Proteintech

GST Tag Monoclonal Antibody (3G12B10), HRP

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GST Tag Antibody (HRP-66001) in WB

Recombinant protein were subjected to SDS PAGE followed by western blot with GST Tag antibody Product # HRP-66001) at dilution of 1:20,000 incubated at room temperature for 1.5 hours.

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Product Details

HRP-66001

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 17 publications 17 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3G12B10

Immunogen

Recombinant protein

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

0.67 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.5% BSA

Contains

0.02% thimerosal, 0.05% ProClin 300

Storage conditions

-20° C, store in dark

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add azide (Sodium Azide or Smite) to the dilution buffer. azide is the HRP inhibitor which decreases the enzymatic activity of HRP.

Target Information

GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GST Tag Monoclonal Antibody (3G12B10), HRP

GST Tag Antibody (HRP-66001) in WB

Product Details

HRP-66001

Western Blot (WB)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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