in WB

Product Details

CAB4169

Western Blot (WB)

Immunoprecipitation (IP)

Peptide Array (Array)

In vitro Assay (IV)

GST Pull Down (GPD)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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