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Invitrogen
GST Tag Monoclonal Antibody (8-326), Alexa Fluor™ 488
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
GST Tag Antibody (MA4-004-A488) in ICC/IF
Immunofluorescent analysis of HeLa cells transfected with a construct containing a GST Epitope Tag. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with AlexaFluor 488 conjugated GST-tag monoclonal antibody (Product # MA4-004-A488) at a dilution of 1:25 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were... View More
Product Details
MA4-004-A488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8-326
Immunogen
Recombinant purified full-length GST
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610641
Target Information
GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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