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Invitrogen
GST Tag Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
GST Tag Antibody (PA1-982A) in WB
Western blot analysis of GST was performed by loading 25 µg of 293t cells transfected with GST-MART1 (lane 1), HepG2 cells (lane 2) and 293t cells transfected with 12Tag (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a GST Epitope Tag polyclonal antibody (Product # PA1-982A) at a dilution of 1:5000 (GST and 12Tag) and 1:1000 (HepG2) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperat... View More
Product Details
PA1-982A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
GST-tagged fusion proteins.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1074693
Product Specific Information
PA1-982A detects glutathione-S-transferase (GST).
PA1-982A has been successfully used in Western blot procedures to detect the presence of GST and GST-tagged proteins.
PA1-982A antigen is GST-tagged fusion protein.
Target Information
GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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